scholarly journals Respiration Response Imaging for Real-Time Detection of Microbial Function at the Single-Cell Level

2010 ◽  
Vol 77 (1) ◽  
pp. 67-72 ◽  
Author(s):  
M. C. Konopka ◽  
T. J. Strovas ◽  
David S. Ojala ◽  
L. Chistoserdova ◽  
M. E. Lidstrom ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Cell ◽  
Real Time ◽  
Microbial Population ◽  
Single Cell Level ◽  
Cell Level ◽  
Microbial Cells ◽  
Microbial Function ◽  
Functional Profiling ◽  
Natural Communities

ABSTRACTThe ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C1substrates, at a single-cell level. We demonstrate that RRI could be used for the investigation of heterogeneity of a single microbial population or for functional profiling of microbial cells from complex environmental communities, such as freshwater lake sediment.

scholarly journals Investigating DNA Replication in Escherichia Coli on the Single Cell Level Utilizing Microfluidics and Single-Molecule Fluorescence Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 368a-369a
Author(s):  
Charl Moolman ◽  
Sriram T. Krishnan ◽  
Jacob W.K. Kerssemakers ◽  
Susanne Hage ◽  
Rodrigo Reyes-Lamothe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Molecule ◽  
Single Cell Level ◽  
Single Molecule Fluorescence ◽  
Cell Level ◽  
Single Molecule Fluorescence Microscopy

scholarly journals Detection of PLGA-based nanoparticles at a single-cell level by synchrotron radiation FTIR spectromicroscopy and correlation with X-ray fluorescence microscopy

2014 ◽  
pp. 2791 ◽  
Author(s):  
Giovanni Maria Severini ◽  
Lorella Pascolo ◽  
Barbara Bortot ◽  
Nuria Benseny-Cases ◽  
Alessandra Gianoncelli ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Fluorescence Microscopy ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
X Ray

Measuring bacterial adaptation dynamics at the single-cell level using a microfluidic chemostat and time-lapse fluorescence microscopy

The Analyst ◽  
2014 ◽  
Vol 139 (20) ◽  
pp. 5254-5262 ◽  
Author(s):  
Zhicheng Long ◽  
Anne Olliver ◽  
Elisa Brambilla ◽  
Bianca Sclavi ◽  
Marco Cosentino Lagomarsino ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Cell ◽  
Time Lapse ◽  
Single Cell Level ◽  
Cell Level ◽  
Gfp Expression ◽  
Bacterial Adaptation ◽  
E Coli ◽  
Cell Dimensions

We grewE. coliin a microfluidic chemostat and monitored the dynamics of cell dimensions and reporter GFP expression in individual cells during nutritional upshift or downshift.

Measurement of Molecular Mobility in Mammalian Cells

2004 ◽  
Author(s):  
Alptekin Aksan ◽  
Mehmet Toner
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Chemical Reaction ◽  
Molecular Mobility ◽  
Mammalian Cells ◽  
Free Water ◽  
Single Cell Level ◽  
Extracellular Water ◽  
Cell Level

Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

scholarly journals Method for real-time monitoring of protein degradation at the single cell level

BioTechniques ◽  
2007 ◽  
Vol 42 (4) ◽  
pp. 446-450 ◽  
Author(s):  
Lijuan Zhang ◽  
Nadya G. Gurskaya ◽  
Ekaterina M. Merzlyak ◽  
Dmitry B. Staroverov ◽  
Nikolay N. Mudrik ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level
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