scholarly journals Development of Novel Immunoglobulin G (IgG), IgA, and IgM Enzyme Immunoassays Based on Recombinant Puumala and Dobrava Hantavirus Nucleocapsid Proteins

2006 ◽  
Vol 13 (12) ◽  
pp. 1349-1357 ◽  
Author(s):  
Helga Meisel ◽  
Anne Wolbert ◽  
Ausra Razanskiene ◽  
Andreas Marg ◽  
Andris Kazaks ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Hemorrhagic Fever ◽  
Virus Infections ◽  
Serum Samples ◽  
Nucleocapsid Proteins ◽  
Dobrava Virus ◽  
Acute Infections ◽  
Antibody Capture ◽  
Human Infections ◽  
Heterologous Antigens

ABSTRACT Human infections with Asian and European hantaviruses can result in hemorrhagic fever with renal syndromes of differing severities characterized by renal dysfunction and sometimes by pulmonary symptoms. For the serological detection of human infections by hantaviruses relevant for Europe, we developed monoclonal antibody capture immunoglobulin G (IgG) and IgA enzyme-linked immunosorbent assays (ELISAs) based on yeast-expressed nucleocapsid proteins of Puumala and Dobrava hantaviruses. Moreover, for diagnosis of acute infections, μ-capture IgM ELISAs were established with nucleocapsid proteins expressed in Drosophila melanogaster Schneider S2 cells. The cutoff values of the ELISAs were determined by investigation of up to 500 human anti-hantavirus-negative serum samples. The specificities of the Puumala and Dobrava virus-specific IgM, IgA, and IgG ELISAs were found to be 100%. The sensitivities of these ELISAs were determined to be 100% with panels of characterized anti-Puumala or anti-Dobrava virus-positive human serum samples. In most cases, Puumala and Dobrava virus infections could be differentiated by ELISA reactivity alone, i.e., endpoint titration with homologous and heterologous antigens.

scholarly journals A Patient with Crimean-Congo Hemorrhagic Fever Serologically Diagnosed by Recombinant Nucleoprotein-Based Antibody Detection Systems

2003 ◽  
Vol 10 (3) ◽  
pp. 489-491 ◽  
Author(s):  
Qing Tang ◽  
Masayuki Saijo ◽  
Yuzhen Zhang ◽  
Muer Asiguma ◽  
Dong Tianshu ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Hemorrhagic Fever ◽  
Antibody Detection ◽  
Virus Infections ◽  
Virus Antibody ◽  
Serum Samples ◽  
Crimean Congo Hemorrhagic Fever ◽  
Detection Systems ◽  
Reverse Transcription Pcr ◽  
Cchf Virus

ABSTRACT We treated a male patient with Crimean-Congo hemorrhagic fever (CCHF). The diagnosis of CCHF was confirmed by reverse transcription-PCR and recombinant nucleoprotein (rNP)-based immunoglobulin G (IgG) and IgM capture enzyme-linked immunosorbent assays of serially collected serum samples. The patient was treated with intravenous ribavirin and recovered with no consequences. The study indicates that rNP-based CCHF virus antibody detection systems are useful for confirming CCHF virus infections. This case also suggests that intravenous ribavirin therapy may be promising for the treatment of CCHF patients.

scholarly journals Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Serodiagnosis of Dengue Virus Infection

2005 ◽  
Vol 12 (10) ◽  
pp. 1235-1237 ◽  
Author(s):  
M. Nawa ◽  
T. Takasaki ◽  
M. Ito ◽  
S. Inoue ◽  
K. Morita ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Iga Antibody ◽  
Antibody Capture

ABSTRACT We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.

scholarly journals Development of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros
Keyword(s):  
Immunoglobulin G ◽  
Rubella Virus ◽  
Primary Infection ◽  
Virus Infections ◽  
Serum Samples ◽  
Igg Avidity ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Semiautomated Method

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.

scholarly journals Serotype-Cross-Reactive Immunoglobulin M Responses in Dengue Virus Infections Determined by Enzyme-Linked Immunosorbent Assay

2000 ◽  
Vol 7 (5) ◽  
pp. 774-777 ◽  
Author(s):  
Masaru Nawa ◽  
Ken-Ichiro Yamada ◽  
Tomohiko Takasaki ◽  
Toshitaka Akatsuka ◽  
Ichiro Kurane
Keyword(s):  
Dengue Virus ◽  
Japanese Patients ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Reverse Transcription Pcr ◽  
Virus Serotype ◽  
Dengue Virus Serotypes ◽  
Antibody Capture

ABSTRACT We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.

scholarly journals COMPARISON WITH INTENSITY OF SECONDARY DENGUE INFECTION BY DETECTING DENGUE-SPECIFIC IMMUNOGLOBULIN G ANTIBODIES

2018 ◽  
Vol 11 (12) ◽  
pp. 120
Author(s):  
Sudipta Poddar ◽  
Amiya Kumar Hati
Keyword(s):  
Immunoglobulin G ◽  
West Bengal ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Objective Detection ◽  
The City

Objective: Detection of dengue-specific immunoglobulin G (IgG) antibodies in the serum of healthy individuals signifies previous dengue infection. This phenomenon can be utilized to demonstrate dengue activity in a suspected area and to stratify intensity of infection in the urban and rural surroundings.Methods: Serum samples of altogether 881 healthy volunteers, 560 persons living in the central part of the city Kolkata and 321 individuals residing at a village Memari (highly endemic for dengue), 85 Km away from Kolkata, having no apparent proof of dengue activity were examined for the detection of dengue-specific IgG antibodies.Results: Of 560 serum samples collected from Kolkata, 55.5% (249) were IgG reactive. Respective figures for Memari were 321 and 25.3% (81). This indicated that the virus was also active at rural area, though the endemic property was lower than in Kolkata. In both urban (r=−0.585, p=0.017) and rural (r=−0.392, p=0.013) West Bengal, the suspected and IgG reactive cases apparently negatively correlated with age, but the percentage of reactivity was found to increase with age. The number of IgG reactive cases was significantly more in Kolkata than at Memari, indicating that dengue virus was active at Memari though the endemic property was much lower than that of Kolkata and chances of secondary dengue infection vis-a-vis dengue hemorrhagic fever would be more in Kolkata than at Memari.Conclusion: Evaluation of IgG specific dengue antibodies can be utilized to compare intensity of secondary dengue infection vis-a-vis DHF and its endemic property in different places, furnish a preliminary idea.

scholarly journals Use of Immunoglobulin M Cross-Reactions in Differential Diagnosis of Human Flaviviral Encephalitis Infections in the United States

2002 ◽  
Vol 9 (3) ◽  
pp. 544-549 ◽  
Author(s):  
Denise A. Martin ◽  
Brad J. Biggerstaff ◽  
Becky Allen ◽  
Alison J. Johnson ◽  
Robert S. Lanciotti ◽  
...  
Keyword(s):  
The United States ◽  
Virus Antigen ◽  
Cross Reactivity ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Heterologous Virus ◽  
Antibody Capture ◽  
Virus Antigens

ABSTRACT To define the virus specificity of the immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) among the medically important members of the Japanese encephalitis (JE) virus serocomplex of flaviviruses, 103 IgM-positive human serum samples from patients with confirmed West Nile (WN) virus, St. Louis encephalitis (SLE) virus, or JE virus infections were assembled and simultaneously tested against all three viral antigens in a standardized MAC-ELISA. Of the serum samples tested, 96 (93%) showed higher positive-to-negative absorbance ratios (P/Ns) with the infecting virus antigen compared to those obtained with the other two virus antigens. Of the seven specimens with higher P/Ns with heterologous virus antigens, six were from patients with SLE virus infections (the serum samples had higher levels of reactivity with WN virus antigen) and one was from a patient with a JE virus infection (this serum sample also had a higher level of reactivity with WN virus antigen). Not surprisingly, similar virus specificity was observed with WN virus-elicited IgM in cerebrospinal fluid. As shown in previous studies, a subset of these specimens was even less reactive in the MAC-ELISA with dengue virus, a member of a different flavivirus serocomplex. The degree of virus cross-reactivity did not appear to be related to days postonset, at least during the first 40 days of infection. Infections with WN virus could be correctly distinguished from infections with SLE virus on the basis of the observed anti-viral IgM cross-reactivities alone 92% of the time. Infections with SLE virus resulted in antibody that was more cross-reactive, so identification of SLE virus as the infecting agent by use of MAC-ELISA cross-reactivity alone was more problematic.

scholarly journals Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies

2019 ◽  
Vol 65 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Anne Rackow ◽  
Christa Ehmen ◽  
Ronald von Possel ◽  
Raquel Medialdea-Carrera ◽  
David Brown ◽  
...  
Keyword(s):  
Zika Virus ◽  
Specific Binding ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Size Exclusion ◽  
Animal Origin ◽  
Serum Samples ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Igm Antibodies

Abstract BACKGROUND The cellular surface molecule HsTOSO/FAIM3/HsFcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain (HsFcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV). METHODS His-tagged HsFcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of HsFcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on HsFcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate. RESULTS Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with HsFcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of HsFcμR-Igl as a capture molecule in aggregation-based rapid tests. CONCLUSIONS Recombinant HsFcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests.

scholarly journals Dobrava Virus Carried by the Yellow-Necked Field Mouse Apodemus flavicollis, Causing Hemorrhagic Fever with Renal Syndrome in Romania

2014 ◽  
Vol 14 (5) ◽  
pp. 358-364 ◽  
Author(s):  
Raluca Ioana Panculescu-Gatej ◽  
Anca Sirbu ◽  
Sorin Dinu ◽  
Maria Waldstrom ◽  
Paul Heyman ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Field Mouse ◽  
Apodemus Flavicollis ◽  
Dobrava Virus

scholarly journals Pathogen Dose in Animal Models of Hemorrhagic Fever Virus Infections and the Potential Impact on Studies of the Immune Response

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 275
Author(s):  
Bryce M. Warner
Keyword(s):  
Immune Response ◽  
Animal Models ◽  
Hemorrhagic Fever ◽  
Virus Infections ◽  
Critical Determinant ◽  
Animal Models Of Disease ◽  
Hemorrhagic Fever Virus ◽  
Immune Correlates ◽  
Clinical Drug Development ◽  
Wide Range

Viral hemorrhagic fever viruses come from a wide range of virus families and are a significant cause of morbidity and mortality worldwide each year. Animal models of infection with a number of these viruses have contributed to our knowledge of their pathogenesis and have been crucial for the development of therapeutics and vaccines that have been approved for human use. Most of these models use artificially high doses of virus, ensuring lethality in pre-clinical drug development studies. However, this can have a significant effect on the immune response generated. Here I discuss how the dose of antigen or pathogen is a critical determinant of immune responses and suggest that the current study of viruses in animal models should take this into account when developing and studying animal models of disease. This can have implications for determination of immune correlates of protection against disease as well as informing relevant vaccination and therapeutic strategies.

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