scholarly journals Genome Sequence of Trypanosoma cruzi Strain Bug2148

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Francisco Callejas-Hernández ◽  
Núria Gironès ◽  
Manuel Fresno
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Vertical Transmission ◽  
Genome Sequence ◽  
Genomic Sequence ◽  
Gene Organization ◽  
Polycistronic Transcription ◽  
Complex Mechanisms ◽  
Plastic Genome

ABSTRACT Trypanosoma cruzi belongs to the group of mitochondrion-containing eukaryotes and has a highly plastic genome, unusual gene organization, and complex mechanisms for gene expression (polycistronic transcription). We report here the genome sequence of strain Bug2148, the first genomic sequence belonging to cluster TcV, which has been related to vertical transmission.

scholarly journals Epigenetic Regulation of Polymerase II Transcription Initiation in Trypanosoma cruzi: Modulation of Nucleosome Abundance, Histone Modification, and Polymerase Occupancy by O-Linked Thymine DNA Glucosylation

2011 ◽  
Vol 10 (11) ◽  
pp. 1465-1472 ◽  
Author(s):  
Dilrukshi Ekanayake ◽  
Robert Sabatini
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Epigenetic Regulation ◽  
Chromatin Structure ◽  
Transcription Initiation ◽  
Gene Promoter ◽  
Gene Organization ◽  
Promoter Regions ◽  
Pol Ii ◽  
Content Type

ABSTRACT Very little is understood regarding how transcription is initiated/regulated in the early-diverging eukaryote Trypanosoma cruzi . Unusually for a eukaryote, genes transcribed by RNA polymerase (Pol) II in T. cruzi are arranged in polycistronic transcription units (PTUs). On the basis of this gene organization, it was previously thought that trypanosomes rely solely on posttranscriptional processes to regulate gene expression. We recently localized a novel glucosylated thymine DNA base, called base J, to potential promoter regions of PTUs throughout the trypanosome genome. Loss of base J, following the deletion of JBP1, a thymidine hydroxylase involved with synthesis, led to a global increase in the Pol II transcription rate and gene expression. In order to determine the mechanism by which base J regulates transcription, we have characterized changes in chromatin structure and Pol II recruitment to promoter regions following the loss of base J. The loss of base J coincides with a decrease in nucleosome abundance, increased histone H3/H4 acetylation, and increased Pol II occupancy at promoter regions, including the well-characterized spliced leader RNA gene promoter. These studies present the first direct evidence for epigenetic regulation of Pol II transcription initiation via DNA modification and chromatin structure in kinetoplastids as well as provide a mechanism for regulation of trypanosome gene expression via the novel hypermodified base J.

scholarly journals Completion of the Genome Sequence of Brucella abortus and Comparison to the Highly Similar Genomes of Brucella melitensis and Brucella suis

2005 ◽  
Vol 187 (8) ◽  
pp. 2715-2726 ◽  
Author(s):  
Shirley M. Halling ◽  
Brooke D. Peterson-Burch ◽  
Betsy J. Bricker ◽  
Richard L. Zuerner ◽  
Zhang Qing ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Brucella Abortus ◽  
Genomic Sequence ◽  
Brucella Melitensis ◽  
Outer Membrane Proteins ◽  
Field Isolate ◽  
Gene Organization ◽  
Nucleotide Polymorphisms ◽  
Brucella Suis ◽  
Complete Genomic

ABSTRACT Brucellosis is a worldwide disease of humans and livestock that is caused by a number of very closely related classical Brucella species in the alpha-2 subdivision of the Proteobacteria. We report the complete genome sequence of Brucella abortus field isolate 9-941 and compare it to those of Brucella suis 1330 and Brucella melitensis 16 M. The genomes of these Brucella species are strikingly similar, with nearly identical genetic content and gene organization. However, a number of insertion-deletion events and several polymorphic regions encoding putative outer membrane proteins were identified among the genomes. Several fragments previously identified as unique to either B. suis or B. melitensis were present in the B. abortus genome. Even though several fragments were shared between only B. abortus and B. suis, B. abortus shared more fragments and had fewer nucleotide polymorphisms with B. melitensis than B. suis. The complete genomic sequence of B. abortus provides an important resource for further investigations into determinants of the pathogenicity and virulence phenotypes of these bacteria.

Transcriptional and Post-Transcriptional Control of Tubulin Gene Expression in Trypanosoma cruzi

1992 ◽  
Vol 11 (2) ◽  
pp. 101-109 ◽  
Author(s):  
TURÁN P. ÜRMÉNYI ◽  
FIRMINO T. DE CASTRO ◽  
JOSÉ FRANCISCO O. CARVALHO ◽  
WANDERLEY DE SOUZA ◽  
EDSON RONDINELLI
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Transcriptional Control ◽  
Tubulin Gene

scholarly journals Complete Genome Sequence of a Vampire Bat Rabies Virus from French Guiana

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Anne Lavergne ◽  
Edith Darcissac ◽  
Hervé Bourhy ◽  
Sourakhata Tirera ◽  
Benoît de Thoisy ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
French Guiana ◽  
Genomic Sequence ◽  
Amazon Region ◽  
Desmodus Rotundus ◽  
Bat Rabies ◽  
Vampire Bat

A rabies virus was detected in a common vampire bat ( Desmodus rotundus ) in French Guiana. Its genomic sequence was obtained and found to be closely related to other hematophagous bat-related viruses that widely circulate in the northern Amazon region. This virus is named AT6.

scholarly journals Mother-child transmission of Chagas disease: could coinfection with human immunodeficiency virus increase the risk?

2009 ◽  
Vol 42 (2) ◽  
pp. 107-109 ◽  
Author(s):  
Pablo Gustavo Scapellato ◽  
Edgardo Gabriel Bottaro ◽  
María Teresa Rodríguez-Brieschke
Keyword(s):  
Human Immunodeficiency Virus ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Vertical Transmission ◽  
Disease Transmission ◽  
Transmission Rate ◽  
Buffy Coat ◽  
Child Transmission ◽  
Serological Tests ◽  
Immunodeficiency Virus

A study was conducted on all newborns from mothers with Chagas disease who were attended at Hospital Donación F. Santojanni between January 1, 2001, and August 31, 2007. Each child was investigated for the presence of Trypanosoma cruzi parasitemia through direct examination of blood under the microscope using the buffy coat method on three occasions during the first six months of life. Serological tests were then performed. Ninety-four children born to mothers infected with Trypanosoma cruzi were attended over the study period. Three of these children were born to mothers coinfected with the human immunodeficiency virus. Vertical transmission of Chagas disease was diagnosed in 13 children, in all cases by identifying parasitemia. The overall Chagas disease transmission rate was 13.8% (13/94). It was 100% (3/3) among the children born to mothers with HIV infection and 10.9% (10/91) among children born to mothers without HIV [Difference = 0.89; CI95 = 0.82-0.95; p = 0.0021]. We concluded that coinfection with HIV could increase the risk of vertical transmission of Chagas disease.

scholarly journals First draft genome sequence of a strain belonging to the Zoogloea genus and its gene expression in situ

2017 ◽  
Vol 12 (1) ◽  
Author(s):  
Emilie E. L. Muller ◽  
Shaman Narayanasamy ◽  
Myriam Zeimes ◽  
Cédric C. Laczny ◽  
Laura A. Lebrun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Its Gene
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