scholarly journals Immunoprotection of Recombinant Leptospiral Immunoglobulin-Like Protein A against Leptospira interrogans Serovar Pomona Infection

2006 ◽  
Vol 74 (3) ◽  
pp. 1745-1750 ◽  
Author(s):  
Raghavan U. M. Palaniappan ◽  
Sean P. McDonough ◽  
Thomas J. Divers ◽  
Chia-Sui Chen ◽  
Ming-Jeng Pan ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Protein A ◽  
Terminal Region ◽  
Leptospira Interrogans ◽  
Hamster Model ◽  
Amino Terminal ◽  
Leptospira Interrogans Serovar Pomona ◽  
Antibody Titers ◽  
Potential Vaccine ◽  
Phosphate Buffered Saline

ABSTRACTWe previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins LigA and LigB ofLeptospira interrogans. LigA and LigB are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. Here, we evaluate the potential of recombinant LigA (rLigA) as a vaccine candidate against infection byL. interrogansserovar Pomona in a hamster model. rLigA was truncated into conserved (rLigAcon) and variable (rLigAvar) regions and expressed inEscherichia colias a fusion protein with glutathione-S-transferase (rLigA). Golden Syrian hamsters were immunized at 3 and 6 weeks of age with rLigA (rLigAcon and rLigAvar) with aluminum hydroxide as an adjuvant. Hamsters given recombinant glutathione-S-transferase (rGST)-adjuvant and phosphate-buffered saline-adjuvant served as nonvaccinated controls. Three weeks after the last vaccination, all animals were challenged intraperitoneally with 108L. interrogansserovar Pomona bacteria (NVSL 1427-35-093002). All hamsters immunized with recombinant LigA survived after challenge and had no significant histopathological changes. In contrast, nonimmunized and rGST-immunized hamsters were subjected to lethal doses, and the hamsters that survived showed severe tubulointerstitial nephritis. All vaccinated animals showed a rise in antibody titers against rLigA. Results from this study indicate that rLigA is a potential vaccine candidate againstL. interrogansserovar Pomona infection.

scholarly journals Recognition of pneumococcal isolates by antisera raised against PspA fragments from different clades

2008 ◽  
Vol 57 (3) ◽  
pp. 273-278 ◽  
Author(s):  
Michelle Darrieux ◽  
Adriana T. Moreno ◽  
Daniela M. Ferreira ◽  
Fabiana C. Pimenta ◽  
Ana Lúcia S. S. de Andrade ◽  
...  
Keyword(s):  
Animal Models ◽  
Vaccine Candidate ◽  
Protein A ◽  
Surface Protein ◽  
Structural Diversity ◽  
Cross Reactivity ◽  
Terminal Region ◽  
Hybrid Protein ◽  
Pneumococcal Infections ◽  
Clade 1

Pneumococcal surface protein A (PspA) is an important vaccine candidate against pneumococcal infections, capable of inducing protection in different animal models. Based on its structural diversity, it has been suggested that a PspA-based vaccine should contain at least one fragment from each of the two major families (family 1, comprising clades 1 and 2, and family 2, comprising clades 3, 4 and 5) in order to elicit broad protection. This study analysed the recognition of a panel of 35 pneumococcal isolates bearing different PspAs by antisera raised against the N-terminal regions of PspA clades 1 to 5. The antiserum to PspA clade 4 was found to show the broadest cross-reactivity, being able to recognize pneumococcal strains containing PspAs of all clades in both families. The cross-reactivity of antibodies elicited against a PspA hybrid including the N-terminal region of clade 1 fused to a shorter and more divergent fragment (clade-defining region, or CDR) of clade 4 (PspA1–4) was also tested, and revealed a strong recognition of isolates containing clades 1, 4 and 5, and weaker reactions with clades 2 and 3. The analysis of serum reactivity against different PspA regions further revealed that the complete N-terminal region rather than just the CDR should be included in an anti-pneumococcal vaccine. A PspA-based vaccine is thus proposed to be composed of the whole N-terminal region of clades 1 and 4, which could also be expressed as a hybrid protein.

scholarly journals An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis

2009 ◽  
Vol 58 (12) ◽  
pp. 1632-1637 ◽  
Author(s):  
Adenizar D. Chagas-Junior ◽  
Alan J. A. McBride ◽  
Daniel A. Athanazio ◽  
Cláudio P. Figueira ◽  
Marco A. Medeiros ◽  
...  
Keyword(s):  
Preparation Method ◽  
Vaccine Candidate ◽  
Hamster Model ◽  
Blood Samples ◽  
Vaccine Candidates ◽  
End Point ◽  
Touch Preparation ◽  
Potential Vaccine ◽  
Kidney Liver ◽  
Sterilizing Immunity

In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (κ=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (κ=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.

scholarly journals Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt

2018 ◽  
Vol 6 (23) ◽  
Author(s):  
Luisa Zanolli Moreno ◽  
Fabiana Miraglia ◽  
Carolina Helena de Oliveira ◽  
Silvio Arruda Vasconcellos ◽  
Marcos Bryan Heinemann ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Carrier State ◽  
Draft Genome Sequence ◽  
Leptospira Interrogans ◽  
High Antibody ◽  
Content Type ◽  
Leptospira Interrogans Serovar Pomona ◽  
Antibody Titers ◽  
Apparently Healthy

ABSTRACT Leptospira interrogans serovar Pomona is one of the most important serovars associated with worldwide porcine leptospirosis, and its infection is characterized by high antibody titers and the establishment of a renal carrier state. Here, we present the draft genome sequence of Leptospira interrogans serovar Pomona strain GR5 isolated from apparently healthy gilt in Brazil.

scholarly journals Pneumococcal Surface Protein A: A Promising Candidate for the Next Generation of Pneumococcal Vaccines

2022 ◽  
Vol 67 (4) ◽  
pp. 289-298
Author(s):  
Saad Alghamdi ◽  
Muhammad Umar Khayam Sahibzada ◽  
Nashwa T. Shesha ◽  
Akhmed Aslam ◽  
Ahmed Kabrah ◽  
...  
Keyword(s):  
Immune Responses ◽  
Pneumococcal Disease ◽  
Vaccine Candidate ◽  
Protein A ◽  
Surface Protein ◽  
Pneumococcal Infections ◽  
Promising Candidate ◽  
Host Immune Responses ◽  
Pneumococcal Surface Protein A ◽  
Potential Vaccine

Streptococcus pneumoniae is the bacterium that causes pneumococcal disease which often results in pneumonia, meningitis, otitis media, septicemia and sinusitis. Pneumonia, particularly, is a significant cause of worldwide morbidity and a global health burden as well. Treatment often relies on antimicrobials, to which the pathogen is frequently mutating and rendering infective. Consequently, vaccination is the most effective approach in dealing with pneumococcal antimicrobial resistance (AMR). Unfortunately, the current pneumococcal polysaccharide and conjugate vaccines have a narrow serotype coverage. Therefore, the current need for vaccines with a broader serotype coverage cannot be overstated. Pneumococcal Surface Protein A and C are potential vaccine candidate antigens present in over 90% of the strains from clinical isolates as well as laboratory non-encapsulated strains. Pneumococcal Surface Protein A is an active virulent factor that pneumococci use to evade complement-mediated host immune responses and has been shown to elicit immune responses against pneumococcal infections. This review explores the potential utilization of Pneumococcal Surface Protein A to immunize against S. pneumoniae.

scholarly journals Recombinant Neisseria surface protein A is a potential vaccine candidate against Neisseria meningitides serogroup B

2014 ◽  
Vol 10 (3) ◽  
pp. 1619-1625 ◽  
Author(s):  
SHANGYUN YING ◽  
JUN HE ◽  
MINJUN YU ◽  
YUKUAI ZHANG ◽  
SUHONG DENG ◽  
...  
Keyword(s):  
Vaccine Candidate ◽  
Protein A ◽  
Surface Protein ◽  
Potential Vaccine Candidate ◽  
Neisseria Meningitides ◽  
Potential Vaccine

Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis

Parasitology ◽  
2020 ◽  
Vol 147 (12) ◽  
pp. 1330-1337
Author(s):  
Marianna Nascimento Manhani ◽  
Cristiane Queixa Tilelli ◽  
Vanessa da Silva Ribeiro ◽  
Luiz Ricardo Goulart ◽  
Julia Maria Costa-Cruz
Keyword(s):  
Vaccine Candidate ◽  
Taenia Solium ◽  
Public Health Problem ◽  
Positive Control ◽  
Negative Control ◽  
Serum Samples ◽  
Vaccine Candidates ◽  
Potential Vaccine ◽  
Phosphate Buffered Saline ◽  
Parasite Challenge

AbstractHuman cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate in experimental murine cysticercosis. For this, BALB/c mice were immunized with mCc48 (G1; n = 40), sCc48 (G2; n = 40) and phosphate-buffered saline (PBS) (G3; n = 40, positive control) and challenged with Taenia crassiceps metacestodes. Another PBS group without parasite challenge was used as a negative control (G4; n = 40). Mice were sacrificed 15, 30, 45 and 60 days post-infection for cysticerci and serum collection. Immunization efficacy was determined by cysticerci counting. Serum samples were tested by ELISA to verify antibody (IgM, IgG, IgA and IgE) and cytokine (IFNγ and IL-4) levels. The sCc48 achieved the highest rates of protection and efficacy (90 and 98%, respectively). The group immunized with mCc48 presented the highest reactivity for IgM, IgG and IgE. All groups presented IL-4, but IFNγ was quite variable among groups. The protection induced by sCc48 synthetic peptide supports further studies of this mimotope as a potential vaccine candidate against cysticercosis.

scholarly journals Immunogenicity in a Mouse Model of a Conjugate Vaccine Made with a Synthetic Single Repeating Unit of Type 14 Pneumococcal Polysaccharide Coupled to CRM197

2002 ◽  
Vol 70 (9) ◽  
pp. 5107-5114 ◽  
Author(s):  
Fatme Mawas ◽  
Jutta Niggemann ◽  
Christopher Jones ◽  
Michael J. Corbel ◽  
Johannis P. Kamerling ◽  
...  
Keyword(s):  
Mouse Model ◽  
Antibody Response ◽  
Adipic Acid ◽  
Capsular Polysaccharide ◽  
Vaccine Candidate ◽  
Repeat Unit ◽  
Enzyme Linked Immunosorbent Assay ◽  
Galactose Residue ◽  
Antibody Titers ◽  
Potential Vaccine

ABSTRACT Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide fragment obtained by Pn14PS depolymerization were good inhibitors. CRM197 conjugates of the tetrasaccharide and an octasaccharide mimic were prepared by using either adipic acid diester or diethyl squarate linkers. The conjugate with the tetrasaccharide chains induced anti-Pn14PS antibodies when injected subcutaneously into mice, as determined by an enzyme-linked immunosorbent assay, and antibody titers increased with oligosaccharide loading. The adipic acid-linked tetrasaccharide conjugates elicited higher antibody titers than those prepared with a squarate spacer. The lower anti-Pn14PS antibody response of the octasaccharide mimic conjugate indicates the importance of the backbone galactose residue for an appropriate antibody response. The OS-CRM197 conjugate prepared from a single repeat unit of the Pn14PS is a potential vaccine candidate.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

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