Polymicrobial Interactions in the Urinary Tract: Is the Enemy of My Enemy My Friend?

2021 ◽  
Vol 89 (4) ◽  
Author(s):  
Jordan R. Gaston ◽  
Alexandra O. Johnson ◽  
Kirsten L. Bair ◽  
Ashley N. White ◽  
Chelsie E. Armbruster
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Catheters ◽  
Content Type

The vast majority of research pertaining to urinary tract infection has focused on a single pathogen in isolation and predominantly Escherichia coli . However, polymicrobial urine colonization and infections are prevalent in several patient populations, including individuals with urinary catheters.

scholarly journals Complete Genome Sequences of Seven Uropathogenic Escherichia coli Strains Isolated from Postmenopausal Women with Recurrent Urinary Tract Infection

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Belle M. Sharon ◽  
Amber Nguyen ◽  
Amanda P. Arute ◽  
Neha V. Hulyalkar ◽  
Vivian H. Nguyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Postmenopausal Women ◽  
Complete Genome ◽  
Recurrent Urinary Tract Infection ◽  
Uropathogenic Escherichia Coli ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infection (UTI). This disease disproportionately affects women and frequently develops into recurrent UTI (rUTI) in postmenopausal women. Here, we report the complete genome sequences of seven UPEC isolates obtained from the urine of postmenopausal women with rUTI.

scholarly journals Draft Genome Sequence of an Escherichia coli Sequence Type 420 Isolate from a Patient with Urinary Tract Infection in Northern California

2020 ◽  
Vol 9 (23) ◽  
Author(s):  
Yusuke Matsui ◽  
Yuan Hu ◽  
Nicole J. Tarlton ◽  
Lee W. Riley
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Genome Sequence ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Northern California ◽  
Content Type

ABSTRACT The genome sequence of a uropathogenic Escherichia coli sequence type 420 strain isolated from a patient with urinary tract infection in northern California is described here. The draft genome sequence includes a 4.8-Mb chromosome, accompanied by a 114-kb plasmid containing IncFIB/IncFII/Col156 and a 35-kb plasmid containing IncN3.

scholarly journals Escherichia coli Isolates That Carryvat,fyuA,chuA, andyfcVEfficiently Colonize the Urinary Tract

2012 ◽  
Vol 80 (12) ◽  
pp. 4115-4122 ◽  
Author(s):  
Rachel R. Spurbeck ◽  
Paul C. Dinh ◽  
Seth T. Walk ◽  
Ann E. Stapleton ◽  
Thomas M. Hooton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Neonatal Meningitis ◽  
Content Type ◽  
E Coli ◽  
Core Set ◽  
Encoding Genes

ABSTRACTExtraintestinalEscherichia coli(ExPEC), a heterogeneous group of pathogens, encompasses avian, neonatal meningitis, and uropathogenicE. colistrains. While several virulence factors are associated with ExPEC, there is no core set of virulence factors that can be used to definitively differentiate these pathotypes. Here we describe a multiplex of four virulence factor-encoding genes,yfcV,vat,fyuA, andchuA, highly associated with uropathogenicE. colistrains that can distinguish three groups ofE. coli: diarrheagenic and animal-associatedE. colistrains, human commensal and avian pathogenicE. colistrains, and uropathogenic and neonatal meningitisE. colistrains. Furthermore, human intestinal isolates that encode all four predictor genes express them during exponential growth in human urine and colonize the bladder in the mouse model of ascending urinary tract infection in higher numbers than human commensal strains that do not encode the four predictor genes (P= 0.02), suggesting that the presence of the predictors correlates with uropathogenic potential.

scholarly journals Immune Modulation by Group B Streptococcus Influences Host Susceptibility to Urinary Tract Infection by Uropathogenic Escherichia coli

2012 ◽  
Vol 80 (12) ◽  
pp. 4186-4194 ◽  
Author(s):  
Kimberly A. Kline ◽  
Drew J. Schwartz ◽  
Nicole M. Gilbert ◽  
Scott J. Hultgren ◽  
Amanda L. Lewis
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Immune Modulation ◽  
Inflammatory Responses ◽  
Acute Infection ◽  
Host Susceptibility ◽  
Content Type ◽  
Group B

ABSTRACTUrinary tract infection (UTI) is most often caused by uropathogenicEscherichia coli(UPEC). UPEC inoculation into the female urinary tract (UT) can occur through physical activities that expose the UT to an inherently polymicrobial periurethral, vaginal, or gastrointestinal flora. We report that a common urogenital inhabitant and opportunistic pathogen, group BStreptococcus(GBS), when present at the time of UPEC exposure, undergoes rapid UPEC-dependent exclusion from the murine urinary tract, yet it influences acute UPEC-host interactions and alters host susceptibility to persistent outcomes of bladder and kidney infection. GBS presence results in increased UPEC titers in the bladder lumen during acute infection and reduced inflammatory responses of murine macrophages to live UPEC or purified lipopolysaccharide (LPS), phenotypes that require GBS mimicry of host sialic acid residues. Taken together, these studies suggest that despite low titers, the presence of GBS at the time of polymicrobial UT exposure may be an overlooked risk factor for chronic pyelonephritis and recurrent UTI in susceptible groups, even if it is outcompeted and thus absent by the time of diagnosis.

scholarly journals Ciprofloxacin Pharmacokinetics/Pharmacodynamics against Susceptible and Low-Level Resistant Escherichia coli Isolates in an Experimental Ascending Urinary Tract Infection Model in Mice

2020 ◽  
Vol 65 (1) ◽  
pp. e01804-20
Author(s):  
Lotte Jakobsen ◽  
Carina Vingsbro Lundberg ◽  
Niels Frimodt-Møller
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Infection Model ◽  
Bladder Tissue ◽  
Content Type ◽  
E Coli ◽  
Low Level ◽  
Level Resistance

ABSTRACTThe mouse ascending urinary tract infection model was used to study the pharmacokinetic/pharmacodynamic (PKPD) relationships of the effect of ciprofloxacin in subcutaneous treatment for 3 days with varying doses and dosing intervals against a susceptible Escherichia coli strain (MIC, 0.032 mg/liter). Further, a humanized dose of ciprofloxacin was administered for 3 days against three E. coli strains with low-level resistance, i.e., MICs of 0.06, 0.25, and 1 mg/liter, respectively. Against the susceptible isolate, ciprofloxacin was highly effective in clearing the urine with daily doses from 10 mg/kg, but the dosing regimen had to be divided into at least two doses for optimal effect. Ciprofloxacin could not clear the urine or kidneys for the low-level-resistant strains. PKPD correlations with all strains combined showed that for the AUC24/MIC there was a slightly higher correlation with effect in urine and kidneys (R2, 0.71 and 0.69, respectively) than the %T>MIC (R2, 0.41 and 0.61, respectively). Equal correlations for the two PKPD indices were found for reduction of colony counts (CFU) in the bladder tissue, but not even the highest dose of 28 mg/kg × 6 could clear the bladder tissue. In conclusion, ciprofloxacin is highly effective in clearing the urine and kidney tissue for fully susceptible E. coli, while even low-level resistance in E. coli obscures this effect. While the effect of ciprofloxacin is mostly AUC/MIC driven against E. coli infection in the urinary tract, the effect in urine depends on the presence of ciprofloxacin in the urine during most of a 24-h period.

scholarly journals Antimicrobial Activity Evaluation of Tebipenem (SPR859), an Orally Available Carbapenem, against a Global Set of Enterobacteriaceae Isolates, Including a Challenge Set of Organisms

2019 ◽  
Vol 63 (6) ◽  
Author(s):  
S. J. Ryan Arends ◽  
Paul R. Rhomberg ◽  
Nicole Cotroneo ◽  
Aileen Rubio ◽  
Robert K. Flamm ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Broth Microdilution ◽  
Wild Type ◽  
Content Type ◽  
E Coli

ABSTRACT The antimicrobial activity of tebipenem and other carbapenem agents were tested in vitro against a set of recent clinical isolates responsible for urinary tract infection (UTI), as well as against a challenge set. Isolates were tested by reference broth microdilution and included Escherichia coli (101 isolates), Klebsiella pneumoniae (208 isolates), and Proteus mirabilis (103 isolates) species. Within each species tested, tebipenem showed equivalent MIC50/90 values to those of meropenem (E. coli MIC50/90, ≤0.015/0.03 mg/liter; K. pneumoniae MIC50/90, 0.03/0.06 mg/liter; and P. mirabilis MIC50/90, 0.06/0.12 mg/liter) and consistently displayed MIC90 values 8-fold lower than imipenem. Tebipenem and meropenem (MIC50, 0.03 mg/liter) showed equivalent MIC50 results against wild-type, AmpC-, and/or extended-spectrum β-lactamase (ESBL)-producing isolates. Tebipenem also displayed MIC50/90 values 4- to 8-fold lower than imipenem against the challenge set. All carbapenem agents were less active (MIC50, ≥8 mg/liter) against isolates carrying carbapenemase genes. These data confirm the in vitro activity of the orally available agent tebipenem against prevalent UTI Enterobacteriaceae species, including those producing ESBLs and/or plasmid AmpC enzymes.

scholarly journals Characteristics of NDM-1-Producing Escherichia coli Isolates That Belong to the Successful and Virulent Clone ST131

2011 ◽  
Vol 55 (6) ◽  
pp. 2986-2988 ◽  
Author(s):  
Gisele Peirano ◽  
Paul C. Schreckenberger ◽  
Johann D. D. Pitout
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
The United States ◽  
Phylogenetic Group ◽  
Sequence Type ◽  
Content Type ◽  
E Coli

ABSTRACTAn NDM-1 carbapenemase-producingEscherichia coliisolate of sequence type 131 (ST131) that belonged to phylogenetic group B2 was obtained from a patient with a urinary tract infection who returned to the United States after a recent hospitalization while visiting India. NDM-1-producingE. coliST131 had significantly more virulence factors than NDM-1-producingE. coliST101, previously isolated from a patient in Canada. The presence of NDM β-lactamases in a very successful and virulentE. colisequence type is of concern.

scholarly journals D-Serine Degradation byProteus mirabilisContributes to Fitness during Single-Species and Polymicrobial Catheter-Associated Urinary Tract Infection

mSphere ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Aimee L. Brauer ◽  
Ashley N. White ◽  
Brian S. Learman ◽  
Alexandra O. Johnson ◽  
Chelsie E. Armbruster
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Proteus Mirabilis ◽  
Time Course ◽  
Single Species ◽  
Morganella Morganii ◽  
Content Type ◽  
Providencia Stuartii

ABSTRACTProteus mirabilisis a common cause of catheter-associated urinary tract infection (CAUTI) and secondary bacteremia, which are frequently polymicrobial. We previously utilized transposon insertion-site sequencing (Tn-Seq) to identify novel fitness factors for colonization of the catheterized urinary tract during single-species and polymicrobial infection, revealing numerous metabolic pathways that may contribute toP. mirabilisfitness regardless of the presence of other cocolonizing organisms. One such “core” fitness factor wasd-serine utilization. In this study, we generated isogenic mutants ind-serine dehydratase (dsdA),d-serine permease (dsdX), and the divergently transcribed activator of the operon (dsdC) to characterized-serine utilization inP. mirabilisand explore the contribution of this pathway to fitness during single-species and polymicrobial infection.P. mirabiliswas capable of utilizing eitherd- orl-serine as a sole carbon or nitrogen source, anddsdA,dsdX, anddsdCwere each specifically required ford-serine degradation. This capability was highly conserved amongP. mirabilisisolates, although not universal among uropathogens:Escherichia coliandMorganella morganiiutilizedd-serine, whileProvidencia stuartiiandEnterococcus faecalisdid not.d-Serine utilization did not contribute toP. mirabilisgrowth in urineex vivoduring a 6-h time course but significantly contributed to fitness during single-species and polymicrobial CAUTI during a 96-h time course, regardless ofd-serine utilization by the coinfecting isolate.d-Serine utilization also contributed to secondary bacteremia during CAUTI as well as survival in a direct bacteremia model. Thus, we proposed-serine utilization as a core fitness factor inP. mirabilisand a possible target for disruption of infection.IMPORTANCEUrinary tract infections are among the most common health care-associated infections worldwide, the majority of which involve a urinary catheter (CAUTI). Our recent investigation of CAUTIs in nursing home residents identifiedProteus mirabilis,Enterococcusspecies, andEscherichia colias the three most common organisms. These infections are also often polymicrobial, and we identifiedMorganella morganii,Enterococcusspecies, andProvidencia stuartiias being more prevalent during polymicrobial CAUTI than single-species infection. Our research therefore focuses on identifying “core” fitness factors that are highly conserved inP. mirabilisand that contribute to infection regardless of the presence of these other organisms. In this study, we determined that the ability to degraded-serine, the most abundantd-amino acid in urine and serum, strongly contributes toP. mirabilisfitness within the urinary tract, even when competing for nutrients with another organism.d-Serine uptake and degradation therefore represent potential targets for disruption ofP. mirabilisinfections.

scholarly journals Genome Sequence of Escherichia coli Clone O25:H4 Sequence Type 131, Isolated from a Sudanese Patient with Urinary Tract Infection

2020 ◽  
Vol 9 (11) ◽  
Author(s):  
Sofia B. Mohamed ◽  
Mohamed M. Hassan ◽  
Sumaya Kambal ◽  
Abdalla Munir ◽  
Nusiba I. Abdalla ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Genome Sequence ◽  
Sequence Type ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
E Coli

We report here the whole-genome sequence of Escherichia coli NUBRI-E, a representative of E. coli clone O25:H4 sequence type 131 with bla CTX-M-15, which was obtained from a Sudanese patient with a urinary tract infection.

scholarly journals Genome Sequence of Escherichia coli KI683, Isolated from a Urosepsis Patient

2020 ◽  
Vol 9 (9) ◽  
Author(s):  
Nathalie Stefani ◽  
Volker Schroeckh ◽  
Ute Neugebauer ◽  
Jürgen Bohnert ◽  
Axel A. Brakhage
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Genome Size ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Content Type

Escherichia coli KI683 was isolated from blood of a patient who developed septicemia as a complication of a urinary tract infection. Genome sequencing resulted in three contigs with a total genome size of 5,243,173 bp encoding 5,143 genes.

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