scholarly journals Persistence of Antibodies to West Nile Virus in Naturally Infected Rock Pigeons (Columba livia)

2005 ◽  
Vol 12 (5) ◽  
pp. 665-667 ◽  
Author(s):  
Samantha E. J. Gibbs ◽  
Douglas M. Hoffman ◽  
Lillian M. Stark ◽  
Nicole L. Marlenee ◽  
Bradley J. Blitvich ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Columba Livia ◽  
Maternal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Entire Study ◽  
Plaque Reduction Neutralization Test ◽  
Antibody Persistence

ABSTRACT Wild caught rock pigeons (Columba livia) with antibodies to West Nile virus were monitored for 15 months to determine antibody persistence and compare results of three serologic techniques. Antibodies persisted for the entire study as detected by epitope-blocking enzyme-linked immunosorbent assay and plaque reduction neutralization test. Maternal antibodies in squabs derived from seropositive birds persisted for an average of 27 days.

scholarly journals Assessment of Mumps Virus-Specific Antibodies: Comparison of Plaque Reduction Neutralization Test and Enzyme-Linked Immunosorbent Assay Estimates

2019 ◽  
Vol 220 (9) ◽  
pp. 1462-1468 ◽  
Author(s):  
Stéphanie Ravault ◽  
Damien Friel ◽  
Emmanuel Di Paolo ◽  
Adrian Caplanusi ◽  
Paul Gillard ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Pearson Correlation ◽  
Mumps Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Moderate Correlation ◽  
Plaque Reduction Neutralization Test

Abstract Background The plaque reduction neutralization test (PRNT), which measures a subset of immunoglobulin antibodies (functional neutralizing antibodies), and the enzyme-linked immunosorbent assay (ELISA), which measures total immunoglobulin (neutralizing and nonneutralizing antibodies), characterize different aspects of the anti–mumps virus antibody response after vaccination. Methods Data from a recent phase 3 clinical trial (NCT01681992) of 2 measles-mumps-rubella vaccines were used to compare anti-mumps antibody responses measured using an unenhanced PRNT (GSK; seropositivity cutoff and threshold, 2.5 and 4 times the 50% end-point dilution, respectively) with those estimated using an ELISA (thresholds, 5 and 10 ELISA units/mL, respectively). Results Of 3990 initially seronegative samples, 3284 (82.3%) were seropositive after vaccination for anti-mumps antibodies in both assays. The Pearson correlation coefficient for double-positive samples was 0.57, indicative of a moderate correlation. Receiver operating characteristic curve analysis showed that an ELISA threshold of 51.7 ELISA units/mL best corresponded to the PRNT seroresponse threshold. There was no obvious vaccine brand effect on the correlation between assays. Conclusions The moderate correlation between the anti-mumps antibody measurements obtained with PRNT and ELISA reflects different aspects of the serological response. In the absence of a well-defined protective serological threshold, PRNT provides complementary information on the antibody response, whereas ELISA remains a critically useful measurement of vaccine immunogenicity.

scholarly journals Serum Neutralization Assay Can Efficiently Replace Plaque Reduction Neutralization Test for Detection and Quantitation of West Nile Virus Antibodies in Human and Animal Serum Samples

2014 ◽  
Vol 21 (10) ◽  
pp. 1460-1462 ◽  
Author(s):  
Annapia Di Gennaro ◽  
Alessio Lorusso ◽  
Claudia Casaccia ◽  
Annamaria Conte ◽  
Federica Monaco ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Neutralization Assay ◽  
Serum Samples ◽  
West Nile ◽  
Plaque Reduction Neutralization Test ◽  
Serum Neutralization ◽  
Human Sera

ABSTRACTA serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.

scholarly journals Evaluation of Euroimmun Anti-Zika Virus IgM and IgG Enzyme-Linked Immunosorbent Assays for Zika Virus Serologic Testing

2017 ◽  
Vol 55 (8) ◽  
pp. 2462-2471 ◽  
Author(s):  
Arnaud G. L'Huillier ◽  
Anne Hamid-Allie ◽  
Erik Kristjanson ◽  
Louis Papageorgiou ◽  
Sam Hung ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Zika Virus ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Convenience Sample ◽  
Plaque Reduction Neutralization Test ◽  
Serologic Diagnosis ◽  
Serologic Testing ◽  
Antibody Capture

ABSTRACTWith the emerging Zika virus (ZIKV) epidemic, serologic diagnosis relies on a labor-intensive IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and confirmation by a plaque reduction neutralization test (PRNT). To streamline serologic testing, several commercial assays have been developed. Our aim was to compare the commercial Euroimmun anti-ZIKV IgM and IgG assays to the reference MAC-ELISA and PRNT currently in use. Serum specimens submitted to Public Health Ontario Laboratory, Canada, were tested for IgM and IgG using the Euroimmun assays and the results were compared with those from MAC-ELISA. The PRNT was performed on positive or equivocal specimens using either MAC-ELISA or Euroimmun assays, MAC-ELISA-inconclusive specimens, and a convenience sample of specimens negative by both assays (cohort 1). Another set of specimens selected on the basis of PRNT results was subsequently tested by the Euroimmun assays (cohort 2). MAC-ELISA was positive, equivocal, negative, and inconclusive in 57/223, 15/223, 147/223, and 4/223 specimens, respectively. Among the 76 specimens that were MAC-ELISA positive, equivocal, or inconclusive, 30 (39.5%) were Euroimmun IgM and/or IgG positive or equivocal. Among the 147 MAC-ELISA-negative specimens, 136 (92.5%) were Euroimmun IgM and IgG negative. The sensitivity of the combined Euroimmun IgM/IgG against the PRNT was 83% (cohort 1) and 92% (cohort 2), whereas the specificity was 81% (cohort 1) and 65% (cohort 2). The combined Euroimmun IgM/IgG showed good specificity (92.5%) but suboptimal sensitivity (39.5%) compared with that of the MAC-ELISA. However, the sensitivity of the combined Euroimmun IgM/IgG against the PRNT was significantly higher (83 to 92%). More studies are needed before commercial assays are implemented for routine ZIKV serologic diagnosis.

Determination of human IgG and IgM class antibodies to West Nile virus by enzyme linked immunosorbent assay (ELISA)

1985 ◽  
Vol 17 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Sabine Feinstein ◽  
Yair Akov ◽  
Bat-El Lachmi ◽  
Shoshana Lehrer ◽  
Lotte Rannon ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Human Igg

The continuous spread of West Nile virus (WNV): seroprevalence in asymptomatic horses

2009 ◽  
Vol 137 (8) ◽  
pp. 1163-1168 ◽  
Author(s):  
J. ALONSO-PADILLA ◽  
E. LOZA-RUBIO ◽  
E. ESCRIBANO-ROMERO ◽  
L. CÓRDOBA ◽  
S. CUEVAS ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Gulf Of Mexico ◽  
Neutralization Test ◽  
Haemagglutination Inhibition ◽  
Igg Antibodies ◽  
West Nile ◽  
Plaque Reduction Neutralization Test ◽  
Northern Mexico ◽  
Independent Events ◽  
The Usa

SUMMARYWest Nile virus (WNV) was probably introduced in southern and northern Mexico from the USA in two independent events. Since then, WNV activity has been reported in several Mexican states bordering the USA and the Gulf of Mexico, but disease manifestations seen there in humans and equids are quite different to those observed in the USA. We have analysed WNV seroprevalence in asymptomatic, unvaccinated equids from two Mexican states where no data had been previously recorded. WNV IgG antibodies were detected in 31·6% (91/288) of equine sera from Chiapas and Puebla states (53·3% and 8·0%, respectively). Analysis by plaque reduction neutralization test (PRNT) showed good specificity (99·4%) and sensitivity (84·9%) with the ELISA results. Further analyses to detect antibodies against three different flaviviruses (WNV, St Louis encephalitis virus, Ilheus virus) by haemagglutination inhibition (HI) tests on a subset of 138 samples showed that 53% of the 83 HI-positive samples showed specific reaction to WNV. These data suggest continuous expansion of WNV through Mexico.

A Recombinant Envelope Protein-Based Enzyme-Linked Immunosorbent Assay for West Nile Virus Serodiagnosis

2002 ◽  
Vol 2 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Tian Wang ◽  
Louis A. Magnarelli ◽  
John F. Anderson ◽  
L. Hannah Gould ◽  
Sandra L. Bushmich ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Immunosorbent Assay ◽  
Envelope Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
West Nile ◽  
Recombinant Envelope Protein
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