Quantitative Trait Locus Mapping of Yield-Related Components and Oligogenic Control of the Cap Color of the Button Mushroom, Agaricus bisporus

ABSTRACTAs in other crops, yield is an important trait to be selected for in edible mushrooms, but its inheritance is poorly understood. Therefore, we have investigated the complex genetic architecture of yield-related traits inAgaricus bisporusthrough the mapping of quantitative trait loci (QTL), using second-generation hybrid progeny derived from a cross between a wild strain and a commercial cultivar. Yield, average weight per mushroom, number of fruiting bodies per m2, earliness, and cap color were evaluated in two independent experiments. A total of 23 QTL were detected for 7 yield-related traits. These QTL together explained between 21% (two-flushes yield) and 59% (earliness) of the phenotypic variation. Fifteen QTL (65%) were consistent between the two experiments. Four regions underlying significant QTL controlling yield, average weight, and number were detected on linkage groups II, III, IV, and X, suggesting a pleiotropic effect or tight linkage. Up to six QTL were identified for earliness. ThePPC1locus, together with two additional genomic regions, explained up to 90% of the phenotypic variation of the cap color. Alleles from the wild parent showed beneficial effects for some yield traits, suggesting that the wild germ plasm is a valuable source of variation for several agronomic traits. Our results constitute a key step toward marker-assisted selection and provide a solid foundation to go further into the biological mechanisms controlling productive traits in the button mushroom.

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Relationship between Yield Components and Partial Resistance to Lecanicillium fungicola in the Button Mushroom, Agaricus bisporus, Assessed by Quantitative Trait Locus Mapping

Applied and Environmental Microbiology ◽

10.1128/aem.07554-11 ◽

2012 ◽

Vol 78 (7) ◽

pp. 2435-2442 ◽

Cited By ~ 23

Author(s):

Marie Foulongne-Oriol ◽

Anne Rodier ◽

Jean-Michel Savoie

Keyword(s):

Quantitative Trait ◽

Wild Strain ◽

Genomic Region ◽

Yield Component ◽

Phenotypic Variance ◽

Button Mushroom ◽

Content Type ◽

Dry Bubble ◽

Trait Locus ◽

Genomic Regions

ABSTRACTDry bubble, caused byLecanicillium fungicola, is one of the most detrimental diseases affecting button mushroom cultivation. In a previous study, we demonstrated that breeding for resistance to this pathogen is quite challenging due to its quantitative inheritance. A second-generation hybrid progeny derived from an intervarietal cross between a wild strain and a commercial cultivar was characterized forL. fungicolaresistance under artificial inoculation in three independent experiments. Analysis of quantitative trait loci (QTL) was used to determine the locations, numbers, and effects of genomic regions associated with dry-bubble resistance. Four traits related to resistance were analyzed. Two to four QTL were detected per trait, depending on the experiment. Two genomic regions, on linkage group X (LGX) and LGVIII, were consistently detected in the three experiments. The genomic region on LGX was detected for three of the four variables studied. The total phenotypic variance accounted for by all QTL ranged from 19.3% to 42.1% over all traits in all experiments. For most of the QTL, the favorable allele for resistance came from the wild parent, but for some QTL, the allele that contributed to a higher level of resistance was carried by the cultivar. Comparative mapping with QTL for yield-related traits revealed five colocations between resistance and yield component loci, suggesting that the resistance results from both genetic factors and fitness expression. The consequences for mushroom breeding programs are discussed.

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Wpływ substancji zawartych w pieczarkach na organizm człowieka

Medycyna Rodzinna ◽

10.25121/mr.2018.21.2.151 ◽

2018 ◽

Vol 21 (2) ◽

Author(s):

Jacek Majewski ◽

Michał Orylski ◽

Maciej Majewski ◽

Julia Rasała

Keyword(s):

Agaricus Bisporus ◽

Positive Impact ◽

Human Immune System ◽

Button Mushroom ◽

Beneficial Effects ◽

White Button Mushroom ◽

Lower Body Weight ◽

Estrogen Synthesis ◽

Lower Blood ◽

Iga Production

Agaricus bisporus (white button mushroom) contains significant amounts of dietary fibers, microelements and other important compounds. While it is often underrated, numerous studies prove its positive impact on health. Several compounds found in Agaricus bisporus provide beneficial effects on diabetic and cardiovascular diseases and may lower blood glucose, cholesterol and LDL. Those mushrooms are also a potential breast cancer chemopreventive agent since they were proven to suppress aromatase and estrogen synthesis. Therefore, it may be useful in estrogen-dependent breast tumors. What is more, white button mushroom contains low amount of fat and is low calorie. It can be effectively used in diets to lower body weight. Also, compounds found in white button mushroom have impact on human immune system. They lead to increase of IgA production and stimulates lymphocytes by increasing levels of interleukin. Therefore, white button mushroom is not only valuable thanks to its taste but also because of its impact on human health. Properly prepared it can be an important ingredient of everyday meals.

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Sensory, nutritional and quality attributes of sponge cake supplemented with mushroom (agaricus bisporus) powder

Nutrition & Food Science ◽

10.1108/nfs-12-2016-0187 ◽

2017 ◽

Vol 47 (4) ◽

pp. 578-590 ◽

Cited By ~ 4

Author(s):

Bindvi Arora ◽

Shwet Kamal ◽

Ved Prakash Sharma

Keyword(s):

Nutritional Status ◽

Specific Volume ◽

Agaricus Bisporus ◽

Button Mushroom ◽

Content Type ◽

Sponge Cake ◽

White Button Mushroom ◽

Significant Difference ◽

Protein Rich

Purpose The purpose of this paper was to evaluate the quality characteristics of white button mushroom powder enrichment in sponge cake. The developed product can be a promising inclusion in the convenience functional foods and would serve the purpose of improving nutritional status of consumers. Design/methodology/approach Cakes containing graded levels of agaricus bisporus (white button mushroom) powder were prepared by replacing wheat flour with 10, 20, 30, 40 and 50 per cent mushroom powder. The effect of mushroom powder supplementation on physical, nutritive and sensory quality of the cakes was evaluated and analyzed statistically using Agres-Agdata software. Findings Batter density and specific volume of cake samples decreased with increasing levels of mushroom powder in the formulation, which is contrary to the knowledge that they are inversely proportional. For 20 per cent enrichment, batter density and specific volume decreased by 8.23 and 5.79 per cent, respectively. There was no significant difference in individual organoleptic attributes of cake samples up to a level of 20 per cent fortification of mushroom powder, with significant nutrient enrichment (p > 0.05). Originality/value Replacing flour in cakes has been tried with many protein-rich ingredients to improve the nutritional quality of cake although with limiting success when it comes to sensorial acceptability. Mushroom powder was incorporated in the formulation to enrich sponge cakes. The processing technology will also reduce post-harvest losses in mushrooms and improve the nutritional status of society.

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Biofilm Formation and Synthesis of Antimicrobial Compounds by the Biocontrol Agent Bacillus velezensis QST713 in an Agaricus bisporus Compost Micromodel

Applied and Environmental Microbiology ◽

10.1128/aem.00327-19 ◽

2019 ◽

Vol 85 (12) ◽

Cited By ~ 6

Author(s):

Caroline Pandin ◽

Maud Darsonval ◽

Camille Mayeur ◽

Dominique Le Coq ◽

Stéphane Aymerich ◽

...

Keyword(s):

Growth Phase ◽

Biofilm Formation ◽

Agaricus Bisporus ◽

Biocontrol Agent ◽

Exponential Growth Phase ◽

Antimicrobial Compounds ◽

Bacillus Velezensis ◽

Button Mushroom ◽

Content Type ◽

Green Mold

ABSTRACT Bacillus velezensis QST713 is widely used as a biological control agent for crop protection and disease suppression. This strain is used industrially in France for the protection of Agaricus bisporus against Trichoderma aggressivum f. europaeum, which causes green mold disease. The efficacy of this biocontrol process was evaluated in a previous study, yet the mode of its action has not been explored under production conditions. In order to decipher the underlying biocontrol mechanisms for effective biofilm formation by strain QST713 in the compost and for the involvement of antimicrobial compounds, we developed a simplified micromodel for the culture of A. bisporus during its early culture cycle. By using this micromodel system, we studied the transcriptional response of strain QST713 in the presence or absence of A. bisporus and/or T. aggressivum in axenic industrial compost. We report the overexpression of several genes of the biocontrol agent involved in biofilm formation in the compost compared to their expression during growth in broth compost extract either in the exponential growth phase (the epsC, blsA, and tapA genes) or in the stationary growth phase (the tapA gene), while a gene encoding a flagellar protein (hag) was underexpressed. We also report the overexpression of Bacillus velezensis QST713 genes related to surfactin (srfAA) and fengycin (fenA) production in the presence of the fungal pathogen in the compost. IMPORTANCE Biocontrol agents are increasingly used to replace chemical pesticides to prevent crop diseases. In the button mushroom field in France, the use of Bacillus velezensis QST713 as a biocontrol agent against the green mold Trichoderma aggressivum has been shown to be efficient. However, the biocontrol mechanisms effective in the Agaricus bisporus/Trichoderma aggressivum/Bacillus velezensis QST713 pathosystem are still unknown. Our paper focuses on the exploration of the bioprotection mechanisms of the biocontrol agent Bacillus velezensis QST713 during culture of the button mushroom (Agaricus bisporus) in a micromodel culture system to study the specific response of strain QST713 in the presence of T. aggressivum and/or A. bisporus.

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Genome Sequence of Lecanicillium fungicola 150-1, the Causal Agent of Dry Bubble Disease

Microbiology Resource Announcements ◽

10.1128/mra.00340-19 ◽

2019 ◽

Vol 8 (19) ◽

Cited By ~ 1

Author(s):

Alice M. Banks ◽

Farhana Aminuddin ◽

Katherine Williams ◽

Thomas Batstone ◽

Gary L. A. Barker ◽

...

Keyword(s):

Genome Sequence ◽

Agaricus Bisporus ◽

Control Strategies ◽

Causal Agent ◽

Button Mushroom ◽

Content Type ◽

White Button Mushroom ◽

Dry Bubble ◽

Dry Bubble Disease

The fungus Lecanicillium fungicola causes dry bubble disease in the white button mushroom Agaricus bisporus. Control strategies are limited, as both the host and pathogen are fungi, and there is limited understanding of the interactions in this pathosystem.

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