A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus
2003 ◽
Vol 47
(3)
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pp. 1120-1124
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ABSTRACT The genes encoding 14α-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.
2005 ◽
Vol 49
(6)
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pp. 2536-2538
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2002 ◽
Vol 184
(3)
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pp. 679-686
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1998 ◽
Vol 180
(21)
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pp. 5612-5618
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1999 ◽
Vol 181
(19)
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pp. 6028-6032
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