Simple Method for Repurification of Endotoxins for Biological Use
2007 ◽
Vol 73
(6)
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pp. 1803-1808
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Keyword(s):
ABSTRACT A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry analysis confirmed the integrity of the purified LPSs. The purified products did not activate Toll-like receptor 2 (TLR2), nuclear oligomerization domain 1 (NOD1), or NOD2 but did activate TLR4. Applied to different lipopolysaccharides, the method also improved their mass spectra, thus facilitating their structural analysis.
2009 ◽
Vol 75
(14)
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pp. 4661-4667
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2007 ◽
Vol 73
(7)
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pp. 2247-2250
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2009 ◽
Vol 72
(12)
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pp. 2524-2529
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2011 ◽
Vol 57
(12)
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pp. 993-1001
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2010 ◽
Vol 76
(22)
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pp. 7536-7540
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