High-efficiency genome editing based on endogenous CRISPR-Cas system enhances cell growth and lactic acid production in Pediococcus acidilactici

2021 ◽  
Author(s):  
Ling Liu ◽  
Danlu Yang ◽  
Zhiyu Zhang ◽  
Tao Liu ◽  
Guoquan Hu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Growth ◽  
Genome Editing ◽  
Acid Production ◽  
Gene Deletion ◽  
High Efficiency ◽  
Lactic Acid Production ◽  
Pediococcus Acidilactici ◽  
Acid Fermentation ◽  
Genetic Manipulations

Pediococcus acidilactici is commonly used for pediocin production and lactic acid fermentation. However, high-efficiency genome editing tool is unavailable for this species. In this study, we constructed the endogenous subtype II-A CRISPR-Cas system-based genome interference plasmids which carried a “Repeat-Spacer-Repeat” cassette in the pMG36e shuttle vector. These plasmids exhibited self-interference activities in P. acidilactici LA412. Then, the genome-editing plasmids were constructed by cloning the upstream/downstream donor DNA into the corresponding interference plasmids to exert high-efficiency markerless gene deletion, gene integration, and point mutation in P. acidilactici LA412. We found that endogenous CRISPR-mediated depletion of the native plasmids enhanced the cell growth, and integration of a L-lactate dehydrogenase gene into the chromosome both enhanced cell growth and lactic acid production. IMPORTANCE A rapid and precise genome editing tool will promote the practical application of Pediococcus acidilactici , one type of lactic acid bacteria with excellent stress tolerance and probiotic characteristics. This study established a high-efficiency endogenous CRISPR-Cas system-based genome editing tool for P. acidilactici and achieved different genetic manipulations, including gene deletion, gene insertion, mononucleotide mutation, and endogenous plasmid depletion. The engineered strain edited by this tool showed significant advantages in cell growth and lactic acid fermentation. Therefore, our tool can satisfy the requirements for genetic manipulations of P. acidilactici , thus making it a sophisticated chassis species for synthetic biology and bioindustry.

Direct lactic acid fermentation: Focus on simultaneous saccharification and lactic acid production

2009 ◽  
Vol 27 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Rojan P. John ◽  
Anisha G.S. ◽  
K. Madhavan Nampoothiri ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation ◽  
Simultaneous Saccharification

scholarly journals Evaluation of conditions for fermentation of fish offal

1995 ◽  
Vol 4 (1) ◽  
pp. 11-17
Author(s):  
T. Mikael Lassén
Keyword(s):  
Lactic Acid ◽  
Redox Potential ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Period ◽  
Growth Conditions ◽  
Inoculum Size ◽  
Reliable Estimate ◽  
Small Scale ◽  
Acid Fermentation

Conditions for the lactic acid fermentation of fish offal were evaluated regarding the effect of substrate concentration (2, 5 and 10% dextrose), preacidification with lactic acid (initial pH of 6.8, to 6.5 or 6.0), and inoculum size of Lactobacillus plantarum (107 , 108 and 109 colony forming units (cfu)/g). pH and lactic acid production were monitored during a two-week storage period. A small-scale silo for fermenting fish offal was also constructed, and measurement of redox potential was evaluated as a means to estimate bacterial growth conditions. The most favourable conditions for fermentation, manifested by a low and stable pH and high lactic acid production, were achieved with an inoculum size of 108 cfu/g and 5% dextrose. Preacidification did not affect final pH. Redox potential was shown to give a reliable estimate of growth conditions for bacteria under anaerobic conditions by rapidly falling to below -550mV in silage with a high lactic acid concentration.

L(+)-Lactic Acid Production in Plaque from Orthodontic Appliances Retained with Glass Ionomer Cement

1994 ◽  
Vol 21 (1) ◽  
pp. 23-26 ◽  
Author(s):  
A. Hallgren ◽  
A. Oliveby ◽  
S. Twetman
Keyword(s):  
Lactic Acid ◽  
Incubation Period ◽  
Acid Production ◽  
Orthodontic Treatment ◽  
Lactic Acid Production ◽  
Glass Ionomer Cement ◽  
Orthodontic Appliances ◽  
Glass Ionomer ◽  
Acid Fermentation ◽  
Ionomer Cement

The lactic acid production in suspensions of plaque collected adjacent to orthodontic brackets retained with a glass ionomer cement (GIC), or a resin-based composite was investigated using a split-mouth technique. Forty-eight-hour-old plaque was collected at 3, 8, and 28 days, and 3 months after the onset of orthodontic treatment. Acid fermentation was induced by glucose and the L(+)-lactic acid concentrations were determined enzymatically after a 30-minute incubation period. Significantly (P<0·05) lower levels of lactic acid were found in plaque from GIC-retained brackets compared with the composite controls at the 28 days and 3 months sampling occasions, respectively. The results suggest that the use of GIC as a bonding agent in orthodontics can be beneficial for patients assessed at risk of caries development.

scholarly journals Effect of β-glycosidase supplementation on vinasse saccharification and L-lactic acid fermentation

BioResources ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 1379-1389
Author(s):  
Yingnan Cao ◽  
Juan Wang ◽  
Qunhui Wang ◽  
Jianguo Liu ◽  
Tingxi Liu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lignocellulosic Biomass ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Glucose Production ◽  
Utilization Efficiency ◽  
Sugar Accumulation ◽  
Biomass Hydrolysis ◽  
Acid Fermentation ◽  
Separate Hydrolysis And Fermentation

Efficient pretreatment and enzymatic hydrolysis is critical to achieve effective utilization of lignocellulosic biomass. In this study, the cellulase composition for lignocellulosic biomass hydrolysis was strategically optimized to improve the efficiency of vinasse saccharification and thus enhance L-lactic acid production. The results showed that the supplementation of β-glycosidase (BG) increased sugar production, and the glucose concentration exceeded cellobiose concentration after 48 h of hydrolysis. These results suggested that the addition of BG aided the hydrolysis of cellobiose and reduced the inhibitory effects caused by sugar accumulation. After 72 h to 96 h of hydrolysis, the BG supplementation improved cellobiose and glucose production by 25.7% and 27.4%, respectively. The effect of BG supplementation on L-lactic acid production during the fermentation of microwave-alkali pretreated vinasse was also investigated. Here, the L-lactic acid production from simultaneous saccharification and fermentation (SSF) with the addition of BG was 20.8% higher than that without BG addition, and was also 37.0% higher than production from separate hydrolysis and fermentation with BG addition. These results indicated the utilization efficiency of lignocellulosic biomass for L-lactic acid production could be enhanced by supplementation of BG in SSF.

scholarly journals A study on Lactic Acid fermentation of Sugar Palm (Arenga Pinnata) sap by Lactobacillus Casei

2014 ◽  
Vol 17 (2) ◽  
pp. 65-71
Author(s):  
Nhat Minh Dang ◽  
Trung Quang Nguyen
Keyword(s):  
Lactic Acid ◽  
Calcium Carbonate ◽  
Ammonium Sulphate ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Microbial Culture ◽  
Acid Fermentation ◽  
Lower Ability

This study aimed to evaluate the effect of various factors on the yield of lactic acid fermentation using sap from sugar palm and Lactobacillus casei. The sugar palm sap after harvesting, pretreatment was added with ammonium sulphate, calcium carbonate and microbial culture at density of 109 cells/ml and let fermented for acid lactic production. The results of the experiments showed that the culture size, amount of added ammonium sulphate and calcium carbonate had significant effect on lactic acid production. The most appropriate parameters determined were culture size of 5%, ammonium sulphate of 3.0 g/l and calcium carbonate of 4.0 g/l. Meanwhile, the optimum period of fermentation was 100 h, which gave the yield of lactic acid production of 22.30 g. Lactobacillus casei was considered to have lower ability to effectively produce lactic acid from sugar palm sap compared to Lactobacillus plantarum

scholarly journals Nutrient value of fish manure waste on lactic acid fermentation by Lactobacillus pentosus

RSC Advances ◽  
2018 ◽  
Vol 8 (55) ◽  
pp. 31267-31274 ◽  
Author(s):  
Suan Shi ◽  
Jing Li ◽  
Wenjian Guan ◽  
David Blersch
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Lactic Acid Production ◽  
Lactic Acid Fermentation ◽  
Nutrient Source ◽  
Acid Fermentation ◽  
Lactobacillus Pentosus ◽  
Nutrient Value ◽  
Simultaneous Saccharification

Fish manure wastes are an effective nutrient source for lactic acid production using simultaneous saccharification and fermentation.

scholarly journals Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer

2017 ◽  
Vol 66 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Guoping Lv ◽  
Chengchuan Che ◽  
Li Li ◽  
Shujing Xu ◽  
Wanyi Guan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Fermentation Process ◽  
Specific Activity ◽  
Lactic Acid Production ◽  
Optical Purity ◽  
Ammonium Ion ◽  
High Concentration ◽  
Acid Fermentation ◽  
High Optical Purity

The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/l of D-lactic acid production and 0.89 g/g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/l betaine in the simple batch fermentation. The addition of betaine was experimentally proven to protect cell at high concentration of ammonium ion, increase the D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid.

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