scholarly journals Pulmonary Mycobacterium bovis BCG Vaccination Confers Dose-Dependent Superior Protection Compared to That of Subcutaneous Vaccination

2014 ◽  
Vol 21 (4) ◽  
pp. 594-597 ◽  
Author(s):  
Nacho Aguilo ◽  
Ana Maria Toledo ◽  
Eva Maria Lopez-Roman ◽  
Esther Perez-Herran ◽  
Eamonn Gormley ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Bovis ◽  
Dose Range ◽  
Bcg Vaccine ◽  
Content Type ◽  
Mycobacterium Bovis Bcg ◽  
Bcg Vaccination ◽  
Dose Dependent

ABSTRACTWorldwide, theMycobacterium bovisBCG vaccine is one of the most widely used vaccines. However, it appears to be ineffective in preventing pulmonary tuberculosis. Here, we show that pulmonary BCG vaccination of mice with a broad dose range provides superior protection againstMycobacterium tuberculosischallenge compared to that of subcutaneous vaccination.

scholarly journals Complete Genome Sequence of Mycobacterium bovis BCG SL222 Sofia, the First WHO Reference Reagent for the M. bovis BCG Vaccine of the Russian BCG-I Substrain

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Stefan Panaiotov ◽  
Yordan Hodzhev ◽  
Vladimir Tolchkov ◽  
Alexander Mihailov ◽  
Roumen Kofinov ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Vaccine Strain ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Bcg Vaccine ◽  
Vaccine Production ◽  
Content Type ◽  
Mycobacterium Bovis Bcg

ABSTRACT Mycobacterium bovis BCG SL222 Sofia is a substrain descending from the Russian BCG-I vaccine strain. Here, we report the complete genome sequence of BCG SL222 Sofia, which will facilitate identity assurance and will contribute to more consistent manufacturing, standardization, and differentiation of substrains used in vaccine production.

Mycobacterium bovis BCG vaccination modulates TNF-α production after pulmonary challenge with virulent Mycobacterium tuberculosis in guinea pigs

Tuberculosis ◽  
2007 ◽  
Vol 87 (2) ◽  
pp. 155-165 ◽  
Author(s):  
Toshiko Yamamoto ◽  
Todd M. Lasco ◽  
Kazuyuki Uchida ◽  
Yoshitaka Goto ◽  
Amminikutty Jeevan ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Guinea Pigs ◽  
Mycobacterium Bovis Bcg ◽  
Bcg Vaccination ◽  
Tnf Α

scholarly journals The Protective Effect of the Mycobacterium bovis BCG Vaccine Is Increased by Coadministration with the Mycobacterium tuberculosis 72-Kilodalton Fusion Polyprotein Mtb72F in M. tuberculosis-Infected Guinea Pigs

2004 ◽  
Vol 72 (11) ◽  
pp. 6622-6632 ◽  
Author(s):  
Lise Brandt ◽  
Yasir A. W. Skeiky ◽  
Mark R. Alderson ◽  
Yves Lobet ◽  
Wilfried Dalemans ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Guinea Pig ◽  
Mycobacterium Bovis ◽  
Guinea Pigs ◽  
Airway Remodeling ◽  
Bacterial Load ◽  
Bcg Vaccine ◽  
Protective Capacity ◽  
Bcg Vaccination ◽  
Lung Consolidation

ABSTRACT A tuberculosis vaccine candidate consisting of a 72-kDa polyprotein or fusion protein based upon the Mtb32 and Mtb39 antigens of Mycobacterium tuberculosis and designated Mtb72F was tested for its protective capacity as a potential adjunct to the Mycobacterium bovis BCG vaccine in the mouse and guinea pig models of this disease. Formulation of recombinant Mtb72F (rMtb72F) in an AS02A adjuvant enhanced the Th1 response to BCG in mice but did not further reduce the bacterial load in the lungs after aerosol challenge infection. In the more stringent guinea pig disease model, rMtb72F delivered by coadministration with BCG vaccination significantly improved the survival of these animals compared to BCG alone, with some animals still alive and healthy in their appearance at >100 weeks post-aerosol challenge. A similar trend was observed with guinea pigs in which BCG vaccination was boosted by DNA vaccination, although this increase was not statistically significant due to excellent protection conferred by BCG alone. Histological examination of the lungs of test animals indicated that while BCG controls eventually died from overwhelming lung consolidation, the majority of guinea pigs receiving BCG mixed with rMtb72F or boosted twice with Mtb72F DNA had mostly clear lungs with minimal granulomatous lesions. Lesions were still prominent in guinea pigs receiving BCG and the Mtb72F DNA boost, but there was considerable evidence of lesion healing and airway remodeling and reestablishment. These data support the hypothesis that the coadministration or boosting of BCG vaccination with Mtb72F may limit the lung consolidation seen with BCG alone and may promote lesion resolution and healing. Collectively, these data suggest that enhancing BCG is a valid vaccination strategy for tuberculosis that is worthy of clinical evaluation.

scholarly journals Dose-Dependent Immune Response to Mycobacterium bovis BCG Vaccination in Neonates

2006 ◽  
Vol 14 (2) ◽  
pp. 198-200 ◽  
Author(s):  
Virginia Davids ◽  
Willem Hanekom ◽  
Sebastian J. Gelderbloem ◽  
Anthony Hawkridge ◽  
Gregory Hussey ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
T Cell ◽  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Cell Frequency ◽  
Mycobacterium Bovis Bcg ◽  
Bcg Vaccination ◽  
Cytokine Levels ◽  
Dose Dependent

ABSTRACT In 10-week-old infants vaccinated at birth with Japanese Mycobacterium bovis BCG, the number of dermal needle penetrations correlated positively with frequency of proliferating CD4+ T cells in whole blood following BCG stimulation for 6 days but did not correlate with secreted cytokine levels after 7 h or interferon CD4+ T-cell frequency after 12 h of BCG stimulation.

scholarly journals Differential Mycobacterium bovis BCG Vaccine-Derived Efficacy in C3Heb/FeJ and C3H/HeOuJ Mice Exposed to a Clinical Strain of Mycobacterium tuberculosis

2014 ◽  
Vol 22 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Marcela Henao-Tamayo ◽  
Andrés Obregón-Henao ◽  
Elizabeth Creissen ◽  
Crystal Shanley ◽  
Ian Orme ◽  
...  
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Vaccine Efficacy ◽  
Disease Process ◽  
Bcg Vaccine ◽  
Clinical Strain ◽  
Interleukin 17 ◽  
Content Type ◽  
The Impact

ABSTRACTThe global epidemic caused by the bacterial pathogenMycobacterium tuberculosiscontinues unabated. Moreover, the only available vaccine against tuberculosis,Mycobacterium bovisbacillus Calmette-Guérin (BCG), demonstrates variable efficacy. To respond to this global threat, new animal models that mimic the pathological disease process in humans are required for vaccine testing. One new model, susceptible C3Heb/FeJ mice, is similar to human tuberculosis in that these animals are capable of forming necrotic tubercle granulomas, in contrast to resistant C3H/HeOuJ mice. In this study, we evaluated the impact of prior BCG vaccination of C3Heb/FeJ and C3H/HeOuJ mice on exposure to a low-dose aerosol ofMycobacterium tuberculosisW-Beijing strain SA161. Both BCG-vaccinated murine strains demonstrated reduced bacterial loads 25 days after infection compared to controls, indicating vaccine efficacy. However, during chronic infection, vaccine efficacy waned in C3H/HeOuJ but not in C3Heb/FeJ mice. Protection in vaccinated C3Heb/FeJ mice was associated with reduced numbers of CD11b+Gr1+cells, increased numbers of effector and memory T cells, and an absence of necrotic granulomas. BCG vaccine efficacy waned in C3H/HeOuJ mice, as indicated by reduced expression of gamma interferon (IFN-γ) and increased expressions of interleukin-17 (IL-17), IL-10, and Foxp3 by T cells compared to C3Heb/FeJ mice. This is the first murine vaccine model system described to date that can be utilized to dissect differential vaccine-derived immune efficacy.

scholarly journals Mycobacterium bovis BCG Immunization Induces Protective Immunity against Nine Different Mycobacterium tuberculosis Strains in Mice

2008 ◽  
Vol 76 (11) ◽  
pp. 5173-5180 ◽  
Author(s):  
Bo Young Jeon ◽  
Steven C. Derrick ◽  
JaeHyun Lim ◽  
Kristopher Kolibab ◽  
Veerabadran Dheenadhayalan ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Bovis ◽  
Lung Inflammation ◽  
Protective Immunity ◽  
Specific Resistance ◽  
Epidemiologic Studies ◽  
Bcg Vaccine ◽  
Lung Pathology ◽  
Strain Specificity

ABSTRACT Recent preclinical and epidemiologic studies have suggested that certain Mycobacterium tuberculosis genotypes (in particular, Beijing lineage strains) may be resistant to Mycobacterium bovis BCG vaccine-induced antituberculosis protective immunity. To investigate the strain specificity of BCG-induced protective responses in a murine model of pulmonary tuberculosis, C57BL/6 mice were vaccinated with BCG vaccine and then challenged 2 months later with one of nine M. tuberculosis isolates. Four of these strains were from the W-Beijing lineage (HN878, N4, NHN5, and ChS) while four were non-Beijing-type isolates (C913, CDC1551, NY669, and NY920). As a control, the WHO standard M. tuberculosis Erdman strain was evaluated in these vaccination/challenge experiments. To assess the protective responses evoked by BCG immunization, organ bacterial burdens and lung pathology were assessed in vaccinated and naïve mice at 4, 12, and 20 weeks postchallenge as well as during the day of infection. At 4 weeks after the aerosol challenge with each of these strains, significantly reduced bacterial growth in the lungs and spleens and significantly improved lung pathology were seen in all vaccinated animals compared to naïve controls. After 12 weeks, reduced organ bacterial burdens were detected in vaccinated animals infected with six of nine challenge strains. Although lung CFU values were lower in vaccinated mice for only three of nine groups at 20 weeks postchallenge, significantly decreased lung inflammation was seen in all immunized animals relative to controls at 20 weeks postchallenge. Taken together, these data demonstrate that BCG vaccination protects against infection with diverse M. tuberculosis strains in the mouse model of pulmonary tuberculosis and suggest that strain-specific resistance to BCG-induced protective immunity may be uncommon.

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