scholarly journals Closed Genome Sequence of Clostridium botulinum Strain CFSAN064329 (62A)

2018 ◽  
Vol 6 (26) ◽  
Author(s):  
Travis G. Wentz ◽  
Kuan Yao ◽  
Kristin M. Schill ◽  
N. Rukma Reddy ◽  
Guy E. Skinner ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Food Challenge ◽  
Strictly Anaerobic ◽  
Botulinum Neurotoxin A ◽  
Gram Positive ◽  
Content Type

Clostridium botulinum is a strictly anaerobic, Gram-positive, spore-forming bacterium that produces botulinum neurotoxin, a potent and deadly proteinaceous exotoxin. Clostridium botulinum strain CFSAN064329 (62A) produces an A1 serotype/subtype botulinum neurotoxin and is frequently utilized in food challenge and detection studies.

scholarly journals Draft Genome Sequence of a Clostridium botulinum Isolate from Thailand Harboring the Subtype bont/B8 Gene

2019 ◽  
Vol 8 (5) ◽  
Author(s):  
Jessica L. Halpin ◽  
Piyada Wangroongsarb ◽  
Chutima Jittaprasartsin ◽  
Janet K. Dykes ◽  
Carolina Lúquez
Keyword(s):  
Genome Sequence ◽  
Clostridium Botulinum ◽  
Botulinum Neurotoxin ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Type B ◽  
Content Type ◽  
Molecular Investigation ◽  
Foodborne Botulism

In 2010, a Clostridium botulinum type B isolate was recovered from fermented soybeans during a foodborne botulism investigation. Molecular investigation of the botulinum neurotoxin (bont) gene operon determined that the sequence was a new subtype, denoted B8.

scholarly journals First Insight into the Genome Sequence of Clostridium liquoris DSM 100320, a Butyrate- and Ethanol-Producing Bacterium

2018 ◽  
Vol 6 (18) ◽  
Author(s):  
Anja Poehlein ◽  
Hannes Neubauer ◽  
Philipp Niemeyer ◽  
Rolf Daniel
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Strictly Anaerobic ◽  
Gram Positive ◽  
Fermentation Products ◽  
Content Type ◽  
Protein Encoding ◽  
Encoding Genes ◽  
Insight Into

ABSTRACT Clostridium liquoris is a strictly anaerobic, Gram-positive, nonmotile, spore-forming, rod-shaped bacterium. The major fermentation products from glucose are ethanol and butyrate. C. liquoris was isolated from a 20-year-old liquor fermentation pit. The draft genome sequence consists of a chromosome (2.892 Mb) harboring 2,788 predicted protein-encoding genes.

scholarly journals Draft Genome Sequence of Clostridium tagluense Strain A121T, Isolated from a Permafrost Core in the Canadian High Arctic

2019 ◽  
Vol 8 (5) ◽  
Author(s):  
Takumi Murakami ◽  
Hiroshi Mori ◽  
Viktoria A. Shcherbakova ◽  
Yoshitaka Yoshimura ◽  
Takahiro Segawa
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
High Arctic ◽  
Draft Genome Sequence ◽  
Strictly Anaerobic ◽  
Canadian High Arctic ◽  
Gram Positive ◽  
Content Type ◽  
Psychrotolerant Bacterium

Clostridium tagluense strain A121T was isolated from a permafrost core in the Canadian High Arctic. This endospore-forming strain is a strictly anaerobic, Gram-positive, and psychrotolerant bacterium.

scholarly journals Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice

npj Vaccines ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Changjiao Gan ◽  
Wenbo Luo ◽  
Yunzhou Yu ◽  
Zhouguang Jiao ◽  
Sha Li ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Clostridium Botulinum ◽  
Freeze Drying ◽  
Botulinum Neurotoxin ◽  
Secretory Iga ◽  
Immune Protection ◽  
Dry Powder ◽  
Botulinum Neurotoxin A ◽  
Spray Freeze Drying

AbstractBotulinum neurotoxin (BoNT), produced by Clostridium botulinum, is generally known to be the most poisonous of all biological toxins. In this study, we evaluate the protection conferred by intratracheal (i.t.) inoculation immunization with recombinant Hc subunit (AHc) vaccines against aerosolized BoNT/A intoxication. Three AHc vaccine formulations, i.e., conventional liquid, dry powder produced by spray freeze drying, and AHc dry powder reconstituted in water are prepared, and mice are immunized via i.t. inoculation or subcutaneous (s.c.) injection. Compared with s.c.-AHc-immunized mice, i.t.-AHc-immunized mice exhibit a slightly stronger protection against a challenge with 30,000× LD50 aerosolized BoNT/A. Of note, only i.t.-AHc induces a significantly higher level of toxin-neutralizing mucosal secretory IgA (SIgA) production in the bronchoalveolar lavage of mice. In conclusion, our study demonstrates that the immune protection conferred by the three formulations of AHc is comparable, while i.t. immunization of AHc is superior to s.c. immunization against aerosolized BoNT/A intoxication.

scholarly journals Genome Sequence of Clostridium botulinum Strain Adk2012 Associated with a Foodborne Botulinum Case in Tottori, Japan, in 2012

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Hiroshi Asakura ◽  
Shiori Yamamoto ◽  
Yoshika Momose ◽  
Haru Kato ◽  
Masaaki Iwaki ◽  
...  
Keyword(s):  
Genome Size ◽  
Genome Sequence ◽  
Clostridium Botulinum ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Foodborne Botulism

ABSTRACT We report here a draft genome sequence of Clostridium botulinum Adk2012 responsible for a foodborne botulism case that occurred in Tottori, Japan, in 2012. Its genome size was 2,904,173 bp, with 46 rRNAs and 54 tRNAs, at a coverage of 14.5×.

scholarly journals Complete Genome Sequence of Bacillus cereus Sensu Stricto VKM B-370, Isolated from the Silkworm Bombyx mori

2021 ◽  
Vol 10 (20) ◽  
Author(s):  
Emma G. Piligrimova ◽  
Rustam M. Buzikov ◽  
Olesya A. Kazantseva ◽  
Andrey M. Shadrin
Keyword(s):  
Bacillus Cereus ◽  
Bombyx Mori ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sensu Stricto ◽  
Host Strain ◽  
Gram Positive ◽  
Content Type ◽  
Silkworm Bombyx Mori

ABSTRACT Bacillus cereus is a Gram-positive rod-shaped spore-forming bacterium widespread in different environmental niches. Here, we report the complete genome sequence of Bacillus cereus VKM B-370 from the All-Russian Collection of Microorganisms, the host strain for bacteriophages vB_BtS_B83, vB_BcM_Sam46, vB_BcM_Sam112, and Izhevsk.

scholarly journals Finished Whole-Genome Sequence of Clostridium argentinense Producing Botulinum Neurotoxin Type G

2017 ◽  
Vol 5 (21) ◽  
Author(s):  
Jessica L. Halpin ◽  
Karen Hill ◽  
Shannon L. Johnson ◽  
David Carlton Bruce ◽  
T. Brian Shirey ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Botulinum Neurotoxin ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Reference Quality

ABSTRACT Here, we present a closed genome sequence for Clostridium argentinense strain 89G, the first strain identified to produce botulinum neurotoxin type G (BoNT/G). Although discovered in 1970, to date, there have been no reference quality sequences publicly available for this species.

scholarly journals Draft Genome Sequence ofRobinsoniella peoriensis6600698, a Confounder ofClostridium difficileDiagnosis

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Chrissy h. Roberts ◽  
Helen Alexandra Shaw ◽  
Natalie Ferguson ◽  
Mark Holland ◽  
Brendan W. Wren ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Positive Result ◽  
Genome Sequence ◽  
Clinical Symptoms ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Potential Confounder ◽  
Strictly Anaerobic ◽  
Gram Positive ◽  
Selective Agar

Robinsoniella peoriensisis a Gram-positive, strictly anaerobic, spore-forming, rod-shaped organism. Here, we report the draft genome ofR. peoriensis6600698, initially classified asClostridium difficiledue to growth on selective agar, a fecalgdhPCR-positive result, and clinical symptoms.R. peoriensisis a potential confounder ofC. difficilediagnosis.

scholarly journals Draft Genome Sequence of Lactobacillus crispatus UMB1163, Isolated from the Female Urinary Tract

2020 ◽  
Vol 9 (23) ◽  
Author(s):  
Fabeiha Khan ◽  
Taylor Miller-Ensminger ◽  
Adelina Voukadinova ◽  
Alan J. Wolfe ◽  
Catherine Putonti
Keyword(s):  
Urinary Tract ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Vaginal Infections ◽  
Content Type ◽  
Lactobacillus Crispatus ◽  
Female Urinary Tract

ABSTRACT Lactobacillus crispatus is a Gram-positive bacterium shown to protect against urinary and vaginal infections. Here, we report the draft genome sequence of L. crispatus UMB1163, isolated from the female urinary tract.

scholarly journals Draft Genome Sequence of Bacillus marisflavi CK-NBRI-03, Isolated from Agricultural Soil

2020 ◽  
Vol 9 (7) ◽  
Author(s):  
Mayank Gupta ◽  
Puneet Singh Chauhan ◽  
Sudhir K. Sopory ◽  
Sneh L. Singla-Pareek ◽  
Nidhi Adlakha ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Agricultural Soil ◽  
Draft Genome ◽  
Uttar Pradesh ◽  
Draft Genome Sequence ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Content Type ◽  
Draft Genome Assembly

Here, we report the 4.34-Mb draft genome assembly of Bacillus marisflavi CK-NBRI-03 (or P3), a Gram-positive bacterium, with an average G+C content of 48.66%. P3 was isolated from agricultural soil from the Badaun (midwestern plain zone) region of Uttar Pradesh, India.

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