We report the draft genome sequence ofLactococcus lactissubsp.lactisbv.diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina.L. lactissubsp.lactisbv.diacetylactis is one of the most important microorganisms used as starter culture around the world. The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds (diacetyl, acetoin, and 2,3-butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes ofL. lactisbv. diacetylactis strains.