scholarly journals Genetic control of cell-mediated immunity in rats: involvement of RT1.B locus determinants in the proliferative response of T lymphocytes to Listeria antigens.

1982 ◽  
Vol 38 (2) ◽  
pp. 521-529 ◽  
Author(s):  
T W Jungi ◽  
R Jungi
Keyword(s):  
T Lymphocytes ◽  
Genetic Control ◽  
Proliferative Response ◽  
Cell Mediated Immunity ◽  
B Locus

scholarly journals Private specificities of H-2K and H-2D loci as possible selective targets for effector lymphocytes in cell-mediated immunity.

1975 ◽  
Vol 141 (1) ◽  
pp. 11-26 ◽  
Author(s):  
B D Brondz ◽  
I K Egorov ◽  
G I Drizlikh
Keyword(s):  
T Lymphocytes ◽  
Target Cells ◽  
Cell Mediated Immunity ◽  
Skin Grafts ◽  
Immune Lymphocytes ◽  
Direct Cytotoxicity ◽  
Cross Absorption ◽  
Private Specificity ◽  
Two Populations

Receptors of effector T lymphocytes of congeneic strains of mice do not recognize public H-2 specificities and react to private H-2 specificities only. This has been established with the use of three tests: direct cytotoxicity assay of immune lymphocytes upon target cells, specific absorption of the lymphocytes on the target cells, and rejection of skin grafts at an accelerated fashion. Immunization with two private H-2 specificities in the system C57BL/10ScSn leads to B10.D2 induces formation of two corresponding populations of effector lymphocytes in unequal proportion: a greater part of them is directed against the private specificity H-2.33 (Kb), while the smaller part is towards H-2.2 (Db) private specificity. These two populations of effector lymphocytes do not overlap, as demonstrated by experiments on their cross-absorption on B10.D2 (R107), B10.D2 (R101), B10.A(2R), and B10.A(5R) target cells, as well as on mixtures of R107 and R101 targets. Following removal of lymphocytes reacting with one of the private H-2 specificities, lymphocytes specific to the other specificity are fully maintained. A mixture of target cells, each bearing one of the two immunizing private specificities, absorbs 100% of the immune lymphocytes and is totally destroyed by them. It is suggested that H-2 antigens are natural complexes of hapten-carrier type, in which the role of hapten is played by public H-2 specifities and that of the carrier determinant by either private H-2 specificities or structures closely linked to them. Various models of steric arrangement of MHC determinants recognized by receptors of effector T lymphocytes are discussed.

Expanded CD4+CD45RO+ phenotype and defective proliferative response in T lymphocytes from patients with Crohn's disease

1996 ◽  
Vol 110 (4) ◽  
pp. 1008-1019 ◽  
Author(s):  
LI Roman ◽  
L Manzano ◽  
A De La Hera ◽  
L Abreu ◽  
I Rossi ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
T Lymphocytes ◽  
Crohn's Disease ◽  
Proliferative Response

scholarly journals Transplantation for severe combined immunodeficiency with HLA-A,B,D,DR incompatible parental marrow cells fractionated by soybean agglutinin and sheep red blood cells

Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 341-348 ◽  
Author(s):  
Y Reisner ◽  
N Kapoor ◽  
D Kirkpatrick ◽  
MS Pollack ◽  
S Cunningham-Rundles ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Blood Cells ◽  
Severe Combined Immunodeficiency ◽  
Cell Mediated Immunity ◽  
Combined Immunodeficiency ◽  
Soybean Agglutinin ◽  
Graft Versus Host ◽  
The Third ◽  
Immunologic Reconstitution ◽  
Marrow Cells

Abstract Three patients with severe combined immunodeficiency (SCID) received transplants of HLA haplotype-mismatched parental bone marrow depleted of T lymphocytes by differential agglutination with soybean agglutinin (SBA) and subsequent E-rosette depletion. Two patients achieved durable engraftment with reconstitution of both humoral and cell-mediated immunity. Neither of these patients developed graft versus host disease (GVHD). The third patient achieved only a transient engraftment with concomitant development of mitogen-responsive lymphocytes of paternal origin. Our experience indicates that depletion of T lymphocytes by this technique can abrogate the potential of histoincompatible marrow grafts to induce lethal GVHD without limiting immunologic reconstitution. It also provides further evidence of nonimmune mechanisms of graft resistance that may necessitate preparative treatment of patients with SCID before transplantation with HLA- mismatched marrow cells.

scholarly journals Development of suppressor T lymphocytes for Epstein-Barr virus-induced B-lymphocyte outgrowth during acute infectious mononucleosis: assessment by two quantitative systems

Blood ◽  
1981 ◽  
Vol 57 (3) ◽  
pp. 510-517 ◽  
Author(s):  
RT Schooley ◽  
BF Haynes ◽  
J Grouse ◽  
C Payling-Wright ◽  
AS Fauci ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Epstein Barr Virus ◽  
B Lymphocyte ◽  
Acute Stage ◽  
Cell Mediated Immunity ◽  
Barr Virus ◽  
Epstein Barr

Abstract A system of 3H-thymidine incorporation by lymphocytes in culture for 3 wk has been utilized for quantitative assessment of the ability of T lymphocytes to inhibit outgrowth of autologous Epstein-Barr virus (EBV) transformed B lymphocytes. Lymphocytes from EBV-seronegative individuals lack the ability to suppress outgrowth of autologous EBV- transformed B lymphocytes. This capability appears during the course of primary EBV-induced infectious mononucleases (IM) as the atypical lymphocytosis is subsiding and persists for years after recovery from primary EBV infection. The ability of T lymphocytes from EBV- seropositive subjects or convalescent IM patients to inhibit B- lymphocyte outgrowth is not HLA restricted. Thus, T lymphocytes capable of inhibition of in vitro EBV-induced B-cell outgrowth emerge during the acute stage of IM and may represent an important control mechanism of EBV-induced B-lymphocyte proliferation in vivo. The system provides a highly sensitive quantitative means for in vitro assessment of cell- mediated immunity to EBV.

Proliferatíve Response of T Lymphocytes to Mitogenic Lectins in Essential Hypertension

Nephron ◽  
1991 ◽  
Vol 58 (4) ◽  
pp. 413-417 ◽  
Author(s):  
Shaul M. Shasha ◽  
Uri Shasha ◽  
Batya Kristal ◽  
Miriam Barzilai ◽  
Osnat Steinberger ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
T Lymphocytes ◽  
Proliferative Response

scholarly journals Effect of a thymic factor on T lymphocytes in B cell chronic lymphocytic leukemia: in vitro and in vivo studies

Blood ◽  
1984 ◽  
Vol 64 (3) ◽  
pp. 667-671 ◽  
Author(s):  
F Lauria ◽  
D Raspadori ◽  
S Tura
Keyword(s):  
T Cell ◽  
Chronic Lymphocytic Leukemia ◽  
T Lymphocytes ◽  
B Cell ◽  
Proliferative Response ◽  
Lymphocytic Leukemia ◽  
Before And After ◽  
Cell Chronic Lymphocytic Leukemia

Abstract Abnormalities of T lymphocytes in B cell chronic lymphocytic leukemia (B-CLL) have been extensively documented by several immunologic investigations. Following recent studies pointing to the favorable effect of TP-1, a partially purified extract of calf thymus, on the T cell-mediated immunity of several diseases, including Hodgkin's disease, we have used monoclonal antibodies and the enriched T lymphocytes of 16 untreated B-CLL patients to evaluate the proportion of T cell subsets before and after the administration of TP-1. In addition, the proliferative response to phytohemagglutinin (PHA) and the helper function in a pokeweed mitogen (PWM) system were assessed. In ten cases, the effect of TP-1 was also studied in vitro by evaluating the same parameters before and after incubation of B-CLL T cells with the drug. The study demonstrated that in vivo administration of TP-1 increases significantly (P less than .001) the proportion of the defective helper/inducer T cell population (OKT4-positive cells) in B-CLL, leading to a near normal OKT4/OKT8 ratio. Furthermore, the improved phenotypic profile was accompanied by an increased proliferative response to PHA and, in particular, by a significant increase (P less than .01) of T helper capacity; this increase was, however, insufficient to enable the normalization of the serum immunoglobulin levels. The in vitro incubation of B-CLL T lymphocytes did not succeed in producing significant modifications in distribution and function.

scholarly journals Lymphocyte transformation induced by autologous cells. V. generation of immunologic memory and specificity during the autologous mixed lymphocyte reaction

1977 ◽  
Vol 146 (6) ◽  
pp. 1833-1838 ◽  
Author(s):  
ME Weksler ◽  
R Kozak
Keyword(s):  
T Lymphocytes ◽  
Lymphocyte Proliferation ◽  
Mixed Lymphocyte Reaction ◽  
Protein A ◽  
Lymphocyte Transformation ◽  
Cell Mediated Immunity ◽  
Staphylococcal Protein A ◽  
Autologous Mixed Lymphocyte Reaction ◽  
Proliferation In Vitro

Lymphocyte proliferation in vitro may follow antigen recognition and serve as a correlate of cell-mediated immunity. Lymphocyte proliferation can also be simulated by nonimmune mechanisms as, for example, following culture with plant lectin, lipopolysaccharides, or staphylococcal protein A (1). The autologous mixed lymphocyte reaction (MLR) refers to the proliferation of T lymphocytes cultured with autologous mon-T lymphocytes (2,3). The purpose of this study was to determine whether lymphocyte proliferation in the autologous MLR results from immune or nonimmune mechanisms. We have shown that the autologous MLR has two classical attributes of an immune phenomenon: memory and specificity.

scholarly journals Properties of reticulum cell sarcomas in SJL/J mice. V. Nature of reticulum cell sarcoma surface antigen which induces proliferation of normal SJL/J T cells.

1977 ◽  
Vol 146 (1) ◽  
pp. 132-145 ◽  
Author(s):  
N M Ponzio ◽  
C S David ◽  
D C Shreffler ◽  
G J Thorbecke
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Tumor Cells ◽  
Proliferative Response ◽  
Reticulum Cell ◽  
Reticulum Cell Sarcoma ◽  
Cell Surface Antigens ◽  
Cell Sarcoma ◽  
Lymph Node Cells

The results of studies on the reticulum cell sarcoma (RCS) tumors of SJL/J mice presented here, indicate that spontaneous tumors, which arise in older mice, also possess the capacity to induce the vigorous proliferative response in syngenetic T lymphocytes that are characteristic of the transplantable RCS lines. Analysis of cell surface antigens revealed the presence of Ia determinats on gradient-purified transplantable RCS tumor cells; however, these cells did not express Thy 1.2, nIg, or, any of the viral proteins that were tested for by specific antisera. Pretreatment of RCS cells with anti-Ia sera and complement-deleted cells that were stimulatory for syngenetic T lymphocytes, and addition of anti-Ia sera directly to cultures blocked the proliferative response at the stimulator (RCS) cell level. Lymph node cells from H-2(8) strains other than SJL/J, including A.SW and B10.S also gave proliferative responses to RCS cells, although lower in magnitude. A requirement on the part of responding cells for identity with RCS cells at the Ir region was indicated by the finding that A.TH but not A.TL lymph node cells responded to RCS. It is concluded that RCS cells stimulate Ir-region identical T cells (without evidence of presensitization) through a modification in the expression of Ia antigens on the surface of the tumor cells.

Sign in / Sign up

Export Citation Format

Share Document