scholarly journals Profile of Antibodies to the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Probable SARS Patients

2004 ◽  
Vol 11 (1) ◽  
pp. 227-228 ◽  
Author(s):  
Xuan Liu ◽  
Yulin Shi ◽  
Ping Li ◽  
Linhai Li ◽  
Yanping Yi ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Healthy People ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Antibody ◽  
Antibody Titers

ABSTRACT Profiles of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (SARS)-associated coronavirus in 445 probable SARS patients and 3,749 healthy people or non-SARS patients were analyzed by antigen-capturing enzyme-linked immunosorbent assay. Antinucleocapsid antibodies were elucidated in 17.5% of the probable SARS patients 1 to 7 days after the onset of symptoms and in 80% of the patients 8 to 14 days after the onset. About 90% of the probable SARS patients were positive 15 or more days after illness. Antibody titers increased up to 70 days, and high antibody titers were maintained at least for another 3 months. Of the healthy people and non-SARS patients, only seven (0.187%) were weakly positive.

scholarly journals Use of the COOH Portion of the Nucleocapsid Protein in an Antigen-Capturing Enzyme-Linked Immunosorbent Assay for Specific and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus

2005 ◽  
Vol 12 (3) ◽  
pp. 474-476 ◽  
Author(s):  
Maofeng Qiu ◽  
Jin Wang ◽  
Hongxia Wang ◽  
Zeliang Chen ◽  
Erhei Dai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Severe Acute Respiratory Syndrome ◽  
Epidemiological Study ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sars Coronavirus ◽  
Specific Test ◽  
N Protein

ABSTRACT Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

scholarly journals Naturally Occurring Anti-Escherichia coli Protein Antibodies in the Sera of Healthy Humans Cause Analytical Interference in a Recombinant Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Severe Acute Respiratory Syndrome

2006 ◽  
Vol 14 (1) ◽  
pp. 99-101 ◽  
Author(s):  
Chi Wai Yip ◽  
Chung Chau Hon ◽  
Fanya Zeng ◽  
Ken Y. C. Chow ◽  
Kwok Hung Chan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Acute Respiratory Syndrome ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
False Positives ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Humans ◽  
Analytical Interference ◽  
Human Sera ◽  
Recombinant Nucleocapsid Protein

ABSTRACT We reported the analytical interference of anti-Escherichia coli protein (EP) antibodies in human sera and residual EP in a recombinant nucleocapsid protein-based enzyme-linked immunosorbent assay as a possible source of false positives in severe acute respiratory syndrome serodiagnosis. The rate of false positives was significantly reduced by adding mouse anti-EP antiserum in the blocking step.

scholarly journals Recombinant Truncated Nucleocapsid Protein as Antigen in a Novel Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Severe Acute Respiratory Syndrome Coronavirus Infection

2007 ◽  
Vol 14 (2) ◽  
pp. 146-149 ◽  
Author(s):  
Fuxun Yu ◽  
Mai Quynh Le ◽  
Shingo Inoue ◽  
Futoshi Hasebe ◽  
Maria del Carmen Parquet ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Seroconversion Rate ◽  
Disease Onset ◽  
Immunoglobulin M ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igm Antibody ◽  
Specificity And Sensitivity

ABSTRACT We report the development of an immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for severe acute respiratory syndrome coronavirus (SARS-CoV) by using recombinant truncated SARS-CoV nucleocapsid protein as the antigen. The newly developed MAC-ELISA had a specificity and sensitivity of 100% as evaluated by using sera from healthy volunteers and patients with laboratory-confirmed SARS. Using serial serum samples collected from SARS patients, the times to seroconversion were determined by IgM antibody detection after SARS-CoV infection. The median time to seroconversion detection was 8 days (range, 5 to 17 days) after disease onset, and the seroconversion rates after the onset of illness were 33% by the first week, 97% by the second week, and 100% by the third week. Compared with the results of our previous report on the detection of IgG, the median seroconversion time by IgM detection was 3 days earlier and the seroconversion rate by the second week after the illness for IgM was significantly higher than by IgG assay. Our results indicating that the IgM response appears earlier than IgG after SARS-CoV infection in consistent with those for other pathogens. Our newly developed MAC-ELISA system offers a new alternative for the confirmation of SARS-CoV infection.

scholarly journals Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS

2005 ◽  
Vol 125 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Masayuki Saijo ◽  
Toshio Ogino ◽  
Fumihiro Taguchi ◽  
Shuetsu Fukushi ◽  
Tetsuya Mizutani ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Nucleocapsid Protein

scholarly journals Indirect Enzyme-Linked Immunosorbent Assay based on the nucleocapsid protein of SARS-like coronaviruses

2009 ◽  
Vol 24 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Jun-fa Yuan ◽  
Yan Li ◽  
Hua-jun Zhang ◽  
Peng Zhou ◽  
Zhen-hua Ke ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Enzyme Linked Immunosorbent Assay

Comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey

1978 ◽  
Vol 8 (3) ◽  
pp. 268-276
Author(s):  
L H Ghose ◽  
R D Schnagl ◽  
I H Holmes
Keyword(s):  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Age Groups ◽  
Enzyme Reaction ◽  
Epidemiological Survey ◽  
Enzyme Linked Immunosorbent Assay ◽  
Marker Enzyme ◽  
Aminosalicylic Acid ◽  
Antibody Titers ◽  
Antibody Levels

The development of a micro-scale enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase as the marker enzyme for the detection and measurement of human rotavirus antibodies is described. A semipurified preparation of the serologically related simian agent, SA-11 virus, was used as the antigen. Test sera were reacted with antigen-sensitized wells in disposable poly-vinyl microplates. Any attached antibody was detected by the addition of peroxidase-labeled anti-species immunoglobulin (conjugate) followed by assay of the enzyme reaction with its substrate, hydrogen peroxide plus 5-aminosalicylic acid. This micro-ELISA was compared with complement fixation in a seroepidemiological study of the age prevalence of rotavirus antibody in Aboriginal and European populations living in the same outback area in Australia. The ELISA (results read with the naked eye) proved to be approximately 16 times more sensitive than complement fixation. Of Aborigines, 71% had rotavirus complement-fixing antibody, as compared to 45% of Europeans. By ELISA 100% of both populations had rotavirus antibodies. Mean antibody titers in the different age groups were higher in Aborigines than in Europeans. Antibody levels rose steeply throughout the first 20 years of life, remained high during the next 20 years, then increased again at least up to the age of 60 years. The micro-ELISA was practical, simple to perform, and more suitable than complement fixation for large seroepidemiological rotavirus studies. It also has potential for serodiagnosis of the disease, both in the laboratory and in the field.

Effect of Early Immunization on Antibody Response to Reimmunization with Measles Vaccine as Demonstrated by Enzyme-Linked Immunosorbent Assay (ELISA)

PEDIATRICS ◽  
1984 ◽  
Vol 74 (1) ◽  
pp. 90-93
Author(s):  
M. Dianne Murphy ◽  
Philip A. Brunell ◽  
Alan W. Lievens ◽  
Ziad M. Shehab
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
San Antonio ◽  
Significant Risk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Vaccine ◽  
Young Infants ◽  
Antibody Titers

A measles epidemic in San Antonio, Texas provided a population of children who were immunized at ≤10 months of age and reimmunized at ≥15 months of age. Of these children, 302 were evaluated for measles antibody by the sensitive enzyme-linked immunosorbent assay (ELISA), and their responses were compared with those of 300 children who had been immunized at the customary time (≥15 months) with a single immunization. There were only five seronegative findings in each group. The children immunized at the customary time did have significantly higher (P < .001) antibody titers than the children immunized at ≤10 months and reimmunized at ≥15 months. These results indicate that early immunization followed by reimmunization may be indicated when young infants are at significant risk of measles exposure. This approach should not create an increased number of serologically nonresponsive children when reimmunized at ≥15 months.

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