scholarly journals Genome Sequence of Bibersteinia trehalosi Strain Y31 Isolated from the Pneumonic Lung of a Bighorn Sheep

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Abirami Kugadas ◽  
Jodi L. Humann ◽  
Sebastián Aguilar Pierlé ◽  
Subramaniam Srikumaran ◽  
Kelly A. Brayton
Keyword(s):  
Genome Sequence ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Mannheimia Haemolytica ◽  
Dependent Inhibition ◽  
Bibersteinia Trehalosi

Here, we report the genome sequence for Bibersteinia trehalosi strain Y31, isolated from the lungs of a bighorn sheep ( Ovis canadensis ) that had succumbed to pneumonia, which exhibits proximity-dependent inhibition (PDI) of Mannheimia haemolytica . The sequence will be used to understand the mechanism of PDI for these organisms.

scholarly journals Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida

2012 ◽  
Vol 78 (18) ◽  
pp. 6683-6688 ◽  
Author(s):  
Jegarubee Bavananthasivam ◽  
Rohana P. Dassanayake ◽  
Abirami Kugadas ◽  
Sudarvili Shanthalingam ◽  
Douglas R. Call ◽  
...  
Keyword(s):  
Pore Size ◽  
Pasteurella Multocida ◽  
Ovis Canadensis ◽  
Mannheimia Haemolytica ◽  
Experimental Conditions ◽  
Content Type ◽  
Inhibition Of Growth ◽  
Dependent Inhibition ◽  
Bibersteinia Trehalosi

ABSTRACTMannheimia haemolytica,Pasteurella multocida, andBibersteinia trehalosihave been identified in the lungs of pneumonic bighorn sheep (BHS;Ovis canadensis). Of these pathogens,M. haemolyticahas been shown to consistently cause fatal pneumonia in BHS under experimental conditions. However,M. haemolyticahas been isolated by culture less frequently than the other bacteria. We hypothesized that the growth ofM. haemolyticais inhibited by other bacteria in the lungs of BHS. The objective of this study was to determine whetherP. multocidainhibits the growth ofM. haemolytica. Although in monoculture both bacteria exhibited similar growth characteristics, in coculture withP. multocidathere was a clear inhibition of growth ofM. haemolytica. The inhibition was detected at mid-log phase and continued through the stationary phase. When cultured in the same medium, the growth ofM. haemolyticawas inhibited when both bacteria were separated by a membrane that allowed contact (pore size, 8.0 μm) but not when they were separated by a membrane that limited contact (pore size, 0.4 μm). Lytic bacteriophages or bactericidal compounds could not be detected in the culture supernatant fluid from monocultures ofP. multocidaor fromP. multocida-M. haemolyticacocultures. These results indicate thatP. multocidainhibits the growth ofM. haemolyticaby a contact- or proximity-dependent mechanism. If the inhibition of growth ofM. haemolyticabyP. multocidaoccursin vivoas well, it could explain the inconsistent isolation ofM. haemolyticafrom the lungs of pneumonic BHS.

scholarly journals Harnessing cross-species alignment to discover SNPs and generate a draft genome sequence of a bighorn sheep (Ovis canadensis)

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Joshua M Miller ◽  
Stephen S Moore ◽  
Paul Stothard ◽  
Xiaoping Liao ◽  
David W Coltman
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Draft Genome Sequence

scholarly journals A Multivalent Mannheimia-Bibersteinia Vaccine Protects Bighorn Sheep against Mannheimia haemolytica Challenge

2011 ◽  
Vol 18 (10) ◽  
pp. 1689-1694 ◽  
Author(s):  
Renuka Subramaniam ◽  
Sudarvili Shanthalingam ◽  
Jegarubee Bavananthasivam ◽  
Abirami Kugadas ◽  
Kathleen A. Potter ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Population Decline ◽  
Surface Antigens ◽  
Bighorn Sheep ◽  
Mannheimia Haemolytica ◽  
Domestic Sheep ◽  
Content Type ◽  
The Mean ◽  
Bibersteinia Trehalosi ◽  
Observation Period

ABSTRACTBighorn sheep (BHS) are more susceptible than domestic sheep (DS) toMannheimia haemolyticapneumonia. Although both species carryM. haemolyticaas a commensal bacterium in the nasopharynx, DS carry mostly leukotoxin (Lkt)-positive strains while BHS carry Lkt-negative strains. Consequently, antibodies to surface antigens and Lkt are present at much higher titers in DS than in BHS. The objective of this study was to determine whether repeated immunization of BHS with multivalentMannheimia-Bibersteiniavaccine will protect them uponM. haemolyticachallenge. Four BHS were vaccinated with a culture supernatant vaccine prepared fromM. haemolyticaserotypes A1 and A2 andBibersteinia trehalosiserotype T10 on days 0, 21, 35, 49, and 77. Four other BHS were used as nonvaccinated controls. On the day of challenge, 12 days after the last immunization, the mean serum titers of Lkt-neutralizing antibodies and antibodies to surface antigens againstM. haemolyticawere 1:160 and 1:4,000, respectively. Following intranasal challenge withM. haemolyticaA2 (1 × 105CFU), all four control BHS died within 48 h. Necropsy revealed acute fibrinonecrotic pneumonia characteristic ofM. haemolyticainfection. None of the vaccinated BHS died during the 8 weeks postchallenge observation period. Radiography at 3 weeks postchallenge revealed no lung lesions in two vaccinated BHS and mild lesions in the other two, which resolved by 8 weeks postchallenge. These results indicate that if BHS can be induced to develop high titers of Lkt-neutralizing antibodies and antibodies to surface antigens, they are likely to surviveM. haemolyticachallenge which is likely to reduce the BHS population decline due to pneumonia.

scholarly journals Brucellosis in Captive Rocky Mountain Bighorn Sheep (Ovis canadensis) Caused by Brucella abortus Biovar 4

2004 ◽  
Vol 40 (2) ◽  
pp. 311-315 ◽  
Author(s):  
Terry J. Kreeger ◽  
Walter E. Cook ◽  
William H. Edwards ◽  
Todd Cornish
Keyword(s):  
Brucella Abortus ◽  
Rocky Mountain ◽  
Bighorn Sheep ◽  
Ovis Canadensis

scholarly journals Desert Bighorn Sheep: Changes in Genetic Variation Over Time and the Impact of Merging Populations

2014 ◽  
Vol 5 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Philip W. Hedrick ◽  
John D. Wehausen
Keyword(s):  
Genetic Variation ◽  
New Mexico ◽  
Monitoring And Evaluation ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Mexican Population ◽  
Desert Bighorn Sheep ◽  
Two Generations ◽  
The Impact ◽  
Over Time

Abstract Founder effects, genetic bottlenecks, and genetic drift in general can lead to low levels of genetic diversity, which can influence the persistence of populations. We examine genetic variation in two populations of desert bighorn sheep Ovis canadensis from New Mexico and Mexico to measure change over time and evaluate the impact of introducing individuals from one population into the other. Over about three generations, the amount of genetic variation in the New Mexico population increased. In contrast, over about two generations the amount of genetic variation in the Mexican population decreased by a great extent compared with an estimate from another Mexican population from which it is primarily descended. The potential reasons for these changes are discussed. In addition, although both populations have low genetic variation, introduction of Mexican rams into the New Mexico population might increase the amount of genetic variation in the New Mexico population. Overall, it appears that management to increase genetic variation might require substantial detailed monitoring and evaluation of ancestry from the different sources and fitness components.

Factors influencing heart rate in free-ranging bighorn sheep: a physiological approach to the study of wildlife harassment

1979 ◽  
Vol 57 (10) ◽  
pp. 2010-2021 ◽  
Author(s):  
Robert A. MacArthur ◽  
Ronald H. Johnston ◽  
Valerius Geist
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Activity Level ◽  
Base Line ◽  
Vehicular Traffic ◽  
Behavioral Reactions ◽  
Factors Influencing ◽  
Close Range ◽  
Physiological Approach ◽  
Free Ranging

The telemetered heart rates (HR) of unrestrained female bighorn sheep (Ovis canadensis canadensis) were recorded under various behavioral and environmental circumstances. In all ewes HR varied positively with activity level and inversely with distance to a road traversing the study area. The HR recorded from animals moving at night or through timber by day were higher than during daytime movement across open slopes.Responses to transient stimuli varied greatly. The appearance of free-ranging canids evoked maximal increases in HR in all ewes. Vehicular traffic and aircraft elicited HR responses only at close range (< 200 m). Most (78.1%) HR responses to disturbing stimuli preceded or occurred in the absence of overt behavioral reactions. The HR usually peaked within 60 s of the onset of the response and recovered to predisturbance base line in less than 200 s. The appearance and continued presence (1–10 min) of a human within 50 m of the sheep resulted in a 20% rise in mean HR. The significance of these findings is discussed in relation to the behavior, ecology, and bioenergetics of bighorn sheep.

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