scholarly journals Enzyme-linked immunosorbent assay for detection of antibodies against Streptococcus pneumoniae capsular polysaccharides.

1980 ◽  
Vol 11 (2) ◽  
pp. 198-199 ◽  
Author(s):  
H Russell ◽  
L R Edwards ◽  
E W Wortham ◽  
R R Facklam
Keyword(s):  
Streptococcus Pneumoniae ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capsular Polysaccharides

scholarly journals Evaluation of the Specificity of Pneumococcal Polysaccharide Enzyme-Linked Immunosorbent Assay and the Effect of Serum Adsorption Based on Standard Pneumococcal Serogroup- or Serotype-Specific Rabbit Antisera

2009 ◽  
Vol 16 (9) ◽  
pp. 1279-1284 ◽  
Author(s):  
Hans-Christian Slotved ◽  
Christina Guttmann ◽  
Charlotte Demuth Pedersen ◽  
Jasper Neergaard Jacobsen ◽  
Karen Angeliki Krogfelt
Keyword(s):  
Cell Wall ◽  
Streptococcus Pneumoniae ◽  
Human Serum ◽  
Immunosorbent Assay ◽  
Capsular Polysaccharide ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capsular Polysaccharides ◽  
Who Guidelines ◽  
Specific Antibodies ◽  
Specific Rabbit

ABSTRACT Worldwide, Streptococcus pneumoniae (pneumococcus) is a major cause of morbidity and mortality, especially in infants and elderly people. Pneumococcal capsular polysaccharides are well characterized, and more than 90 different serotypes have been identified. Serotype-specific antibodies against the capsular polysaccharide are produced during infection. Detection of antibodies against pneumococci by enzyme-linked immunosorbent assay (ELISA) is performed according to WHO guidelines, using antigens provided by ATCC. However, testing the ELISA for specificity is challenging due to the difficulty in obtaining human naïve serum with pneumococcal antibodies as well as human serum with antibodies against a single serotype. The application of well-defined serotype-specific sera produced in animals to evaluate the specificity of the ATCC antigens and the effect of adsorption with cell wall and 22F polysaccharides has not been performed before, to our knowledge. In this study, the specificity of ATCC antigens (serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F) was tested by using commercial serotype-, serogroup-, and pool-specific pneumococcal rabbit antisera.

scholarly journals Identification of a Secreted Cholesterol-Dependent Cytolysin (Mitilysin) from Streptococcus mitis

2006 ◽  
Vol 189 (2) ◽  
pp. 627-632 ◽  
Author(s):  
Johanna Jefferies ◽  
Leena Nieminen ◽  
Lea-Ann Kirkham ◽  
Calum Johnston ◽  
Andrew Smith ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Streptococcus Pneumoniae ◽  
Amino Acid ◽  
Immunosorbent Assay ◽  
Pneumococcal Disease ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Substitutions ◽  
Streptococcus Mitis ◽  
Hemolytic Toxin

ABSTRACT We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae, this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

scholarly journals Serodiagnosis of Streptococcus pneumoniae infection in guineapigs by an enzyme-linked immunosorbent assay

1988 ◽  
Vol 22 (4) ◽  
pp. 304-308 ◽  
Author(s):  
J. Matsubara ◽  
T. Kamiyama ◽  
T. Miyoshi ◽  
H. Ueda ◽  
M. Saito ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Field Study ◽  
Immunosorbent Assay ◽  
Simple Procedure ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Antibody ◽  
Long Period ◽  
Antibody Titres

Guineapig antibodies to Streptococcus pneumoniae (SPN) serotype 19F were detected by an enzyme-linked immunosorbent assay using a simple procedure. In experimentally infected hosts, antibody was detectable as early as 2 to 3 weeks after infection, and high titres were maintained for a long period. Antibodies higher than 1 : 64 were regarded as specific. In a field study, high antibody titres were shown in SPN enzootic colonies in contrast to negative or low antibody titres in a majority of the animals from non-enzootic and SPF colonies.

scholarly journals Assignment of Immunoglobulin G1 and G2 Concentrations to Pneumococcal Capsular Polysaccharides 3, 6B, 14, 19F, and 23F in Pneumococcal Reference Serum 89-SF

1998 ◽  
Vol 5 (4) ◽  
pp. 561-566 ◽  
Author(s):  
Anu Soininen ◽  
Ilkka Seppälä ◽  
Tomi Wuorimaa ◽  
Helena Käyhty
Keyword(s):  
Immunosorbent Assay ◽  
Capsular Polysaccharide ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capsular Polysaccharides ◽  
Serum Pool ◽  
Reference Serum

ABSTRACT We describe standardization of an enzyme-linked immunosorbent assay (ELISA) for measuring immunoglobulin G1 (IgG1) and IgG2 concentrations of antibodies to pneumococcal capsular polysaccharide (Pnc PS). The ELISA uses a human pneumococcal reference serum pool, lot 89-SF, as a reference. IgG1 and IgG2 concentrations were assigned to antibodies to Pnc PS serotypes 3, 6B, 14, 19F, and 23F in 89-SF by ELISA using affinity-purified monoisotypic IgG1 and IgG2 preparations. The sum of IgG1 and IgG2 concentrations in 89-SF agrees well with the previously assigned IgG concentrations. The IgG1 and IgG2 values in 89-SF were used to measure antibodies to Pnc PS 6B, 14, and 23F in adult pre- and postimmunization sera and the sum of IgG1 and IgG2 concentrations correlated well with the IgG values. Furthermore, the IgG2/IgG1 ratio did not affect the detection of IgG1, the isotype usually represented by a lower concentration.

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