scholarly journals Triacylglycerol Profiles of Tilletia controversa and Tilletia tritici

1993 ◽  
Vol 59 (4) ◽  
pp. 1054-1057 ◽  
Author(s):  
S. E. Beattie ◽  
A. E. Stafford ◽  
A. D. King
Keyword(s):  
Tilletia Tritici ◽  
Tilletia Controversa ◽  
Triacylglycerol Profiles

Low-temperature storage of monosporidial cultures of the wheat bunt fungi

1995 ◽  
Vol 73 (5) ◽  
pp. 758-760 ◽  
Author(s):  
Patricia Loomis ◽  
Hei Leung
Keyword(s):  
Somatic Mutations ◽  
Interspecific Crosses ◽  
Meiotic Segregation ◽  
Serial Transfer ◽  
Tilletia Tritici ◽  
Storage Method ◽  
Low Temperature Storage ◽  
Tilletia Controversa ◽  
Dwarf Bunt ◽  
Temperature Storage

Collections of Tilletia species are commonly maintained in the form of sori harvested from smutted heads. This has been a convenient and effective storage method because teliospores enclosed in a sorus remain viable for over 20 years. However, mycelial cultures derived from germinated teliospores are genetically mixed because of meiotic segregation among sporidia. For the purposes of genetic analysis and establishing strain identity, it is necessary to maintain haploid sporidial cultures that can be readily retrieved for manipulations. We found that sporidial cultures of Tilletia tritici and Tilletia controversa can be preserved for at least 1 year by suspending a mixture of mycelia and sporidia in 15% glycerol and storing at −70 °C. Approximately 80% of the stored cultures were viable after 12 months in storage. Five out of nine monosporidial cultures of T. tritici and T. controversa tested retained their sexual fertility in interspecific crosses. The glycerol storage method has advantages over serial transfer in the maintenance of monosporidial cultures by reducing the accumulation of somatic mutations; however, the technique should be used cautiously until greater infectivity of stored cultures is demonstrated. Key words: common and dwarf bunt fungi, Tilletia controversa, Tilletia tritici.

Discribution and ecophysiological characterics of the fungus Tilletia controversa in Slovakia.

1995 ◽  
Vol 48 (3) ◽  
pp. 207-216
Author(s):  
Peter Paulech ◽  
Cyprián Paulech
Keyword(s):  
Tilletia Controversa

scholarly journals Development of a loop-mediated isothermal amplification assay for the detection of Tilletia controversa based on genome comparison

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Somayyeh Sedaghatjoo ◽  
Monika K. Forster ◽  
Ludwig Niessen ◽  
Petr Karlovsky ◽  
Berta Killermann ◽  
...  
Keyword(s):  
Test Performance ◽  
Genomic Dna ◽  
Lamp Assay ◽  
Fungal Species ◽  
Isothermal Amplification ◽  
Genome Comparison ◽  
Performance Study ◽  
Loop Mediated Isothermal Amplification ◽  
Tilletia Controversa ◽  
Genomic Regions

AbstractTilletia controversa causing dwarf bunt of wheat is a quarantine pathogen in several countries. Therefore, its specific detection is of great phytosanitary importance. Genomic regions routinely used for phylogenetic inferences lack suitable polymorphisms for the development of species-specific markers. We therefore compared 21 genomes of six Tilletia species to identify DNA regions that were unique and conserved in all T. controversa isolates and had no or limited homology to other Tilletia species. A loop-mediated isothermal amplification (LAMP) assay for T. controversa was developed based on one of these DNA regions. The specificity of the assay was verified using 223 fungal samples comprising 43 fungal species including 11 Tilletia species, in particular 39 specimens of T. controversa, 92 of T. caries and 40 of T. laevis, respectively. The assay specifically amplified genomic DNA of T. controversa from pure cultures and teliospores. Only Tilletia trabutii generated false positive signals. The detection limit of the LAMP assay was 5 pg of genomic DNA per reaction. A test performance study that included five laboratories in Germany resulted in 100% sensitivity and 97.7% specificity of the assay. Genomic regions, specific to common bunt (Tilletia caries and Tilletia laevis together) are also provided.

scholarly journals Compatible and Incompatible Interactions in Wheat Involving the Bt-10 Gene for Resistance to Tilletia tritici, the Common Bunt Pathogen

2007 ◽  
Vol 97 (11) ◽  
pp. 1397-1405 ◽  
Author(s):  
Denis A. Gaudet ◽  
Zhen-Xiang Lu ◽  
Frances Leggett ◽  
Bryan Puchalski ◽  
André Laroche
Keyword(s):  
Compatible Interaction ◽  
Incompatible Interaction ◽  
Common Bunt ◽  
Tilletia Tritici ◽  
Pathogen Invasion ◽  
Reaction Zones ◽  
The Common ◽  
Wheat Lines ◽  
Compatible Interactions ◽  
Incompatible Interactions

The infection of wheat lines Neepawa (susceptible), and its sib BW553 that is nearly isogenic for the Bt-10 resistance gene by differentially virulent races T1 and T27 of common bunt (Tilletia tritici), was followed for 21 days following seeding (dfs) using fluorescence and confocal microscopy. Spore germination was nonsynchronous and all spore stages including germination were observed 5 to 21 dfs. Initial host perception of pathogen invasion, based on autofluorescence in epidermal cells adjacent to the appressoria, was similar in both compatible and incompatible interactions, and occurred as early as 5 to 6 dfs. The total number of sites on a 1-cm segment of coleoptile adjacent to the seed that exhibited autofluorescence was similar in both the compatible and incompatible interactions and rose to a maximum of 35 to 40 per 1 cm length of coleoptile following 17 dfs, although new infection events were observed as late as 21 dfs. In the compatible interaction, the autofluorescence became more diffuse 10 to 12 dfs, emanating in all directions in association with fungal spread. In the incompatible interaction, autofluorescence remained restricted to a small area surrounding the penetration site. Two different reaction zones that extended further in tissues surrounding the penetration point in the incompatible interaction compared with the compatible interaction were identified. The accumulation of callose around invading fungal hyphae was observed during both the compatible and incompatible interactions from 8 to 21 dfs. While callose accumulation was more extensive and widespread in the incompatible interaction, it was clearly present in compatible interactions, particularly in treatments involving BW553. These results were confirmed by expression of callose synthase transcripts that were more abundant in BW553 than in Neepawa and were upregulated during pathogen infection in both compatible and incompatible interactions.

scholarly journals Variability associated with the official USDA sampling plan used to inspect export wheat shipments for Tilletia controversa spores

2001 ◽  
Vol 50 (6) ◽  
pp. 755-760 ◽  
Author(s):  
T. B. Whitaker ◽  
J. Wu ◽  
G. L. Peterson ◽  
F. G. Giesbrecht ◽  
A. S. Johansson
Keyword(s):  
Sampling Plan ◽  
Tilletia Controversa

scholarly journals Effect of some induce chemical and biological agents against (Tilletia tritici (Bjerk) and T.laevis (Kühn) causal agents of wheat Common bunt disease

2016 ◽  
Vol 13 (2) ◽  
pp. 253-260
Author(s):  
Baghdad Science Journal
Keyword(s):  
Seed Treatment ◽  
Biological Agents ◽  
Aminobutyric Acid ◽  
Systemic Resistance ◽  
Induce Systemic Resistance ◽  
Common Bunt ◽  
College Of Agriculture ◽  
Effective Microorganisms ◽  
Tilletia Tritici ◽  
Foliar Treatment

This study was conducted to evaluate the efficiency of some chemicals and biological agents to induce systemic resistance (ISR) against to wheat common bunt disease caused by the two species of fungus Tilletia tritici (Bjerk.) Wint (T. caries (Dac.) Tul.) and T. laevis Kuhn (T. foetida (Wall.) Liro. Trails in the efforts to find an alternative, safe and environmentally friendly means to control the disease. Results of this study which carried out during two consecutive seasons for the years 2012 - 2013 and 2013 - 2014 at two different environmental locations. Seed treatment by (SA 100 and 200 mg/L, 500 ?–aminobutyric acid (BABA) and 1000 mg/L, Effective Microorganisms (EM1) 40 and 150 ml/kg seeds) have led to high significant reduction in the percentage of common bunt compared with the control (plants resulting from the seeds contaminated non- treatment), While foliar treatment showed some significant differences, especially in the experiment carried out at the fields of College of Agriculture - Baghdad University compared with experiments carried out in the fields of Faculty of Agricultural Sciences -University of Sulaimania, which did not showed significant differences in most treatments. The treatment with Effective microorganisms was found efficient in reducing the infection rate compared with SA and BABA.

Tilletia controversa. [Descriptions of Fungi and Bacteria].

1983 ◽  
Author(s):  
J. M. Waller
Keyword(s):  
Winter Wheat ◽  
Geographical Distribution ◽  
Early Stage ◽  
Winter Barley ◽  
Tilletia Controversa ◽  
Dwarf Bunt

Abstract A description is provided for Tilletia controversa. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Aegilops, Agropyron, Alopecurus, Arrhenatherum, Beckmannia, Bromus, Dactylis, Elymus, Festuca, Holcus, Hordeum, Koehleria, Lolium, Poa, Secale, Triticum, Trisetum. DISEASE: Causes dwarf bunt of winter wheat, and occurs sporadically on many grasses. Occasionally infects winter barley. Infected plants develop chlorotic flecks at an early stage, are markedly stunted, and produce rather fat, persistently green ears with protuberant spikes caused by the bunt balls which fill the grain. As with T. caries (CMI Descriptions No. 719) and T. foetida (CMI Descriptions No. 720) the contents of the grain are converted to a mass of teliospores which constitute the bunt ball. GEOGRAPHICAL DISTRIBUTION: Europe (except Spain and UK); N. Africa, W. Asia, N. America, Argentina and Uruguay (CMI Map 297, ed. 2, 1968). TRANSMISSION: Teliospores are released when the grain is harvested and contaminate soil and seed. Soil-borne spores are the major source of inoculum for infecting crops which occurs between December and April in NW USA (43, 1295). Teliospores in bunt balls can remain viable in the soil for several years.

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