Identification and Characterization of MFA1, the Gene Encoding Candida albicansa-Factor Pheromone

ABSTRACT In the opaque state, MTL a and MTLα strains of Candida albicans are able to mate, and this mating is directed by a pheromone-mediated signaling process. We have used comparisons of genome sequences to identify a C. albicans gene encoding a candidate a-specific mating factor. This gene is conserved in Candida dubliniensis and is similar to a three-gene family in the related fungus Candida parapsilosis but has extremely limited similarity to the Saccharomyces cerevisiae MFA1 (ScMFA1) and ScMFA2 genes. All these genes encode C-terminal CAAX box motifs characteristic of prenylated proteins. The C. albicans gene, designated CaMFA1, is found on chromosome 2 between ORF19.2165 and ORF19.2219. MFA1 encodes an open reading frame of 42 amino acids that is predicted to be processed to a 14-amino-acid prenylated mature pheromone. Microarray analysis shows that MFA1 is poorly expressed in opaque MTL a cells but is induced when the cells are treated with α-factor. Disruption of this C. albicans gene blocks the mating of MTL a cells but not MTLα cells, while the reintegration of the gene suppresses this cell-type-specific mating defect.

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Identification and characterization of YLR328W, theSaccharomyces cerevisiaestructural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme

FEBS Letters ◽

10.1016/s0014-5793(99)00852-2 ◽

1999 ◽

Vol 455 (1-2) ◽

pp. 13-17 ◽

Cited By ~ 39

Author(s):

Monica Emanuelli ◽

Francesco Carnevali ◽

Maria Lorenzi ◽

Nadia Raffaelli ◽

Adolfo Amici ◽

...

Keyword(s):

Recombinant Enzyme ◽

Gene Encoding ◽

Identification And Characterization

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Cloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B

Applied and Environmental Microbiology ◽

10.1128/aem.66.12.5480-5483.2000 ◽

2000 ◽

Vol 66 (12) ◽

pp. 5480-5483 ◽

Cited By ~ 16

Author(s):

Sean S. Dineen ◽

Marite Bradshaw ◽

Eric A. Johnson

Keyword(s):

Amino Acids ◽

Nucleotide Sequence ◽

Clostridium Botulinum ◽

Shuttle Vector ◽

Open Reading Frame ◽

Gene Encoding ◽

Reading Frame ◽

Heat Stable ◽

Group I

ABSTRACT Boticin B is a heat-stable bacteriocin produced byClostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene,btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing theHindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.

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Identification and characterization of cell type-specific and ubiquitous chromatin regulatory structures in the human genome

PLoS Genetics ◽

10.1371/journal.pgen.0030136.eor ◽

2005 ◽

Vol preprint (2007) ◽

pp. e136

Author(s):

Hualin Xi ◽

Hennady P Shulha ◽

Jane M Lin ◽

Teresa R Vales ◽

Yutao Fu ◽

...

Keyword(s):

Human Genome ◽

Cell Type ◽

Cell Type Specific ◽

Identification And Characterization

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Identification and characterization of cis-regulatory elements for photoreceptor type-specific transcription in zebrafish

10.1101/683284 ◽

2019 ◽

Author(s):

Wei Fang ◽

Yi Wen ◽

Xiangyun Wei

Keyword(s):

Core Promoter ◽

Regulatory Elements ◽

Specific Gene ◽

Protein Coding ◽

Core Promoters ◽

Protein Coding Genes ◽

The Core ◽

Cell Type Specific ◽

Identification And Characterization

AbstractTissue-specific or cell type-specific transcription of protein-coding genes is controlled by both trans-regulatory elements (TREs) and cis-regulatory elements (CREs). However, it is challenging to identify TREs and CREs, which are unknown for most genes. Here, we describe a protocol for identifying two types of transcription-activating CREs—core promoters and enhancers—of zebrafish photoreceptor type-specific genes. This protocol is composed of three phases: bioinformatic prediction, experimental validation, and characterization of the CREs. To better illustrate the principles and logic of this protocol, we exemplify it with the discovery of the core promoter and enhancer of the mpp5b apical polarity gene (also known as ponli), whose red, green, and blue (RGB) cone-specific transcription requires its enhancer, a member of the rainbow enhancer family. While exemplified with an RGB cone-specific gene, this protocol is general and can be used to identify the core promoters and enhancers of other protein-coding genes.

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Identification and characterization of the gene encoding an extracellular protease from haloarchaeon Halococcus salifodinae

Microbiological Research ◽

10.1016/j.micres.2020.126468 ◽

2020 ◽

Vol 236 ◽

pp. 126468 ◽

Cited By ~ 1

Author(s):

Jing Hou ◽

Dong Han ◽

Yao Zhou ◽

Yang Li ◽

Heng-Lin Cui

Keyword(s):

Extracellular Protease ◽

Gene Encoding ◽

Identification And Characterization

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Identification and characterization of a novel gene encoding myb-box binding zinc finger protein in Gossypium arboreum

Biologia Plantarum ◽

10.1007/s10535-012-0255-3 ◽

2012 ◽

Vol 56 (4) ◽

pp. 641-647 ◽

Cited By ~ 5

Author(s):

M. Zahur ◽

A. Maqbool ◽

M. Irfan ◽

A. Jamal ◽

N. Shahid ◽

...

Keyword(s):

Zinc Finger ◽

Zinc Finger Protein ◽

Gossypium Arboreum ◽

Gene Encoding ◽

Novel Gene ◽

Finger Protein ◽

Identification And Characterization

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Identification and Characterization of a Porcine Torovirus

Journal of Virology ◽

10.1128/jvi.72.5.3507-3511.1998 ◽

1998 ◽

Vol 72 (5) ◽

pp. 3507-3511 ◽

Cited By ~ 59

Author(s):

A. Kroneman ◽

L. A. H. M. Cornelissen ◽

M. C. Horzinek ◽

R. J. de Groot ◽

H. F. Egberink

Keyword(s):

Longitudinal Study ◽

Neutralizing Antibodies ◽

Rt Pcr ◽

Gene Encoding ◽

Nucleocapsid Gene ◽

Sequence Identity ◽

Young Pigs ◽

Porcine Torovirus ◽

Identification And Characterization

ABSTRACT A porcine torovirus (PoTV) was identified and characterized; it is a novel member of the genus Torovirus (familyCoronaviridae, order Nidovirales), closely related to but clearly distinct from the already recognized equine torovirus (ETV) and bovine torovirus (BoTV) representatives. Immunoelectron microscopy of feces from piglets revealed elongated, 120- by 55-nm particles which were recognized by a torovirus-specific antiserum. Amplification by reverse transcriptase (RT) PCR with primers designed to detect conserved regions (on the basis of the genomes of BoTV strain Breda and ETV strain Berne) resulted in the identification of the 489-bp nucleocapsid gene, encoding a 18.7-kDa protein. The sequence identity in this region between PoTV and both ETV and BoTV was only about 68%, whereas the latter two show 81% identity. Neutralizing antibodies directed against ETV were found in sera of adult and young pigs. In all 10 herds sampled, seropositive animals were present, and 81% of randomly selected adult sows possessed antibodies. A longitudinal study with RT PCR showed that piglets shed virus in the feces for 1 or more days, starting 4 to 14 days after weaning.

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