scholarly journals Dense-Granule Protein NcGRA7, a New Marker for the Serodiagnosis of Neospora caninum Infection in Aborting Cows

2007 ◽  
Vol 14 (12) ◽  
pp. 1640-1643 ◽  
Author(s):  
Penglong Huang ◽  
Min Liao ◽  
Houshuang Zhang ◽  
Eung-goo Lee ◽  
Yoshifumi Nishikawa ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Neospora Caninum ◽  
Dense Granule ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Caninum Infection ◽  
Recombinant Antigens ◽  
Granule Protein ◽  
Bovine Abortion

ABSTRACT To investigate whether the production of an antigen-specific antibody is associated with Neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent assay using the recombinant antigens NcSAG1, NcSRS2, and NcGRA7. Our study suggested that NcGRA7 would be a new marker for the serodiagnosis of N. caninum infection resulting in abortion.

scholarly journals Evidence of Neospora caninum exposure among native Korean goats (Capra hircus coreanae)

2014 ◽  
Vol 59 (No. 12) ◽  
pp. 637-640 ◽  
Author(s):  
BY Jung ◽  
SH Lee ◽  
D. Kwak
Keyword(s):  
Neospora Caninum ◽  
Control Strategies ◽  
Integrated Control ◽  
Protozoan Parasite ◽  
Capra Hircus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Caninum Infection ◽  
One Year ◽  
And Control

Neospora caninum is a protozoan parasite that causes abortion in ruminants, including goats. The objective of the present study was to determine the seroprevalence of N. caninum in native Korean goats (Capra hircus coreanae). A commercial enzyme-linked immunosorbent assay kit was used to analyse 464 serum samples for the presence of N. caninum antibodies. Four samples (0.9%, 95% confidence intervals – CI: 0.0–1.7) were found to be positive for N. caninum antibodies. The seroprevalence was analysed according to age (less than to one year, young; more than or equal one year, adult; and unknown), sampling season (April to September, warm; October to March, cold), and region (northern, central, and southern). However, there were no statistically significant differences in seroprevalence according to age, season, and region (P > 0.05). This is the first report on the seroprevalence of N. caninum in native Korean goats. The results of this study indicate a nationwide distribution of N. caninum among goats, with a relatively low prevalence. Therefore, the implementation of integrated control strategies as well as measures for prevention and control of N. caninum infection among goats is recommended.

scholarly journals Development of Rapid Immunochromatographic Test with Recombinant NcSAG1 for Detection of Antibodies to Neospora caninum in Cattle

2005 ◽  
Vol 12 (7) ◽  
pp. 885-887 ◽  
Author(s):  
Min Liao ◽  
Shoufa Zhang ◽  
Xuenan Xuan ◽  
Guohong Zhang ◽  
Xiaohong Huang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Reliable Method ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Serum Samples ◽  
Immunofluorescent Antibody Test ◽  
Good Agreement

ABSTRACT An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.

scholarly journals Seroprevalence and risk factors of Neospora caninum infection among domestic sheep in Henan province, central China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 15 ◽  
Author(s):  
Shuai Wang ◽  
Lingjuan Li ◽  
Yi Lu ◽  
Haizhu Zhang ◽  
Qing Xie ◽  
...  
Keyword(s):  
Risk Factors ◽  
Neospora Caninum ◽  
Henan Province ◽  
Central China ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Sheep ◽  
Serum Samples ◽  
Caninum Infection ◽  
Positive Rate ◽  
Associated Risk Factors

This study aimed to determine the frequency of antibodies to Neospora caninum in domestic sheep raised in Henan province, central China. Serum samples from 779 domestic sheep were collected from March 2015 to May 2016, and antibodies to N. caninum were evaluated using an enzyme-linked immunosorbent assay (ELISA). The results showed an overall IgG positive rate of 7.32% (57/779). The risk factors significantly related to seropositivity to N. caninum in sheep were the age, the presence of dogs, and the rearing system. This is the first report of N. caninum infection and associated risk factors in domestic sheep in central China.

scholarly journals Serological response to Neospora caninum infection in goats and agreement between three diagnostic techniques to detect caprine neosporosis

2019 ◽  
Vol 39 (1) ◽  
pp. 25-31
Author(s):  
Pomy C.P. Kim ◽  
Renata P.B. Melo ◽  
Jonatas C. Almeida ◽  
José G. Silva ◽  
Muller Ribeiro-Andrade ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
High Sensitivity ◽  
Antibody Test ◽  
Diagnostic Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Serum Samples ◽  
Caninum Infection ◽  
Reference Serum ◽  
Indirect Immunofluorescent

ABSTRACT: The present study aimed to measure the serological response of goats infected with Neospora caninum by assessing the diagnostic performance and agreement between three techniques (indirect immunofluorescent antibody test, IFAT; Neospora agglutitation test, NAT; enzyme-linked immunosorbent assay, ELISA). The panel of sera were comprised of 500 samples of goats, and 60 reference serum samples. These reference and field serum samples were tested by ELISA, NAT, and IFAT. In the field serum samples tested, the seroprevalences of anti-N. caninum antibodies were 3.2%, 4.6%, and 6.4% in the NAT, IFAT and ELISA, respectively. Using the IFAT as the gold standard, the NAT and the ELISA agreement was considered weak (k=0.28) and strong (k=0.75), respectively. When the IFAT performance was used for comparison purposes, the ELISA showed 91.3% sensitivity and 97.7%, specificity with a PPV of 65.2% and a NPV of 99.6%; The NAT presented sensitivity of 26.1% and specificity of 97.9% with a PPV of 37.5% and a NPV of 96.5%. Accordingly, the IFAT should remain the assay of choice for studies about N. caninum infection in goats in individual serum samples. A combination of serological assays with high sensitivity and specificity is recommended in serosurveys of caprine neosporosis.

scholarly journals Development of an Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of Toxoplasma gondii Infection

2013 ◽  
Vol 20 (4) ◽  
pp. 596-601 ◽  
Author(s):  
Mohamad Alaa Terkawi ◽  
Kyohko Kameyama ◽  
Nazim Hamza Rasul ◽  
Xuean Xuan ◽  
Yoshifumi Nishikawa
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Latex Agglutination ◽  
Dense Granule ◽  
Diagnostic Tools ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Content Type ◽  
Specificity And Sensitivity ◽  
Field Samples

ABSTRACTDense granule antigen proteins derived fromToxoplasma gondii(TgGRAs) are potential antigens for the development of diagnostic tools. TgGRA7 and TgGRA14 were detected in the peritoneal fluid ofT. gondii-infected mice, suggesting that TgGRAs may be highly antigenic proteins. Here, TgGRA7 and TgGRA14 were evaluated as candidates for the development of a marker for a rapid diagnostic test. The specificity and sensitivity of purified recombinant proteins of TgGRA7 and TgGRA14 were compared in an indirect enzyme-linked immunosorbent assay (iELISA) using a series of serum samples fromT. gondii-experimentally infected mice and using recombinantT. gondiimajor surface antigen 2 (TgSAG2) as a reference control. The iELISA with TgGRA7 showed the greatest diagnostic accuracy and could detect anti-TgGRA7 antibody in acute and chronic infections. A total of 59 field samples from pigs were also examined by the iELISAs, and the results compared with those of the latex agglutination test (LAT). Among the three recombinant antigens, TgGRA7 had the highest rates of positivity, with significant concordance (88.14) and kappa value (0.76) in comparison with the results using LAT. Furthermore, an immunochromatographic test (ICT) based on recombinant TgGRA7 was developed for rapid detection of antibodies to the infection. The ICT differentiated clearly between sera fromT. gondii-infected mice and uninfected orNeospora caninum-infected mice. Pig sera were examined with the ICT, and the results compared favorably with those of LAT and iELISA for TgGRA7, with kappa values of 0.66 and 0.70 to 0.79, respectively. These data suggest that the ICT based on TgGRA7 is a promising diagnostic tool for routine testing in the clinic and mass screening of samples in the field.

scholarly journals  Toxoplasma gondii and Neospora caninum antibodies in goats in the Czech Republic

2012 ◽  
Vol 57 (No. 3) ◽  
pp. 111-114 ◽  
Author(s):  
E. Bartova ◽  
K. Sedlak
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Antibodies ◽  
Serum Samples ◽  
The Czech Republic

Toxoplasma gondii is zoonotic protozoan parasite that causes infections in many vertebrate species. The present study determined the seroprevalence of T. gondii and N. caninum in goats from the Czech Republic. Serum samples were collected from 251 healthy adult goats in the Czech Republic during the years 2006 to 2009. Sera samples were tested for serum antibodies to Toxoplasma gondii by an enzyme-linked immunosorbent assay with cut off equal to or higher than 50% S/P. The same samples were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay with cut off equal to or higher than 30% inhibition; positive sera were confirmed by an indirect fluorescent antibody test with cut-off titre equal to or higher than 40. Sera positive in both tests were marked as positive. In total, 166 (66%) and 15 (6%) goat sera reacted positively for T. gondii and N. caninum antibodies, respectively. All sera positive for N. caninum antibodies were simultaneously positive for T. gondii antibodies. This is the first detection of N. caninum antibodies in goats in the Czech Republic. Our findings indicate that goats in the Czech Republic are frequently exposed to T. gondii, but less frequently to N. caninum.  

scholarly journals Use of Avidity Enzyme-Linked Immunosorbent Assay and Avidity Western Blot to Discriminate between Acute and Chronic Neospora Caninum Infection in Cattle

2005 ◽  
Vol 17 (5) ◽  
pp. 442-450 ◽  
Author(s):  
A. Aguado-Martínez ◽  
G. Álvarez-García ◽  
I. Arnaiz-Seco ◽  
E. Innes ◽  
L. M. Ortega-Mora
Keyword(s):  
Western Blot ◽  
Chronic Infection ◽  
Primary Infection ◽  
Neospora Caninum ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Avidity ◽  
Infected Cattle ◽  
Serum Samples ◽  
Caninum Infection ◽  
Igg Avidity

Avidity serological tests (avidity enzyme-linked immunosorbent assay [ELISA] and avidity Western blot) were developed and used to differentiate between acute (primary infection, reinfection, and recrudescence) and chronic Neospora caninum infection in cattle. In addition, the pattern of immunoglobulin G (IgG) avidity maturation against different specific antigens of N. caninum tachyzoites was studied. Sequential serum samples were collected from cattle naturally and experimentally infected with N. caninum. Four groups of experimentally infected cattle were included in the study and were representative of primary infection, reinfection, chronic infection, and noninfection. Serum samples were also collected from naturally infected cattle classified into nonaborting and aborting cows on the basis of clinical findings and serological profiles, and a third group composed of seronegative cows that seroconverted during the course of the experiment. All samples were tested by avidity ELISA and avidity Western blot. The IgG avidity ELISA allowed the discrimination between primary and chronic infection because all experimentally primary-infection cows showed low avidity indexes at week 4 postinfection (p.i.) compared with the high avidity values found at week 20 postinfection. However, this test did not allow the discrimination of reinfection or recrudescence from chronic infection. Regarding IgG avidity Western blot results, no antigenic markers correlating with acute (primary infection, recrudescence, and reinfection) or chronic infection were recognized. However, the 17-kD immunodominant antigen was mostly responsible for high avidity values obtained by avidity ELISA because it was intensively recognized by high-avidity antibodies in all chronically infected animals after urea treatment.

Sign in / Sign up

Export Citation Format

Share Document