scholarly journals Comparison of Petrifilm Staph Express Count System with the Bacteriological Analytical Manual Direct-Plating Method for Enumeration of Staphylococcus aureus in Artificially Contaminated Hard Cheese

2008 ◽  
Vol 91 (5) ◽  
pp. 1138-1141 ◽  
Author(s):  
Willis M Fedio ◽  
Chitra N Wendakoon ◽  
Ruben Zapata ◽  
Christina Carrillo ◽  
Paul Browning
Keyword(s):  
Staphylococcus Aureus ◽  
Multivariate Analysis ◽  
Rapid Method ◽  
Analysis Of Variance ◽  
Plate Count ◽  
Direct Plating ◽  
Plating Method ◽  
Plate Count Method ◽  
The U.S ◽  
Hard Cheese

Abstract The 3M Petrifilm Staph Express Count System was compared with the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) direct-plate count method for the enumeration of Staphylococcus aureus in 6 types of artificially contaminated hard cheese (Asiago, Cheddar, Gruyre, Parmesan, Romano, and Swiss). Five different samples of each cheese type were inoculated with S. aureus (ATCC 25923) to achieve low, medium, and high inoculum levels. S. aureus was enumerated by the Petrifilm and BAM methods, and the results were compared. Multivariate analysis of variance revealed no significant differences (P <0.05) between the 2 methods. The Petrifilm method compared favorably with the BAM procedure. The rapid method was more convenient to use, considerably faster, and less expensive to perform than the BAM method.

Isolation and Enumeration of Staphylococcus aureus: Review and Recommendations for Revision of AOAC Method

1971 ◽  
Vol 54 (3) ◽  
pp. 732-735
Author(s):  
E F Baer
Keyword(s):  
Staphylococcus Aureus ◽  
Present Method ◽  
Collaborative Study ◽  
Direct Plating ◽  
Alternative Method ◽  
Plating Method ◽  
Aoac Method ◽  
Aoac Official

Abstract The genesis of the present AOAC official first action method for isolation and enumeration of Staphylococcus is reviewed and information is supplied to support recommendations for revision of the method. It is recommended that the present method be revised by deleting instructions for preparation and use of Vogel and Johnson agar and substituting instructions for preparation and use of Baird-Parker medium. It is also recommended that a direct plating method be provided as an alternative method and that the required collaborative study of the alternative method be conducted. The recommendations have been accepted by the Association.

Precision Parameters for the FDA Shellfish Laboratory Quality Assurance Program for 1973–1989

1995 ◽  
Vol 58 (6) ◽  
pp. 648-650
Author(s):  
JAMES T. PEELER ◽  
THOMAS E. GRAHAM ◽  
LARRY J. MATURIN
Keyword(s):  
Staphylococcus Aureus ◽  
Quality Assurance ◽  
Fecal Coliform ◽  
Quality Assurance Program ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Standard Plate Count ◽  
Standard Plate ◽  
The U.S

Precision parameters from four microbiological analytical methods (coliform most probable number [MPN], fecal coliform MPN, Staphylococcus aureus plate count and standard plate count) were computed for the Shellfish Quality Assurance Program of the U.S. Food and Drug Administration (FDA). The pooled reproducibility variance (SR2) for the four methods from 1973 to 1989 were 0.0778, 0.1181, 0.0137, and 0.0087, respectively.

Direct Plating Method for Enumeration of Staphylococcus aureus: Collaborative Study

1975 ◽  
Vol 58 (6) ◽  
pp. 1154-1158
Author(s):  
Edward F Baer ◽  
James W Messer ◽  
James E Leslie ◽  
James T Peeler
Keyword(s):  
Staphylococcus Aureus ◽  
Sodium Chloride ◽  
Collaborative Study ◽  
General Purpose ◽  
Processed Foods ◽  
Food Ingredients ◽  
Direct Plating ◽  
Coefficients Of Variation ◽  
Plating Method ◽  
Aoac Official

Abstract Considerable evidence has been published regarding the adverse effect of sodium chloride on physiologically impaired cells of Staphylococcus aureus, such as are to be expected in processed foods. A direct plating method for enumeration of S. aureus eliminating the use of sodium chloride was devised and subjected to collaborative study by 16 analysts. Results obtained by the direct plating method were compared to those obtained by the AOAC official first action method (46.036–46.040). Participating analysts examined duplicate samples at population levels of 91, 34, and 20 S. aureus/g. Coefficients of variation among analysts were considerably lower for the direct plating method (31, 81, and 48%, respectively) than for method 46.040 (59, 156, and 150%, respectively) at all 3 population levels. High coefficients of variation for the direct plating method at 2 of the 3 levels were due principally to low populations of S. aureus. The direct plating method has been adopted as official first action for general purpose use and use of method 46.036–46.040 has been restricted to raw food ingredients and nonprocessed foods.

Comparative Efficiency of Two Methods and Two Plating Media for Isolation of Staphylococcus aureus from Foods

1971 ◽  
Vol 54 (3) ◽  
pp. 728-731
Author(s):  
Joseph L Tardio ◽  
Edward F Baer
Keyword(s):  
Staphylococcus Aureus ◽  
International Committee ◽  
Direct Plating ◽  
Comparative Efficiency ◽  
Plating Method ◽  
Aoac Method ◽  
Aoac Official

Abstract Modifications of 2 methods were compared for determination of staphylococci in foods as recommended by the International Committee on Microbiological Specifications for Foods. One method was a direct plating procedure using Baird-Parker (BP) medium. The second method was the current AOAC official first action method for isolation of Staphylococcus aureus from foods (41.018). The efficiency of Vogel and Johnson (VJ) agar and BP as plating media for the AOAC method was also compared. The presence of S. aureus (positive samples) was detected more often by the AOAC method than by the direct plating method. The use of BP as a plating medium in the AOAC method resulted in detection of 25% more positive samples than were detected with VJ agar.

scholarly journals POTENSI EKSTRAK JAHE MERAH (Zingiberaceae officinale rosc)SEBAGAI ANTIMIKROBA DAN PENINGKAT KUALITAS SPERMATOZOA MENCIT YANG DIINFEKSI BAKTERI Staphylococcus aureus SECARA INTRA URETHRA

WAHANA ◽  
2017 ◽  
Vol 69 (2) ◽  
pp. 1-7
Author(s):  
Ersanto Ersanto ◽  
Sukarjati Sukarjati
Keyword(s):  
Staphylococcus Aureus ◽  
Negative Control Group ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Ginger Extract ◽  
Laboratory Rats ◽  
Plate Count Method ◽  
Red Ginger

Red Ginger (Zingiberaceae officinale rosc) is known to be used as an anti-microbial andenhancing the quality of spermatozoa. This study aims to demonstrate of the red ginger extract(Zingiberaceae officinale rosc)potential as an antimicrobial and the quality enhancer of spermatozoain laboratory rats injected by Staphylococcus aureus to its urethra. The red ginger was extracted byethanol. The sample of this research was the spermatozoa of 30 mice that were injected byStaphylococcus aureus to its urethra. Potential test of red ginger extract on the laboratory ratsconducted by observing the spermatozoa’s motility, viability, morphology, the spermatozoa’sconcentration and the amount of spermatozoa leukocyte in the laboratory rats after the administrationof the red ginger extract for 35 days under the microscope. Antimicrobial activity test onStaphylococcus aureus was done by culturing the spermatozoa of laboratory rats (in vivo) afteradministering the red ginger extract for 35 days with total plate count method. The result of the studyshowed that there were differences between negative control group of laboratory rats and positivecontrol group of laboratory rats (laboratory rats injected with Staphylococcus aureus intra urethra)motility (p = 0.000), viability (p = 0.000), morphology (p = 0.000), concentration (p = 0.000), and theamount of leukocyte (p = 0.000). Whereas on the calculation of red ginger extract bacterial coloniesgive the significant effects p <0.05 on the growth of S. aureus. Based on the results of this study, it canbe conclude that the red ginger has potential as an antimicrobial and it also can improve the quality ofspermatozoa in laboratory rats infected with S. aureus through its urethra.

Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli

1988 ◽  
Vol 51 (5) ◽  
pp. 402-404 ◽  
Author(s):  
ELON W. FRAMPTON ◽  
LAWRENCE RESTAINO ◽  
NANCY BLASZKO
Keyword(s):  
Escherichia Coli ◽  
Agar Medium ◽  
Plate Count ◽  
Light Illumination ◽  
Fluorogenic Substrate ◽  
Direct Plating ◽  
E Coli ◽  
Glucuronidase Activity ◽  
Plate Count Agar ◽  
Plating Method

A 24-h direct plating method for Escherichia coli using Peptone-Tergitol agar was used to compare the effectiveness of the chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC) with the fluorogenic substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) for β-glucuronidase activity. Values obtained for enumeration of two strains of E. coli recovered from artificially inoculated raw minced chicken (i.e., plating efficiencies on the inoculum, cells per g, and recovery percentages elated to those on Plate Count Agar) indicate that X-GLUC at 50 μg/ml was as effective as MUG in an agar medium. Unlike MUG, X-GLUC does not require ultraviolet light illumination, and the color reaction produced remains localized in the positive colonies.

scholarly journals Comparison of an Automated Technique TEMPO with Direct Plate Count Method for the Enumeration of Staphylococcus aureus

2013 ◽  
Vol 28 (3) ◽  
pp. 252-257
Author(s):  
Yong Sun Cho ◽  
Da-Yeon Lee ◽  
Joo-Young Lee ◽  
Hae-Jin Wang ◽  
Dong-Bin Shin
Keyword(s):  
Staphylococcus Aureus ◽  
Plate Count ◽  
Count Method ◽  
Plate Count Method ◽  
Automated Technique

scholarly journals Antibacterial Activity and Mechanism of Action of the Silver Ion in Staphylococcus aureus and Escherichia coli

2008 ◽  
Vol 74 (7) ◽  
pp. 2171-2178 ◽  
Author(s):  
Woo Kyung Jung ◽  
Hye Cheong Koo ◽  
Ki Woo Kim ◽  
Sook Shin ◽  
So Hyun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Mechanism Of Action ◽  
Antibacterial Effect ◽  
Silver Ion ◽  
Plate Count ◽  
E Coli ◽  
Count Method ◽  
Plate Count Method ◽  
Conventional Plate

ABSTRACT The antibacterial effect and mechanism of action of a silver ion solution that was electrically generated were investigated for Staphylococcus aureus and Escherichia coli by analyzing the growth, morphology, and ultrastructure of the bacterial cells following treatment with the silver ion solution. Bacteria were exposed to the silver ion solution for various lengths of time, and the antibacterial effect of the solution was tested using the conventional plate count method and flow cytometric (FC) analysis. Reductions of more than 5 log10 CFU/ml of both S. aureus and E. coli bacteria were confirmed after 90 min of treatment with the silver ion solution. Significant reduction of S. aureus and E. coli cells was also observed by FC analysis; however, the reduction rate determined by FC analysis was less than that determined by the conventional plate count method. These differences may be attributed to the presence of bacteria in an active but nonculturable (ABNC) state after treatment with the silver ion solution. Transmission electron microscopy showed considerable changes in the bacterial cell membranes upon silver ion treatment, which might be the cause or consequence of cell death. In conclusion, the results of the present study suggest that silver ions may cause S. aureus and E. coli bacteria to reach an ABNC state and eventually die.

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