scholarly journals Influence of technical factor variations during inoculum preparation on the agar dilution plate-count method for quantitation of Staphylococcus aureus oxacillin persisters.

1986 ◽  
Vol 30 (5) ◽  
pp. 792-793 ◽  
Author(s):  
B F Woolfrey ◽  
R T Lally ◽  
M N Ederer
Keyword(s):  
Staphylococcus Aureus ◽  
Plate Count ◽  
Technical Factor ◽  
Dilution Plate ◽  
Count Method ◽  
Agar Dilution ◽  
Plate Count Method ◽  
Inoculum Preparation

scholarly journals Comparison of an Automated Technique TEMPO with Direct Plate Count Method for the Enumeration of Staphylococcus aureus

2013 ◽  
Vol 28 (3) ◽  
pp. 252-257
Author(s):  
Yong Sun Cho ◽  
Da-Yeon Lee ◽  
Joo-Young Lee ◽  
Hae-Jin Wang ◽  
Dong-Bin Shin
Keyword(s):  
Staphylococcus Aureus ◽  
Plate Count ◽  
Count Method ◽  
Plate Count Method ◽  
Automated Technique

scholarly journals Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli

2008 ◽  
Vol 74 (7) ◽  
pp. 2171-2178 ◽  
Author(s):  
Woo Kyung Jung ◽  
Hye Cheong Koo ◽  
Ki Woo Kim ◽  
Sook Shin ◽  
So Hyun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mechanism Of Action ◽  
Antibacterial Effect ◽  
Silver Ion ◽  
Plate Count ◽  
E Coli ◽  
Count Method ◽  
Plate Count Method ◽  
Conventional Plate

ABSTRACT The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.

Comparison between the EC plate count test at 21 °C and an accelerated plate count method for assessing the keeping quality of pasteurized milk

1992 ◽  
Vol 59 (3) ◽  
pp. 431-436 ◽  
Author(s):  
Sarah A. Langford ◽  
Rohan G. Kroll
Keyword(s):  
Plate Count ◽  
Gram Negative ◽  
Rapid Methods ◽  
Pasteurized Milk ◽  
Psychrotrophic Bacteria ◽  
Commercial Interest ◽  
Count Method ◽  
Keeping Quality ◽  
Plate Count Method

The keeping quality of properly refrigerated pasteurized milk and cream is primarily determined by post-pasteurization contamination by Gram-negative psychrotrophic bacteria (Phillips et al. 1981; Schröder et al. 1982). Reliable and rapid methods of assessing the levels of contamination by these organisms are therefore of commercial interest.

scholarly journals Comparison of Petrifilm Staph Express Count System with the Bacteriological Analytical Manual Direct-Plating Method for Enumeration of Staphylococcus aureus in Artificially Contaminated Hard Cheese

2008 ◽  
Vol 91 (5) ◽  
pp. 1138-1141 ◽  
Author(s):  
Willis M Fedio ◽  
Chitra N Wendakoon ◽  
Ruben Zapata ◽  
Christina Carrillo ◽  
Paul Browning
Keyword(s):  
Staphylococcus Aureus ◽  
Multivariate Analysis ◽  
Rapid Method ◽  
Analysis Of Variance ◽  
Plate Count ◽  
Direct Plating ◽  
Plating Method ◽  
Plate Count Method ◽  
The U.S ◽  
Hard Cheese

Abstract The 3M Petrifilm Staph Express Count System was compared with the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) direct-plate count method for the enumeration of Staphylococcus aureus in 6 types of artificially contaminated hard cheese (Asiago, Cheddar, Gruyre, Parmesan, Romano, and Swiss). Five different samples of each cheese type were inoculated with S. aureus (ATCC 25923) to achieve low, medium, and high inoculum levels. S. aureus was enumerated by the Petrifilm and BAM methods, and the results were compared. Multivariate analysis of variance revealed no significant differences (P <0.05) between the 2 methods. The Petrifilm method compared favorably with the BAM procedure. The rapid method was more convenient to use, considerably faster, and less expensive to perform than the BAM method.

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