scholarly journals Multicenter Study of the Mechanisms of Resistance and Clonal Relationships of Streptococcus agalactiae Isolates Resistant to Macrolides, Lincosamides, and Ketolides in Spain

2005 ◽  
Vol 49 (6) ◽  
pp. 2525-2527 ◽  
Author(s):  
J. J. Gonzalez ◽  
A. Andreu
Keyword(s):  
Pregnant Women ◽  
Multicenter Study ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Clonal Diversity ◽  
Mechanisms Of Resistance ◽  
Resistant Group ◽  
Group B

ABSTRACT Macrolide, lincosamide, and ketolide mechanisms of resistance and clonal relationships were characterized in a collection of 79 resistant group B streptococcus isolates obtained from neonates or pregnant women. The erm(B), erm(TR), and mef(A) genes were present in 62%, 30.4%, and 3.8% of the isolates, respectively. There was considerable clonal diversity among them.

Correlates of Antibiotic-Resistant Group B Streptococcus Isolated From Pregnant Women

2003 ◽  
Vol 101 (1) ◽  
pp. 74-79 ◽  
Author(s):  
Shannon D. Manning ◽  
Betsy Foxman ◽  
Carl L. Pierson ◽  
Patricia Tallman ◽  
Carol J. Baker ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Group B Streptococcus ◽  
Antibiotic Resistant ◽  
Resistant Group ◽  
Group B

scholarly journals Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran

2020 ◽  
Author(s):  
Leila Goudarzi ◽  
Mohammad Bagher Khalili ◽  
Mahmood Vakili ◽  
Maryam Sadeh
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Specific Pcr ◽  
Pcr Method ◽  
Group B ◽  
Tehran Area

Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.

scholarly journals Multicenter Study of a Rapid Molecular-Based Assay for the Diagnosis of Group B Streptococcus Colonization in Pregnant Women

2004 ◽  
Vol 39 (8) ◽  
pp. 1129-1135 ◽  
Author(s):  
H. D. Davies ◽  
M. A. Miller ◽  
S. Faro ◽  
D. Gregson ◽  
S. C. Kehl ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Multicenter Study ◽  
Group B Streptococcus ◽  
Group B

Detection of Streptococcus agalactiae by immunochromatography with group B streptococcus - specific surface immunogenic protein in pregnant women

2017 ◽  
Vol 23 (10) ◽  
pp. 678-682 ◽  
Author(s):  
Yoko Takayama ◽  
Hidehito Matsui ◽  
Yuzuru Adachi ◽  
Shin Nihonyanagi ◽  
Tatsuhiko Wada ◽  
...  
Keyword(s):  
Specific Surface ◽  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Immunogenic Protein ◽  
Group B

scholarly journals Cellular Management of Zinc in Group B Streptococcus Supports Bacterial Resistance against Metal Intoxication and Promotes Disseminated Infection

mSphere ◽  
2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Matthew J. Sullivan ◽  
Kelvin G. K. Goh ◽  
Glen C. Ulett
Keyword(s):  
Streptococcus Agalactiae ◽  
Bacterial Resistance ◽  
Group B Streptococcus ◽  
Systemic Infection ◽  
Metal Stress ◽  
Mechanisms Of Resistance ◽  
Content Type ◽  
Group B ◽  
Zn Stress

ABSTRACT Zinc is an essential trace element for normal bacterial physiology but, divergently, can intoxicate bacteria at high concentrations. Here, we define the molecular systems for Zn detoxification in Streptococcus agalactiae, also known as group B streptococcus, and examine the effects of resistance to Zn stress on virulence. We compared the growth of wild-type bacteria and mutants deleted for the Zn exporter, czcD, and the response regulator, sczA, using Zn-stress conditions in vitro. Macrophage antibiotic protection assays and a mouse model of disseminated infection were used to assess virulence. Global bacterial transcriptional responses to Zn stress were defined by RNA sequencing and quantitative reverse transcription-PCR. czcD and sczA enabled S. agalactiae to survive Zn stress, with the putative CzcD efflux system activated by SczA. Additional genes activated in response to Zn stress encompassed divalent cation transporters that contribute to regulation of Mn and Fe homeostasis. In vivo, the czcD-sczA Zn management axis supported virulence in the blood, heart, liver, and bladder. Additionally, several genes not previously linked to Zn stress in any bacterium, including, most notably, arcA for arginine deamination, also mediated resistance to Zn stress, representing a novel molecular mechanism of bacterial resistance to metal intoxication. Taken together, these findings show that S. agalactiae responds to Zn stress by sczA regulation of czcD, with additional novel mechanisms of resistance supported by arcA, encoding arginine deaminase. Cellular management of Zn stress in S. agalactiae supports virulence by facilitating bacterial survival in the host during systemic infection. IMPORTANCE Streptococcus agalactiae, also known as group B streptococcus, is an opportunistic pathogen that causes various diseases in humans and animals. This bacterium has genetic systems that enable zinc detoxification in environments of metal stress, but these systems remain largely undefined. Using a combination of genomic, genetic, and cellular assays, we show that this pathogen controls Zn export through CzcD to manage Zn stress and utilizes a system of arginine deamination never previously linked to metal stress responses in bacteria to survive metal intoxication. We show that these systems are crucial for survival of S. agalactiae in vitro during Zn stress and also enhance virulence during systemic infection in mice. These discoveries establish new molecular mechanisms of resistance to metal intoxication in bacteria; we suggest these mechanisms operate in other bacteria as a way to sustain microbial survival under conditions of metal stress, including in host environments.

scholarly journals Infection and drug resistance of Streptococcus agalactiae among perinatal pregnant women in Xinjiang

2021 ◽  
Author(s):  
Shuli Guo ◽  
Xiandao Luo ◽  
Haiying Jia ◽  
Xiuhui Pang ◽  
Changmin Wang ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Target Site ◽  
Macrolide Resistance ◽  
Diffusion Method ◽  
Resistance Phenotype ◽  
Ermb Gene ◽  
Group B

Abstract Background: Group B streptococcus (Streptococcus agalactiae) is one of the most common pathogens causing meningitis, bacteremia and pneumonia. The drug resistance mechanisms of group B streptococcus in different countries and regions also show regional differences.Method: The study population was comprised of 1877 pregnant women of 34-38 weeks who underwent prenatal examination in the gynecology and obstetrics outpatient clinic of Xinjiang People's Hospital, between January 1, 2019 and January 31, 2020. Clinic specimens were collected and identified by the API bacteria Rapid Identification card for the downstream group B Streptococcus (Streptococcus agalactiae) isolation. Drug susceptibility of the Streptococcus agalactiae isolated was detected by Kirby-Bauer disk diffusion method. Macrolide–lincosamide–streptogramin B (MLSB) resistance was determined by D test. Real-time quantitative polymerase chain reaction and Gene sequencing was performed for the resistance genes ermA, ermB, mreA, erm (47), mefA/E and Lin B.Results: 149 Streptococcus agalactiae-positive strains were identified by clinical isolation, with a positive rate of 7.94%. Group B Streptococcus showed 100% susceptibility to linezolid, penicillin, vancomycin, meropenem, ampicillin, ceftriaxone, 44.97%, 35.57%, 56.38% and 29.53% susceptibility to levofloxacin, erythromycin, tetracycline and clindamycin, respectively. Among the 149 isolates, 127 strains showed macrolide resistance phenotype. The detection rate of intrinsic resistance phenotype (cMLS) was 40.94% (59/127), active efflux resistance phenotype (MS) 9.45% (12/127), and induced resistance phenotype (iMLS) 22.83% (29/127).Conclusion: The ermB gene-mediated 50s ribosome target site change co-existing with mreA gene for macrolide resistance efflux may play a major role in the mechanism of Streptococcus agalactiae resistance macrolide resistance of in perinatal women in Xinjiang. The change of 50s ribosomal target site mediated by ermB gene may be the main reason for drug cross-resistance.

scholarly journals Oxytocin in Induction of Labor in Pregnancy with Group B Streptococcal Portage

2020 ◽  
Vol 25 (3) ◽  
pp. 42-47
Author(s):  
Mihaela Corina Radu ◽  
Anca Irina Dumitrescu ◽  
Adrian Calin Boeru ◽  
Loredana Sabina Cornelia Manolescu ◽  
Oana Roxana Dumitrescu ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Perinatal Transmission ◽  
Induction Of Labor ◽  
Morbidity And Mortality ◽  
Perinatal Morbidity ◽  
Induced Labor ◽  
Group B ◽  
In Pregnancy

AbstractIn the last two decades, group B streptococcus (GBS) infection has established itself as a major cause of perinatal morbidity and mortality. The purpose of this study is to identify if the electively induced labor with oxytocin in women with positive cultures of Streptococcus agalactiae, namely the group B streptococcus (GBS), helps the mother and fetus and decreases the risk associated with perinatal transmission of GBS compared with the spontaneous labor. Results associated with induction of labor with oxytocin compared with spontaneous labor in pregnant women who have GBS - positive cervical cultures, are also used to determine whether induction of labor decreases the risk of complications from GBS infection.

scholarly journals Cellular management of Zinc in group B Streptococcus supports bacterial resistance against metal intoxication and promotes disseminated infection

2021 ◽  
Author(s):  
Matthew J. Sullivan ◽  
Kelvin G. K. Goh ◽  
Glen C. Ulett
Keyword(s):  
Streptococcus Agalactiae ◽  
Bacterial Resistance ◽  
Group B Streptococcus ◽  
Systemic Infection ◽  
Metal Stress ◽  
Mechanisms Of Resistance ◽  
Disseminated Infection ◽  
Group B ◽  
Zn Stress

AbstractZinc (Zn) is an essential trace element for normal bacterial physiology but divergently, can intoxicate bacteria at high concentrations. Here, we define the molecular systems for Zn detoxification in Streptococcus agalactiae, also known as group B Streptococcus, and examine the effects of resistance to Zn stress on virulence. We compared the growth of wild-type bacteria and mutants deleted for the Zn exporter, czcD, and the response regulator, sczA, using Zn-stress conditions in vitro. Macrophage antibiotic protection assays and a mouse model of disseminated infection were used to assess virulence. Global bacterial transcriptional responses to Zn stress were defined by RNA-sequencing and qRTPCR. czcD and sczA enabled S. agalactiae to survive Zn stress, with the putative CzcD efflux system activated by SczA. Additional genes activated in response to Zn stress encompassed divalent cation transporters that contribute to regulation of Mn and Fe homeostasis. In vivo, the czcD-sczA Zn-management axis supported virulence in the blood, heart, liver and bladder. Additionally, several genes not previously linked to Zn stress in any bacterium, including most notably, arcA for arginine deamination also mediated resistance to Zn stress; representing a novel molecular mechanism of bacterial resistance to metal intoxication. Taken together, these findings show that S. agalactiae responds to Zn stress by sczA regulation of czcD, with additional novel mechanisms of resistance supported by arcA, encoding arginine deaminase. Cellular management of Zn stress in S. agalactiae supports virulence by facilitating bacterial survival in the host during systemic infection.Importance StatementStreptococcus agalactiae, also known as group B streptococcus, is an opportunistic pathogen that causes various diseases in humans and animals. This bacterium has genetic systems that enable Zinc (Zn) detoxification in environments of metal stress, but these systems remain largely undefined. Using a combination of genomic, genetic and cellular assays we show that this pathogen controls Zn export through CzcD to manage Zn stress, and utilizes a system of arginine deamination never previously linked to metal stress responses in bacteria to survive metal intoxication. We show that these systems are crucial for survival of S. agalactiae in vitro during Zn stress and also enhance virulence during systemic infection in mice. These discoveries establish new molecular mechanisms of resistance to metal intoxication in bacteria; we suggest these mechanisms are likely to operate in other bacteria as a way to sustain microbial survival in conditions of metal stress, including in host environments.

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