Polymorphonuclear Neutrophils Are Necessary for the Recruitment of CD8+ T Cells in the Liver in a Pregnant Mouse Model of Chlamydophila abortus (Chlamydia psittaci Serotype 1) Infection

ABSTRACT The role of polymorphonuclear neutrophils (PMNs) in the development of the specific immune response against Chlamydophila abortus (Chlamydia psittaci serotype 1) infection was studied in a pregnant mouse model involving treatment with RB6-8C5 monoclonal antibody. PMN depletion significantly affected the immune response in the liver, in which the T-lymphocyte and F4/80+cell populations decreased, particularly the CD8+ T-cell population. A Th1-like response, characterized by high levels of gamma interferon without detectable levels of interleukin 4 (IL-4) in serum, was observed in both depleted and nondepleted mice, although an increased production of IL-10 was detected in the depleted group. Our results suggest that PMNs play a very important role in the recruitment of other leukocyte populations to the inflammatory foci but have little influence in the polarization of the immune specific response toward a Th1-like response.

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Limited role of polymorphonuclear neutrophils in a pregnant mouse model of secondary infection by Chlamydophila abortus (Chlamydia psittaci serotype 1)

Microbial Pathogenesis ◽

10.1006/mpat.2000.0396 ◽

2000 ◽

Vol 29 (6) ◽

pp. 319-327 ◽

Cited By ~ 7

Author(s):

Roberto Montes de Oca ◽

Antonio J Buendı́a ◽

Joaquı́n Sánchez ◽

Laura Del Rı́o ◽

Juan Seva ◽

...

Keyword(s):

Mouse Model ◽

Secondary Infection ◽

Pregnant Mouse ◽

Chlamydia Psittaci ◽

Polymorphonuclear Neutrophils ◽

Chlamydophila Abortus ◽

Limited Role ◽

Serotype 1

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Chlamydophila abortus (Chlamydia psittaci serotype 1) Clearance is Associated with the Early Recruitment of Neutrophils and CD8+T Cells in a Mouse Model

Journal of Comparative Pathology ◽

10.1053/jcpa.2000.0411 ◽

2000 ◽

Vol 123 (2-3) ◽

pp. 171-181 ◽

Cited By ~ 22

Author(s):

L. Del Rio ◽

A.J. Buendia ◽

J. Sánchez ◽

B. Garcés ◽

M.R. Caro ◽

...

Keyword(s):

T Cells ◽

Mouse Model ◽

Cd8 T Cells ◽

Chlamydia Psittaci ◽

Chlamydophila Abortus ◽

Serotype 1 ◽

Early Recruitment

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B-Cell-Deficient Mice Show an Exacerbated Inflammatory Response in a Model of Chlamydophila abortus Infection

Infection and Immunity ◽

10.1128/iai.70.12.6911-6918.2002 ◽

2002 ◽

Vol 70 (12) ◽

pp. 6911-6918 ◽

Cited By ~ 24

Author(s):

Antonio J. Buendía ◽

Laura Del Río ◽

Nieves Ortega ◽

Joaquín Sánchez ◽

María C. Gallego ◽

...

Keyword(s):

Immune Response ◽

B Cells ◽

Inflammatory Response ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Immunoglobulin M ◽

Chlamydia Psittaci ◽

Chlamydophila Abortus ◽

Serotype 1

ABSTRACT The resolution of Chlamydophila abortus (Chlamydia psittaci serotype 1) infection is dependent on gamma interferon and CD8+ T cells, and classically, B cells have been considered to play a minimal role in host defense. The role of B cells in the immune response was studied by using a model of infection in mice with genetically modified immunoglobulin M transmembrane domains (μMT). In the absence of B cells, infection with C. abortus leads to an acute severe fatal disease that involves a disseminated intravascular coagulation syndrome. μMT mice displayed an increased level of proinflammatory cytokines in serum, and an increased number of neutrophils was observed in the lesions. The possible deleterious role of neutrophils in the pathogenesis of disease in μMT mice was determined by depletion of the neutrophils with the monoclonal antibody RB6-8C5. This led to an enhancement of the bacterial burden and early mortality in both μMT and wild-type mice, while necrotic lesions remained. Analysis of the presence of immunoregulatory cytokines showed significantly lower levels of transforming growth factor β in the sera of μMT mice. However, mice lacking mature B cells were able to establish a specific immune response that protected them from a secondary challenge. Taken together, these data suggest an immunomodulatory role for B cells in the early events of C. abortus primary infection that can protect mice against an exaggerated inflammatory response.

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Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion

Infection and Immunity ◽

10.1128/iai.67.5.2110-2116.1999 ◽

1999 ◽

Vol 67 (5) ◽

pp. 2110-2116 ◽

Cited By ~ 36

Author(s):

Antonio J. Buendía ◽

Roberto Montes de Oca ◽

Jose A. Navarro ◽

Joaquín Sánchez ◽

Francisco Cuello ◽

...

Keyword(s):

Mononuclear Cells ◽

Neutrophil Infiltration ◽

Pregnant Mouse ◽

Chlamydia Psittaci ◽

Polymorphonuclear Neutrophils ◽

Histopathological Analysis ◽

Serotype 1 ◽

Intraperitoneal Infection ◽

Chlamydial Antigen

ABSTRACT To assess the role of polymorphonuclear neutrophils (PMNs) inChlamydia psittaci infection in a pregnant mouse model, pregnant and nonpregnant Swiss OF1 mice were depleted of PMNs by treatment with the RB6-8C5 monoclonal antibody before intraperitoneal infection with C. psittaci serotype 1. Nondepleted mice served as infection controls. Depleted mice aborted earlier and had a much higher mortality rate than nondepleted mice. Bacteriological analysis showed that the number of chlamydiae isolated from the spleens of depleted mice at 5 and 7 days postinfection was 100 times greater than that isolated from nondepleted mice. Histopathological analysis of the placentas of depleted mice showed widespread necrosis of the uteroplacental units, with weak immunoreaction to chlamydial antigen, while the placentas of nondepleted mice showed substantial neutrophil infiltration but no large areas of necrosis, with moderate to strong immunoreaction to chlamydial antigen. The livers of depleted mice showed numerous chlamydial inclusions in the hepatocytes, delayed microgranuloma formation, and in the pregnant animals extensive coagulative periportal necrosis. The livers of nondepleted mice displayed multiple small foci of PMNs and mononuclear cells with microgranuloma formation. Among this group of mice, the pregnant animals always had more hepatic damage than nonpregnant animals. Our results suggest that PMNs play an essential role in the response to C. psittaci primary infection, preventing the uncontrolled multiplication of chlamydiae in the liver and spleen.

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Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection

Veterinary Microbiology ◽

10.1016/j.vetmic.2010.01.025 ◽

2010 ◽

Vol 144 (3-4) ◽

pp. 392-398 ◽

Cited By ~ 3

Author(s):

Karen Kerr ◽

Nicholas Wheelhouse ◽

Morag Livingstone ◽

Ian E. Anderson ◽

Gary Entrican ◽

...

Keyword(s):

Mouse Model ◽

Inflammatory Cytokine ◽

Pregnant Mouse ◽

Chlamydophila Abortus ◽

Cytokine Responses

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Chlamydiose abortive des petits ruminants au Maroc : valeur épidémiologique d’un kit Elisar de Chlamydophila abortus (Chlamydia psittaci sérotype 1)

Revue d’élevage et de médecine vétérinaire des pays tropicaux ◽

10.19182/remvt.9773 ◽

2001 ◽

Vol 54 (3-4) ◽

pp. 201

Author(s):

H. Salih Alj Debbarh ◽

H. Hasnaoui ◽

A. Souriau ◽

A. Belhouari ◽

R. Saïle ◽

...

Keyword(s):

Chlamydia Psittaci ◽

Chlamydophila Abortus ◽

Test Elisa ◽

Serotype 1

Le test Elisa recombinant (Elisar) utilisant la protéine de 80 à 90 kDa spécifique de Chlamydophila abortus, développé à l’Inra de Nouzilly en France, a été évalué au Maroc. Trois cent sept sérums appartenant à cinq groupes d’ovins et de caprins, provenant d’élevages aux taux d’avortements élevés ou bien présentés pour contrôle vétérinaire, ont été testés. La comparaison des résultats obtenus avec le test de fixation du complément (Tfc) a montré une grande discordance entre les deux techniques. En effet, seulement 16 sérums ont été positifs avec Elisar contre 130 positifs avec Tfc. En particulier, parmi les 55 sérums provenant d’animaux avortés, 48 ont été positifs avec Tfc et 16 avec Elisar. Ceci soulève avec acuité la problématique du rôle de Chlamydophila pecorum dans les avortements des petits ruminants au Maroc. La recherche différentielle des pathologies à Chlamydophila abortus et Chlamydophila pecorum est nécessaire pour préciser l’origine des divergences observées entre Elisar et Tfc.

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