scholarly journals Carbapenem-Resistant KPC-2-Producing Escherichia coli in a Tel Aviv Medical Center, 2005 to 2008

2010 ◽  
Vol 54 (6) ◽  
pp. 2687-2691 ◽  
Author(s):  
Moran G. Goren ◽  
Shiri Navon-Venezia ◽  
Inna Chmelnitsky ◽  
Yehuda Carmeli
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Medical Center ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Beta Lactamase ◽  
Pulsed Field ◽  
Extended Spectrum Beta Lactamase ◽  
Tel Aviv ◽  
Carbapenem Resistant

ABSTRACT All of the carbapenem-resistant Escherichia coli (CREC) isolates identified in our hospital from 2005 to 2008 (n = 10) were studied. CREC isolates were multidrug resistant, all carried bla KPC-2, and six of them were also extended-spectrum beta-lactamase producers. Pulsed-field gel electrophoresis indicated six genetic clones; within the same clone, similar transferable bla KPC-2-containing plasmids were found whereas plasmids differed between clones. Tn4401 elements were identified in all of these plasmids.

scholarly journals Surveillance of Carbapenem-Resistant Pseudomonas aeruginosa Isolates from Puerto Rican Medical Center Hospitals: Dissemination of KPC and IMP-18 β-Lactamases

2009 ◽  
Vol 53 (4) ◽  
pp. 1660-1664 ◽  
Author(s):  
Daniel J. Wolter ◽  
Noha Khalaf ◽  
Iraida E. Robledo ◽  
Guillermo J. Vázquez ◽  
Maria I. Santé ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Puerto Rican ◽  
Gel Electrophoresis ◽  
Medical Center ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Carbapenem Resistant ◽  
Clonal Spread

ABSTRACT During a 6-month period, 37/513 (7.2%) Pseudomonas aeruginosa isolates belonging to 13 pulsed-field gel electrophoresis (PFGE) groups from Puerto Rican hospitals were carbapenem nonsusceptible. Seven of 37 isolates from four PFGE groups carried bla IMP-18, and 25/37 isolates from seven PFGE groups carried bla KPC. The results indicated the clonal spread of bla KPC-positive P. aeruginosa isolates into several Puerto Rican hospitals and the dissemination of bla IMP-18 and bla KPC into genetically unrelated isolates.

scholarly journals Molecular Epidemiology of Ceftiofur-Resistant Escherichia coli Isolates from Dairy Calves

2006 ◽  
Vol 72 (6) ◽  
pp. 3940-3948 ◽  
Author(s):  
Sarah C. Donaldson ◽  
Beth A. Straley ◽  
Narasimha V. Hegde ◽  
Ashish A. Sawant ◽  
Chitrita DebRoy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Dairy Herd ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Dairy Calves ◽  
Disk Diffusion ◽  
Pulsed Field ◽  
E Coli ◽  
Clonal Type

ABSTRACT Healthy calves (n = 96, 1 to 9 weeks old) from a dairy herd in central Pennsylvania were examined each month over a five-month period for fecal shedding of ceftiofur-resistant gram-negative bacteria. Ceftiofur-resistant Escherichia coli isolates (n = 122) were characterized by antimicrobial resistance (disk diffusion and MIC), serotype, pulsed-field gel electrophoresis subtypes, beta-lactamase genes, and virulence genes. Antibiotic disk diffusion assays showed that the isolates were resistant to ampicillin (100%), ceftiofur (100%), chloramphenicol (94%), florfenicol (93%), gentamicin (89%), spectinomycin (72%), tetracycline (98%), ticarcillin (99%), and ticarcillin-clavulanic acid (99%). All isolates were multidrug resistant and displayed elevated MICs. The E. coli isolates belonged to 42 serotypes, of which O8:H25 was the predominant serotype (49.2%). Pulsed-field gel electrophoresis classified the E. coli isolates into 27 profiles. Cluster analysis showed that 77 isolates (63.1%) belonged to one unique group. The prevalence of pathogenic E. coli was low (8%). A total of 117 ceftiofur-resistant E. coli isolates (96%) possessed the bla CMY2 gene. Based on phenotypic and genotypic characterization, the ceftiofur-resistant E. coli isolates belonged to 59 clonal types. There was no significant relationship between calf age and clonal type. The findings of this study revealed that healthy dairy calves were rapidly colonized by antibiotic-resistant strains of E. coli shortly after birth. The high prevalence of multidrug-resistant nonpathogenic E. coli in calves could be a significant source of resistance genes to other bacteria that share the same environment.

scholarly journals Prevalence and Characterization of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolated in Canadian Hospitals: Results from CANWARD 2007

2009 ◽  
Vol 20 (suppl a) ◽  
pp. 43A-48A ◽  
Author(s):  
Patricia J Baudry ◽  
Melissa McCracken ◽  
Philippe Lagacé-Wiens ◽  
Michael R Mulvey ◽  
George G Zhanel ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Diffusion Method ◽  
Beta Lactamase ◽  
Single Strain ◽  
Pulsed Field ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Esbl Producers

OBJECTIVE: The purpose of the present study was to determine the prevalence and molecular epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae identified from Canadian hospitals in 2007. METHODS: Clinically significant isolates were collected as part of the Canadian Ward Surveillance Study (CANWARD 2007) from January to December 2007, inclusive, from 12 sentinel hospital centres across Canada. Minimum inhibitory concentrations were determined by broth microdilution, and putative ESBL isolates were confirmed by the Clinical and Laboratory Standards Institute disk diffusion method. Polymerase chain reaction and DNA sequencing were used to detectblaSHV,blaTEM,blaCTX-MandblaOXA-likegenes. Strains were typed using pulsed-field gel electrophoresis. RESULTS: A total of 3.4% and 1.6% ofEscherichia coliandKlebsiella pneumoniae, respectively, were identified as ESBL producers. Resistance to fluoroquinolones, doxycycline, trimethoprim/sulfamethoxazole and gentamicin occurred in 92.5% and 71.4%, 75.5% and 71.4%, 67.9% and 57.1%, and 58.5% and 57.1% of ESBL-producingE coliandK pneumoniae, respectively. A total of 90.6% and 71.4% of ESBL-producingE coliandK pneumoniaewere identified as multidrug resistant. The CTX-M type was the predominant ESBL, with CTX-M-15 as the predominant genotype. A total of 81.7% ESBL-producers carried several beta-lactamase genes. Pulsed-field gel electrophoresis revealed that the majority of ESBL producers were not genetically related (less than 80% homology). Similar patient demographics were observed among both ESBL-producingE coliand K pneumoniae. CONCLUSION: CTX-M has become the most common enzyme among both ESBL-producingE coliandK pneumoniae. The spread of ESBLproducing bacteria across Canada is polyclonal and is not due to the clonal spread of a single strain.

scholarly journals Diversity of extended-spectrum beta-lactamase-producing Escherichia coli rods

2017 ◽  
Vol 71 (1) ◽  
pp. 0-0
Author(s):  
Patrycja Zalas-Więcek ◽  
Tomasz Bogiel ◽  
Krzysztof Wiśniewski ◽  
Eugenia Gospodarek-Komkowska
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Susceptibility Testing ◽  
Pulsed Field Gel Electrophoresis ◽  
Beta Lactamase ◽  
Pulsed Field ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Similarity Group

Introduction: The aim of the study was to evaluate genetic relatedness and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing E. coli strains isolated from patients hospitalized in the University Hospital in Bydgoszcz (Poland).Material and methods: The study included 33 extended-spectrum beta-lactamase-producing E. coli strains isolated from 31 patients. The chromosomal DNA was extracted from the strains and separated by pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was performed by the agar dilution method and carried out according to the European Committee on Antimicrobial Susceptibility Testing recommendations.Results: According to the pulsed-field gel electrophoresis results, 32 distinct pulsotypes were revealed. Based on Molecular Analyst Fingerprinting software analysis, the studied isolates were divided into four subgroups: 6 (18.2%) isolates showing similarity greater than 90% (group A); 19 (57.6%) showing 80-90% similarity (group B); 7 (21.2%) showing 70-79% similarity (group C); and one isolate with less than 70% similarity (group D). Among E. coli isolates showing similarity greater than 90%, four antimicrobial patterns were noted. Among the isolates showing 80-90% similarity, 18 antimicrobial patterns were observed. E. coli isolates showing 70-79% similarity presented 6 antimicrobial patterns.Conclusions: Our results show a high degree of genetic diversity of extended-spectrum beta-lactamase-producing E. coli isolates. However, based on a similarity of ≥80%, almost 75% of E. coli isolates were clonally related. Although it is difficult to identify definitive transmission events based on the recovery of indistinguishable pulsed-field gel electrophoresis types alone, we speculate that extended-spectrum beta-lactamase-producing E. coli strains may have disseminated throughout the hospital.

Sign in / Sign up

Export Citation Format

Share Document