Roles of Calcineurin and Crz1 in Antifungal Susceptibility and Virulence of Candida glabrata

ABSTRACT A Candida glabrata calcineurin mutant exhibited increased susceptibility to both azole antifungal and cell wall-damaging agents and was also attenuated in virulence. Although a mutant lacking the downstream transcription factor Crz1 displayed a cell wall-associated phenotype intermediate to that of the calcineurin mutant and was modestly attenuated in virulence, it did not show increased azole susceptibility. These results suggest that calcineurin regulates both Crz1-dependent and -independent pathways depending on the type of stress.

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A Banana PHD-Type Transcription Factor MaPHD1 Represses a Cell Wall-Degradation Gene MaXTH6 during Fruit Ripening

Horticultural Plant Journal ◽

10.1016/j.hpj.2017.08.003 ◽

2017 ◽

Vol 3 (5) ◽

pp. 190-198 ◽

Cited By ~ 2

Author(s):

Wei WEI ◽

Zhongqi FAN ◽

Jianye CHEN ◽

Jianfei KUANG ◽

Wangjin LU ◽

...

Keyword(s):

Cell Wall ◽

Transcription Factor ◽

Fruit Ripening ◽

Cell Wall Degradation ◽

A Cell

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A family of glycosylphosphatidylinositol-linked aspartyl proteases is required for virulence of Candida glabrata

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0611195104 ◽

2007 ◽

Vol 104 (18) ◽

pp. 7628-7633 ◽

Cited By ~ 179

Author(s):

Rupinder Kaur ◽

Biao Ma ◽

Brendan P. Cormack

Keyword(s):

Cell Wall ◽

Mammalian Cells ◽

Candida Glabrata ◽

Global Changes ◽

Cell Wall Proteins ◽

Culture Model ◽

Genes Encoding ◽

Tissue Culture Model ◽

A Cell ◽

Aspartyl Proteases

Candida glabrata is a yeast pathogen of humans. We have established a tissue culture model to analyze the interaction of C. glabrata with macrophages. Transcript profiling of yeast ingested by macrophages reveals global changes in metabolism as well as increased expression of a gene family (YPS genes) encoding extracellular glycosylphosphatidylinositol-linked aspartyl proteases. Eight of these YPS genes are found in a cluster that is unique to C. glabrata. Genetic analysis shows that the C. glabrata YPS genes are required for cell wall integrity, adherence to mammalian cells, survival in macrophages and virulence. By monitoring the processing of a cell wall adhesin, Epa1, we also show that Yps proteases play an important role in cell wall re-modeling by removal and release of glycosylphosphatidylinositol-anchored cell wall proteins.

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TheCandida albicansDse1 Protein Is Essential and Plays a Role in Cell Wall Rigidity, Biofilm Formation, and Virulence

Interdisciplinary Perspectives on Infectious Diseases ◽

10.1155/2011/504280 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Jalil Y. Daher ◽

Joseph Koussa ◽

Samer Younes ◽

Roy A. Khalaf

Keyword(s):

Cell Wall ◽

Biofilm Formation ◽

Essential Gene ◽

Calcofluor White ◽

Causative Agents ◽

A Cell ◽

Cell Wall Rigidity ◽

Inducing Factors ◽

Mouse Model Of Infection ◽

Increased Susceptibility

The fungal pathogenCandida albicansis one of the leading causative agents of death in immunocompromised individuals. It harbors an arsenal of cell wall anchored factors that are implicated in virulence such as filamentation inducing factors, adhesins, lipases, proteases, and superoxide dismutases. Dse1 is a cell wall protein involved in cell wall metabolism. The purpose of this study is to characterize the role Dse1 plays in virulence. Dse1 appears to be an essential gene as no homozygous null mutant was possible. The heterozygote mutant exhibited increased susceptibility to calcofluor white, a cell wall disrupting agent, with a subsequent reduction in cell wall chitin content, decreased oxidative stress tolerance, a 30% reduction in biofilm formation, and a delay in adhesion that was mirrored by a reduction in virulence in a mouse model of infection. Dse1 thus appears to be an important protein involved in cell wall integrity and rigidity.

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Structural study of a cell wall mannan-protein complex of the pathogenic yeast Candida glabrata IFO 0622 strain

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(92)90739-j ◽

1992 ◽

Vol 294 (2) ◽

pp. 662-669 ◽

Cited By ~ 29

Author(s):

Hidemitsu Kobayashi ◽

Hideko Mitobe ◽

Kaori Takahashi ◽

Takayuki Yamamoto ◽

Nobuyuki Shibata ◽

...

Keyword(s):

Cell Wall ◽

Structural Study ◽

Protein Complex ◽

Candida Glabrata ◽

Pathogenic Yeast ◽

A Cell ◽

Cell Wall Mannan

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Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection

BMC Plant Biology ◽

10.1186/s12870-018-1391-5 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 11

Author(s):

Shakuntala E. Pillai ◽

Chandan Kumar ◽

Hitendra K. Patel ◽

Ramesh V. Sonti

Keyword(s):

Cell Wall ◽

Transcription Factor ◽

Salt Stress ◽

Bacterial Infection ◽

Callose Deposition ◽

Cell Wall Damage ◽

A Cell

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Characterization of a cell wall beta-galactosidase of Cicer arietinum epicotyls involved in cell wall autolysis

Physiologia Plantarum ◽

10.1034/j.1399-3054.1990.800421.x ◽

1990 ◽

Vol 80 (4) ◽

pp. 629-635 ◽

Cited By ~ 1

Author(s):

Berta Dopico ◽

Gregorio Nicolas ◽

Emilia Labrador

Keyword(s):

Cell Wall ◽

Cicer Arietinum ◽

A Cell ◽

Beta Galactosidase

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Stimulation of ethylene production by a cell wall component from mature green tomato fruit

Physiologia Plantarum ◽

10.1034/j.1399-3054.1990.800402.x ◽

1990 ◽

Vol 80 (4) ◽

pp. 500-506 ◽

Cited By ~ 1

Author(s):

Cindy B. S. Tong ◽

Kenneth C. Gross

Keyword(s):

Cell Wall ◽

Ethylene Production ◽

Tomato Fruit ◽

Cell Wall Component ◽

A Cell ◽

Stimulation Of

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Faculty Opinions recommendation of A bacterial effector acts as a plant transcription factor and induces a cell size regulator.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1094879.549818 ◽

2007 ◽

Author(s):

Rino Rappuoli

Keyword(s):

Transcription Factor ◽

Cell Size ◽

A Cell

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An R2R3-type myeloblastosis transcription factor MYB103 is involved in phosphorus remobilization

Food Production, Processing and Nutrition ◽

10.1186/s43014-020-00038-6 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Fangwei Yu ◽

Shenyun Wang ◽

Wei Zhang ◽

Hong Wang ◽

Li Yu ◽

...

Keyword(s):

Cell Wall ◽

Transcription Factor ◽

Abiotic Stresses ◽

Myb Transcription Factor ◽

Ethylene Signaling ◽

Biotic And Abiotic Stresses ◽

P Deficiency ◽

P Concentration ◽

Increased Sensitivity

Abstract The members of myeloblastosis transcription factor (MYB TF) family are involved in the regulation of biotic and abiotic stresses in plants. However, the role of MYB TF in phosphorus remobilization remains largely unexplored. In the present study, we show that an R2R3 type MYB transcription factor, MYB103, is involved in phosphorus (P) remobilization. MYB103 was remarkably induced by P deficiency in cabbage (Brassica oleracea var. capitata L.). As cabbage lacks the proper mutant for elucidating the mechanism of MYB103 in P deficiency, another member of the crucifer family, Arabidopsis thaliana was chosen for further study. The transcript of its homologue AtMYB103 was also elevated in response to P deficiency in A. thaliana, while disruption of AtMYB103 (myb103) exhibited increased sensitivity to P deficiency, accompanied with decreased tissue biomass and soluble P concentration. Furthermore, AtMYB103 was involved in the P reutilization from cell wall, as less P was released from the cell wall in myb103 than in wildtype, coinciding with the reduction of ethylene production. Taken together, our results uncover an important role of MYB103 in the P remobilization, presumably through ethylene signaling.

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