A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study.

Abstract Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro. Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Results: The results indicated that supernatants of these two lactobacilli had cytotoxic effects on Caco-2 cells at a concentration of 25% v/v and higher. Thus, the minimum concentrations (25% V/V) of supernatants were chosen for further experiments. LAS and LRS could significantly suppress the invasiveness of Caco-2 cells. Also, the expression of MMP12 was significantly increased in Caco-2 cells when treated with LAS, whereas LRS had no significant effect on the invasive capacity and the gene expression levels of MMP12. The expression of MMP-9 was statistically decreased in Caco2 cells treated with LAS and LRS (P<0.00001).Conclusion: In general, it was shown that LAS and LRS exert anti-cancer activity against the growth, invasion, and metastasis of Caco2 cells in-vitro. It seems that these two bacteria could be used as prophylactic and therapeutic agents for the prevention and treatment of colorectal cancer.

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Erratum to “Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: comparison of active and passive transport with the human colon carcinoma Caco-2 cell line”

Environmental Toxicology and Pharmacology ◽

10.1016/s1382-6689(02)00123-0 ◽

2003 ◽

Vol 13 (1) ◽

pp. 55 ◽

Cited By ~ 5

Author(s):

Carolien H.M Versantvoort ◽

Rob C.A Ondrewater ◽

Erwin Duizer ◽

Johannes J.M Van de Sandt ◽

Andries J Gilde ◽

...

Keyword(s):

Cell Line ◽

Colon Carcinoma ◽

In Vitro Model ◽

Intestinal Barrier ◽

Human Colon ◽

Paracellular Transport ◽

Passive Transport ◽

Human Colon Carcinoma ◽

Vitro Model

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In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line: Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry

Archives of Toxicology ◽

10.1007/bf02733673 ◽

1995 ◽

Vol 70 (2) ◽

pp. 124-126 ◽

Cited By ~ 5

Author(s):

Micha Rabau ◽

Abraham Nyska ◽

Dan Dayan

Keyword(s):

Carcinoma Cell ◽

Nucleolar Organizer ◽

Human Colon ◽

Nucleolar Organizer Regions ◽

Thymidine Uptake ◽

Human Colon Carcinoma Cell ◽

Human Colon Carcinoma ◽

Vitro Effect ◽

Ht 29

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The use of fractal analysis to describe in vitro drug effects: Effect of lovastatin on CaCo-2 cell differentiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100169493 ◽

1994 ◽

Vol 52 ◽

pp. 352-353

Author(s):

Beverly E. Maleeff ◽

Timothy K. Hart ◽

Anne M. Klinkner ◽

C. Robbie Waites ◽

Peter J. Bugelski ◽

...

Keyword(s):

Cell Differentiation ◽

Carcinoma Cell ◽

Drug Effects ◽

Human Colon ◽

Human Colon Carcinoma Cell ◽

Polycarbonate Membrane ◽

Cell Height ◽

Human Colon Carcinoma ◽

Inhibit Cell Growth

Inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoAR) have been shown to inhibit cell growth and to affect cell differentiation in a variety of in vitro systems. The human colon carcinoma cell line CaCo-2, when grown on permeable membranes, undergoes high order differentiation into a well-organized monolayer of columnar enterocytes. However, in the presence of the HMG CoAR inhibitor lovastatin, these cells fail to differentiate, lose contact inhibition and overgrow the culture support. Changes in cell differentiation can be described by measurements of mean cell height (MCH) or mean monolayer height (MMH), and are illustrated with representative micrographs. In this study, we have applied fractal geometry in addition to traditional morphometry to quantitatively describe the effect of lovastatin on CaCo-2 cell differentiation in vitro.CaCo-2 cells were plated at sub-confluency (∽ 60,000 cells/cm2) on collagen-coated Transwell™ permeable polycarbonate membrane supports (Costar Corp., Cambridge, MA) in culture dishes and allowed to attach for at least 24 hours.

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