scholarly journals Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken

2017 ◽  
Vol 5 (47) ◽  
Author(s):  
George C. Paoli ◽  
Chandi Wijey ◽  
Ly-Huong Nguyen ◽  
Chin-Yi Chen ◽  
Xianghe Yan ◽  
...  

ABSTRACT Brochothrix thermosphacta is an important meat spoilage bacterium. Here we report the genome sequences of two strains of B. thermosphacta isolated from ground chicken. The genome sequences were determined using long-read PacBio single-molecule real-time (SMRT) technology and are the first complete genome sequences reported for B. thermosphacta.

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Luis F. Espinosa-Camacho ◽  
Gabriela Delgado ◽  
Guadalupe Miranda-Novales ◽  
Gloria Soberón-Chávez ◽  
Luis D. Alcaraz ◽  
...  

ABSTRACT Two Pseudomonas aeruginosa strains isolated from children with bacteremia in Mexico City were sequenced using PacBio RS-II single-molecule real-time (SMRT) technology. The strains consist of a 7.0- to 7.4-Mb chromosome, with a high content of mobile elements, and variation in the genetic content of class 1 integron In1409.


2017 ◽  
Vol 5 (38) ◽  
Author(s):  
Christoph Jans ◽  
Sandra Lagler ◽  
Christophe Lacroix ◽  
Leo Meile ◽  
Marc J. A. Stevens

ABSTRACT The genomes of Lactobacillus curvatus KG6, L. curvatus MRS6, and Lactobacillus sakei FAM18311 were sequenced and assembled using PacBio single-molecule real-time (SMRT) technology. The strains were isolated from Swiss fermented meat products. Circular chromosomes were of 1.98 Mbp (KG6), 2.11 Mbp (MRS6), and 1.95 Mbp (FAM18311), with a G+C content of 41.3 to 42.0%.


2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Håkon Kaspersen ◽  
Thomas H. A. Haverkamp ◽  
Hanna Karin Ilag ◽  
Øivind Øines ◽  
Camilla Sekse ◽  
...  

ABSTRACT In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.


2020 ◽  
Vol 9 (3) ◽  
Author(s):  
Hallie E. Rauch ◽  
Julie Haendiges ◽  
Maria Balkey ◽  
Maria Hoffmann

We report here the closed genomes of Salmonella enterica strains from the 2017–2018 multistrain, multistate kratom outbreak using single-molecule real-time DNA sequencing. Four of the genomes consist of one circular chromosome, and the fifth has a circular chromosome and a single plasmid.


2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Raffael C. Inglin ◽  
Leo Meile ◽  
Jochen Klumpp ◽  
Marc J. A. Stevens

ABSTRACT We present here the complete genome sequence of Lactobacillus plantarum RI-113, a strain isolated from salami, which was determined using single-molecule real-time sequencing.


2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Lakshmi Chandrasekaran ◽  
Daniela I. Drautz-Moses ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
Anthony Wong ◽  
...  

Citricoccus sp. strain SGAir0253 was isolated from indoor air collected in Singapore. Its genome sequence was assembled using single-molecule real-time sequencing. It comprises one chromosome of 3.32 Mb and two plasmids of 137 kb and 99 kb. The genome consists of 2,950 protein-coding genes, 49 tRNAs, and 9 rRNAs.


2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Priya DasSarma ◽  
Brian P. Anton ◽  
Satyajit L. DasSarma ◽  
Fabiana L. Martinez ◽  
Daniel Guzman ◽  
...  

Two extremely halophilic archaea, namely, Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, were isolated from a Bolivian salt mine and their genomes sequenced using single-molecule real-time sequencing. The GC-rich genomes of BOL5-4 and BOL6-1 were 4.6 and 3.8 Mbp, respectively, with large chromosomes and multiple megaplasmids. Genome annotation was incorporated into HaloWeb and methylation patterns incorporated into REBASE.


2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Akino Shiroma ◽  
Yasunobu Terabayashi ◽  
Kazuma Nakano ◽  
Makiko Shimoji ◽  
Hinako Tamotsu ◽  
...  

2021 ◽  
Vol 10 (34) ◽  
Author(s):  
Alexey Fomenkov ◽  
Priya DasSarma ◽  
Sean P. Kennedy ◽  
Richard J. Roberts ◽  
Shiladitya DasSarma

The genome of halophilic archaeon Haloarcula sinaiiensis ATCC 33800 was sequenced and assembled and comprises seven replicons. Four m6A and one m4C modified motifs and their responsible methyltransferase genes have been identified in the genome by single-molecule real-time (SMRT) sequencing and bioinformatic analysis.


2017 ◽  
Vol 5 (13) ◽  
Author(s):  
Ni Putu Desy Aryantini ◽  
Jashbhai B. Prajapati ◽  
Tadasu Urashima ◽  
Kenji Fukuda

ABSTRACT Lactobacillus fermentum MTCC 25067 (formerly TDS030603) is capable of producing a highly viscous slime exopolysaccharide. We report here the complete genome sequence of the strain, which was deciphered by using PacBio single-molecule real-time sequencing technology.


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