Horizontal Transfer of CS1 Pilin Genes of Enterotoxigenic Escherichia coli

ABSTRACT CS1 is one of a limited number of serologically distinct pili found in enterotoxigenic Escherichia coli (ETEC) strains associated with disease in people. The genes for the CS1 pilus are on a large plasmid, pCoo. We show that pCoo is not self-transmissible, although our sequence determination for part of pCoo shows regions almost identical to those in the conjugative drug resistance plasmid R64. When we introduced R64 into a strain containing pCoo, we found that pCoo was transferred to a recipient strain in mating. Most of the transconjugant pCoo plasmids result from recombination with R64, leading to acquisition of functional copies of all of the R64 transfer genes. Temporary coresidence of the drug resistance plasmid R64 with pCoo leads to a permanent change in pCoo so that it is now self-transmissible. We conclude that when R64-like plasmids are transmitted to an ETEC strain containing pCoo, their recombination may allow for spread of the pCoo plasmid to other enteric bacteria.

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Multiplex Polymerase Chain Reaction Assay for Identification of Enterotoxigenic Escherichia Coli Strains

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870101300405 ◽

2001 ◽

Vol 13 (4) ◽

pp. 308-311 ◽

Cited By ~ 22

Author(s):

Jacek Osek

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Direct Determination ◽

Enteric Bacteria ◽

Enterotoxigenic Escherichia Coli ◽

Chain Reaction ◽

Gram Negative ◽

E Coli ◽

Polymerase Chain

A multiplex polymerase chain reaction (PCR) system was developed for identification of enterotoxigenic Escherichia coli (ETEC) strains and to differentiate them from other gram negative enteric bacteria. This test simultaneously amplifies heat-labile (LTI) and heat-stable (STI and STII) toxin sequences and the E. coli-specific universal stress protein ( uspA). The specificity of the method was validated by single PCR tests performed with the reference E. coli and non- E. coli strains and with bacteria isolated from pig feces. The multiplex PCR allowed the rapid and specific identification of enterotoxin-positive E. coli and may be used as a method for direct determination of ETEC and to differentiate them from other E. coli and gram-negative enteric isolates.

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Escherichia coli gene transfer to unrelated bacteria by a histidine operon — RP1 drug resistance plasmid complex

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(74)80217-2 ◽

1974 ◽

Vol 59 (1) ◽

pp. 377-385 ◽

Cited By ~ 15

Author(s):

Ronald H. Olsen ◽

Carlos Gonzalez

Keyword(s):

Escherichia Coli ◽

Drug Resistance ◽

Gene Transfer ◽

Resistance Plasmid ◽

Plasmid Complex

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Observations on production of hemolysin, heat-labile enterotoxin and antimicrobial drug resistance among enterotoxigenic Escherichia coli from pigs

Comparative Immunology Microbiology and Infectious Diseases ◽

10.1016/0147-9571(92)90001-8 ◽

1992 ◽

Vol 15 (4) ◽

pp. 229-234 ◽

Cited By ~ 1

Author(s):

H. Hariharan ◽

S. Heaney ◽

J. Bryenton ◽

J. Daley

Keyword(s):

Escherichia Coli ◽

Drug Resistance ◽

Antimicrobial Drug ◽

Enterotoxigenic Escherichia Coli ◽

Antimicrobial Drug Resistance ◽

Heat Labile

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Etiology of acute diarrhea in children in Shanghai, 2015-2018

10.21203/rs.2.18493/v1 ◽

2019 ◽

Author(s):

Hailing Chang ◽

Jiayin Guo ◽

Zhongqiu Wei ◽

Zheng Huang ◽

Chuning Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogens ◽

Acute Diarrhea ◽

Enteric Bacteria ◽

Enterohemorrhagic Escherichia Coli ◽

Enteric Pathogens ◽

Enterotoxigenic Escherichia Coli ◽

Salmonella Spp ◽

Shigella Spp ◽

Fresh Stool

Abstract Background Diarrhea is still a major cause of childhood morbidity and mortality worldwide. This hospital-based study aimed to monitor the consecutive epidemiological trend of etiology in children with acute diarrhea in Shanghai.Methods Outpatient children with diarrhea were prospectively enrolled within 7 days after onset of diarrheal symptoms during 2015-2018. Fresh stool samples were collected for testing enteropathogens. Enteric bacteria were identified and typed through culture and serotyping. Enteric viruses were identified through real-time PCR assay. hadResults Enteric pathogens were identified in 1572 (58.4%) of the 2692 enrolled children with acute diarrhea. Viruses were more frequently detected than bacteria (41.3% versus 25.0%), and co-infection with 2 or more pathogens was found in 13% of outpatients. Nontyphoidal Salmonella spp . (NTS) was the most common bacteria with 10.3% of isolate rate, followed by enteropathogenic Escherichia coli (EPEC) (6.5%), enteroaggregative Escherichia coli (EAEC) (6.2%), Campylobacter spp . (3.6%), enterotoxigenic Escherichia coli (ETEC) (1.1%), Shigella spp . (0.2%), and enterohemorrhagic Escherichia coli (EHEC) (0.1%). Rotavirus was the most common virus with 16.0% of detection rate, followed by norovirus (15.5%), adenovirus (7.2%), sapovirus (3.0%) and astrovirus (2.7%).Conclusions Infectious diarrhea remains the major cause of diarrhea in children in Shanghai. Rotavirus, norovirus and NTS were the major enteric pathogens responsible for diarrhea in Shanghainese children. Improving uptake of rotavirus vaccine and strengthening prevention of foodborne pathogens will be helpful to reduce the burden of diarrheal diseases in children in Shanghai.

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A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains

Journal of Bacteriology ◽

10.1128/jb.00349-07 ◽

2007 ◽

Vol 189 (16) ◽

pp. 6074-6079 ◽

Cited By ~ 10

Author(s):

Adaobi I. Nwaneshiudu ◽

Tania Mucci ◽

Derek J. Pickard ◽

Iruka N. Okeke

Keyword(s):

Escherichia Coli ◽

Subtractive Hybridization ◽

Selective Advantage ◽

Virulence Plasmid ◽

Conjugative Transfer ◽

Transfer System ◽

Large Plasmid ◽

Enteropathogenic Escherichia Coli ◽

Epec Strain ◽

Resistance Plasmid

ABSTRACT A novel and functional conjugative transfer system identified in O119:H2 enteropathogenic Escherichia coli (EPEC) strain MB80 by subtractive hybridization is encoded on a large multidrug resistance plasmid, distinct from the well-described EPEC adherence factor (EAF) plasmid. Variants of the MB80 conjugative resistance plasmid were identified in other EPEC strains, including the prototypical O111:NM strain B171, from which the EAF plasmid has been sequenced. This separate large plasmid and the selective advantage that it confers in the antibiotic era have been overlooked because it comigrates with the virulence plasmid on conventional gels.

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