scholarly journals Transcriptional Regulation of Central Carbon and Energy Metabolism in Bacteria by Redox-Responsive Repressor Rex

2011 ◽  
Vol 194 (5) ◽  
pp. 1145-1157 ◽  
Author(s):  
D. A. Ravcheev ◽  
X. Li ◽  
H. Latif ◽  
K. Zengler ◽  
S. A. Leyn ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Energy Metabolism ◽  
Redox Responsive ◽  
Central Carbon

scholarly journals HexR Controls Glucose-Responsive Genes and Central Carbon Metabolism in Neisseria meningitidis

2015 ◽  
Vol 198 (4) ◽  
pp. 644-654 ◽  
Author(s):  
Ana Antunes ◽  
Giacomo Golfieri ◽  
Francesca Ferlicca ◽  
Marzia M. Giuliani ◽  
Vincenzo Scarlato ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Neisseria Meningitidis ◽  
Microarray Analysis ◽  
Carbon Metabolism ◽  
Tricarboxylic Acid Cycle ◽  
Transcriptional Regulator ◽  
Central Carbon Metabolism ◽  
Binding Motif ◽  
Content Type ◽  
Central Carbon

ABSTRACTNeisseria meningitidis, an exclusively human pathogen and the leading cause of bacterial meningitis, must adapt to different host niches during human infection.N. meningitidiscan utilize a restricted range of carbon sources, including lactate, glucose, and pyruvate, whose concentrations vary in host niches. Microarray analysis ofN. meningitidisgrown in a chemically defined medium in the presence or absence of glucose allowed us to identify genes regulated by carbon source availability. Most such genes are implicated in energy metabolism and transport, and some are implicated in virulence. In particular, genes involved in glucose catabolism were upregulated, whereas genes involved in the tricarboxylic acid cycle were downregulated. Several genes encoding surface-exposed proteins, including the MafA adhesins andNeisseriasurface protein A, were upregulated in the presence of glucose. Our microarray analysis led to the identification of a glucose-responsivehexR-like transcriptional regulator that controls genes of the central carbon metabolism ofN. meningitidisin response to glucose. We characterized the HexR regulon and showed that thehexRgene is accountable for some of the glucose-responsive regulation;in vitroassays with the purified protein showed that HexR binds to the promoters of the central metabolic operons of the bacterium. Based on DNA sequence alignment of the target sites, we propose a 17-bp pseudopalindromic consensus HexR binding motif. Furthermore,N. meningitidisstrains lackinghexRexpression were deficient in establishing successful bacteremia in an infant rat model of infection, indicating the importance of this regulator for the survival of this pathogenin vivo.IMPORTANCENeisseria meningitidisgrows on a limited range of nutrients during infection. We analyzed the gene expression ofN. meningitidisin response to glucose, the main energy source available in human blood, and we found that glucose regulates many genes implicated in energy metabolism and nutrient transport, as well as some implicated in virulence. We identified and characterized a transcriptional regulator (HexR) that controls metabolic genes ofN. meningitidisin response to glucose. We generated a mutant lacking HexR and found that the mutant was impaired in causing systemic infection in animal models. SinceN. meningitidislacks known bacterial regulators of energy metabolism, our findings suggest that HexR plays a major role in its biology by regulating metabolism in response to environmental signals.

scholarly journals KSHV Reprogramming of Host Energy Metabolism for Pathogenesis

2021 ◽  
Vol 11 ◽  
Author(s):  
Xiaoqing Liu ◽  
Caixia Zhu ◽  
Yuyan Wang ◽  
Fang Wei ◽  
Qiliang Cai
Keyword(s):  
Energy Metabolism ◽  
Metabolic Pathways ◽  
B Lymphocyte ◽  
Aerobic Glycolysis ◽  
Cell Metabolism ◽  
Acid Synthesis ◽  
Central Carbon Metabolism ◽  
Central Carbon ◽  
Cell Energy ◽  
Infected Cells

Reprogramming of energy metabolism is a key for cancer development. Kaposi’s sarcoma-associated herpesvirus (KSHV), a human oncogenic herpesvirus, is tightly associated with several human malignancies by infecting B-lymphocyte or endothelial cells. Cancer cell energy metabolism is mainly dominated by three pathways of central carbon metabolism, including aerobic glycolysis, glutaminolysis, and fatty acid synthesis. Increasing evidence has shown that KSHV infection can alter central carbon metabolic pathways to produce biomass for viral replication, as well as the survival and proliferation of infected cells. In this review, we summarize recent studies exploring how KSHV manipulates host cell metabolism to promote viral pathogenesis, which provides the potential therapeutic targets and strategies for KSHV-associated cancers.

scholarly journals Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP

2016 ◽  
Author(s):  
Donghyuk Kim ◽  
Sang Woo Seo ◽  
Hojung Nam ◽  
Gabriela I. Guzman ◽  
Ye Gao ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

scholarly journals Systems assessment of transcriptional regulation on central carbon metabolism by Cra and CRP

2018 ◽  
Vol 46 (6) ◽  
pp. 2901-2917 ◽  
Author(s):  
Donghyuk Kim ◽  
Sang Woo Seo ◽  
Ye Gao ◽  
Hojung Nam ◽  
Gabriela I Guzman ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

scholarly journals CceR and AkgR Regulate Central Carbon and Energy Metabolism in Alphaproteobacteria

mBio ◽  
2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Saheed Imam ◽  
Daniel R. Noguera ◽  
Timothy J. Donohue
Keyword(s):  
Energy Metabolism ◽  
Central Carbon

scholarly journals Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress

2002 ◽  
Vol 68 (2) ◽  
pp. 881-892 ◽  
Author(s):  
Dorothea K. Thompson ◽  
Alexander S. Beliaev ◽  
Carol S. Giometti ◽  
Sandra L. Tollaksen ◽  
Tripti Khare ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcriptional Regulation ◽  
Energy Metabolism ◽  
Type Strain ◽  
Wild Type Strain ◽  
Shewanella Oneidensis ◽  
Anaerobic Growth ◽  
Ferric Uptake Regulator ◽  
Wild Type ◽  
And Oxidative Stress

ABSTRACT The iron-directed, coordinate regulation of genes depends on the fur (ferric uptake regulator) gene product, which acts as an iron-responsive, transcriptional repressor protein. To investigate the biological function of a fur homolog in the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1, a fur knockout strain (FUR1) was generated by suicide plasmid integration into this gene and characterized using phenotype assays, DNA microarrays containing 691 arrayed genes, and two-dimensional polyacrylamide gel electrophoresis. Physiological studies indicated that FUR1 was similar to the wild-type strain when they were compared for anaerobic growth and reduction of various electron acceptors. Transcription profiling, however, revealed that genes with predicted functions in electron transport, energy metabolism, transcriptional regulation, and oxidative stress protection were either repressed (ccoNQ, etrA, cytochrome b and c maturation-encoding genes, qor, yiaY, sodB, rpoH, phoB, and chvI) or induced (yggW, pdhC, prpC, aceE, fdhD, and ppc) in the fur mutant. Disruption of fur also resulted in derepression of genes (hxuC, alcC, fhuA, hemR, irgA, and ompW) putatively involved in iron uptake. This agreed with the finding that the fur mutant produced threefold-higher levels of siderophore than the wild-type strain under conditions of sufficient iron. Analysis of a subset of the FUR1 proteome (i.e., primarily soluble cytoplasmic and periplasmic proteins) indicated that 11 major protein species reproducibly showed significant (P < 0.05) differences in abundance relative to the wild type. Protein identification using mass spectrometry indicated that the expression of two of these proteins (SodB and AlcC) correlated with the microarray data. These results suggest a possible regulatory role of S. oneidensis MR-1 Fur in energy metabolism that extends the traditional model of Fur as a negative regulator of iron acquisition systems.

scholarly journals The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei

2021 ◽  
Author(s):  
Anish Das ◽  
Tong Liu ◽  
Hong Li ◽  
Seema Husain
Keyword(s):  
Energy Metabolism ◽  
Rna Binding ◽  
Binding Protein ◽  
Critical Role ◽  
Rna Binding Protein ◽  
Mrna Translation ◽  
Cellular Energy ◽  
Target Mrna ◽  
Cellular Energy Metabolism ◽  
Central Carbon

AbstractRNA-binding proteins are key players in coordinated post-transcriptional regulation of functionally related genes, defined as RNA regulons. RNA regulons play particularly critical roles in parasitic trypanosomes, which exhibit unregulated co-transcription of long arrays of unrelated genes. In this report, we present a systematic analysis of an essential RNA-binding protein, RBP42, in the mammalian-infective slender bloodstream form of African trypanosome, and we show that RBP42 is a key regulator of parasite’s central carbon and energy metabolism. Using individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) to identify genome-wide RBP42-RNA interactions, we show that RBP42 preferentially binds within the coding region of mRNAs encoding core metabolic enzymes. Using global quantitative transcriptomic and proteomic analyses, we also show that loss of RBP42 reduces the abundance of target mRNA-encoded proteins, but not target mRNA, suggesting a plausible role of RBP42 as a positive regulator of target mRNA translation. Analysis reveals significant changes in central carbon metabolic intermediates following loss of RBP42, further supporting its critical role in cellular energy metabolism.

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