scholarly journals Stage-specific and species-specific antigens of Plasmodium vivax and Plasmodium ovale defined by monoclonal antibodies.

1986 ◽  
Vol 54 (3) ◽  
pp. 609-612 ◽  
Author(s):  
P M Andrysiak ◽  
W E Collins ◽  
G H Campbell
Keyword(s):  
Monoclonal Antibodies ◽  
Plasmodium Vivax ◽  
Plasmodium Ovale ◽  
Specific Antigens ◽  
Species Specific

Monoclonal antibodies to isolate-, species-, and genus-specific components on the surface of zoospores and cysts of the fungus Phytophthora cinnamomi

1986 ◽  
Vol 64 (2) ◽  
pp. 311-321 ◽  
Author(s):  
A. R. Hardham ◽  
E. Suzaki ◽  
J. L. Perkin
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Lines ◽  
Fungal Pathogens ◽  
Phytophthora Cinnamomi ◽  
Pathogenic Fungus ◽  
Immunofluorescence Assay ◽  
Pythium Species ◽  
Specific Antigens ◽  
Species Specific ◽  
First Time

Monoclonal antibodies have been raised to components on the surface of glutaraldehyde-fixed zoospores and cysts of an isolate of the pathogenic fungus Phytophthora cinnamomi. Hybridoma supernatants were screened using an immunofluorescence assay, and of 35 cell lines producing antibodies that reacted with the P. cinnamomi cells, 10 have been selected and their specificities examined in detail. The monoclonal antibodies were found to possess a valuable spectrum of taxonomic specificities, and have revealed, for the first time, the presence of isolate-specific antigens on the surface of P. cinnamomi cells. The monoclonal antibodies were tested against six isolates of P. cinnamomi, six species of Phytophthora, and three species of Pythium. In addition to the isolate-specific monoclonal antibodies, species-specific and genus-specific markers which are unambiguous in tests conducted so far have been obtained. The monoclonal antibodies have also revealed the presence of spatially restricted antigens on the surface of the zoospores. Some of these segregated antigens are species-specific and others are more general, occurring in all Phytophthora and Pythium species examined. All of the monoclonal antibodies promise to be of great assistance in investigations of the biology and taxonomy of P. cinnamomi. The methods described should be readily applicable to studies of other fungal pathogens.

scholarly journals Monoclonal antibodies against species-specific antigens in the chick central nervous system: putative application as transplantation markers in the chick-quail chimera.

1989 ◽  
Vol 37 (2) ◽  
pp. 177-184 ◽  
Author(s):  
S Takagi ◽  
T Tsuji ◽  
M Kinutani ◽  
H Fujisawa
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
Cell Types ◽  
Nerve Fibers ◽  
Ependymal Cells ◽  
Neuronal Tissues ◽  
Specific Antigens ◽  
Cellular Markers ◽  
Species Specific ◽  
Large Neurons

With the recent progress in transplantation of neuronal tissues, cellular markers are needed to distinguish the grafted cells from the host. To generate monoclonal antibodies (MAb) recognizing species-specific antigens in the chick nervous system, we immunized mice with chick optic nerves and obtained 2 MAb which bind to chick but not to quail neural tissues. MAb-39B11 recognizes the cell surface antigen on the nerve fibers. MAb-37F5 recognizes the cytoplasmic components in several cell types, including ependymal cells and some large neurons. The utility of these MAb as markers for chick cells in the chick-quail chimeric brain and their advantages over conventional markers are discussed.

Reactivity of Monoclonal Antibodies to Species-Specific Antigens ofEntamoeba histolytica

1991 ◽  
Vol 38 (4) ◽  
pp. 329-334 ◽  
Author(s):  
HIROSHI TACHIBANA ◽  
SEIKI KOBAYASHI ◽  
KOUICHI NAGAKURA ◽  
YOSHIMASA KANEDA ◽  
TSUTOMU TAKEUCHI
Keyword(s):  
Monoclonal Antibodies ◽  
Ofentamoeba Histolytica ◽  
Specific Antigens ◽  
Species Specific

Monoclonal antibodies to cell surface components of zoospores and cysts of the fungusPythium aphanidermatum reveal species-specific antigens

1989 ◽  
Vol 13 (4) ◽  
pp. 348-355 ◽  
Author(s):  
M. Teresa Estrada-Garcia ◽  
Jonathan R. Green ◽  
Julia M. Booth ◽  
J. Geoffrey White ◽  
James A. Callow
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Specific Antigens ◽  
Species Specific

scholarly journals Trehalose-containing lipooligosaccharides. A new class of species-specific antigens from Mycobacterium.

1983 ◽  
Vol 258 (17) ◽  
pp. 10481-10487 ◽  
Author(s):  
S W Hunter ◽  
R C Murphy ◽  
K Clay ◽  
M B Goren ◽  
P J Brennan
Keyword(s):  
New Class ◽  
Specific Antigens ◽  
Species Specific

Sperm antigens and immunocontraception

1995 ◽  
Vol 7 (4) ◽  
pp. 825 ◽  
Author(s):  
LE Kerr
Keyword(s):  
Monoclonal Antibodies ◽  
World Population ◽  
Contraceptive Vaccine ◽  
The World ◽  
Specific Antigens ◽  
Population Crisis ◽  
Sperm Antigens

The development of novel forms of contraception is one way in which the world population crisis is being tackled. The concept of a contraceptive vaccine based on gamete-specific antigens is a particularly attractive approach. Much research has been carried out to identify sperm antigens which could be used as the immunogen. The most encouraging leads have come from groups using monoclonal antibodies to identify and characterize sperm antigens important for fertility (e.g. SP-10, PH-20 and PH-30). Identification of these molecules will also enable the development of specific tests for the diagnosis of immune infertility.

scholarly journals Importance of serological cross-reactivity amongToxoplasma gondii, Hammondiaspp.,Neosporaspp.,Sarcocystisspp. andBesnoitia besnoiti

Parasitology ◽  
2017 ◽  
Vol 144 (7) ◽  
pp. 851-868 ◽  
Author(s):  
LUÍS F. P. GONDIM ◽  
JOSÉ R. MINEO ◽  
GEREON SCHARES
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Cross Reactivity ◽  
Besnoitia Besnoiti ◽  
Cross Reactions ◽  
Epidemiological Surveys ◽  
Specific Proteins ◽  
Specific Antigens ◽  
Species Specific

SUMMARYToxoplasma gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andBesnoitia besnoitiare genetically related cyst-forming coccidia. Serology is frequently used for the identification ofT. gondii, Neosporaspp. andB. besnoiti-exposed individuals. Serologic cross-reactions occur in different tests among animals infected withT. gondiiandH. hammondi,as well as among animals infected byT. gondiiandN. caninum. Infections caused byN. caninumandN. hughesiare almost indistinguishable by serology.Neospora caninum, B. besnoitiandSarcocystisspp. infections in cattle show some degree of serologic cross-reactivity. Antibody cross-reactivity betweenNeosporaspp. andH. heydorni-infected animals is suspected, but not proven to occur. We review serologic cross-reactivity among animals and/or humans infected withT. gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andB. besnoiti. Emphasis is laid upon antigens and serological methods forN. caninumdiagnosis which were tested for cross-reactivity with related protozoa. Species-specific antigens, as well as stage-specific proteins have been identified in some of these parasites and have promising use for diagnosis and epidemiological surveys.

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