scholarly journals Methicillin-Resistant Staphylococcus aureus Clonal Types in the Czech Republic

1999 ◽  
Vol 37 (9) ◽  
pp. 2798-2803 ◽  
Author(s):  
O. Melter ◽  
I. Santos Sanches ◽  
J. Schindler ◽  
M. Aires de Sousa ◽  
R. Mato ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Pfge Pattern ◽  
Type I ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Almost All ◽  
Genotypic Characteristics

Molecular surveillance studies have documented the extensive spread of methicillin-resistant Staphylococcus aureus (MRSA) clones. Studies carried out by Centro de Epidemiologia Molecular-Network for Tracking Gram-Positive Pathogenic Bacteria (CEM/NET) led to the identification of two international multidrug-resistant strains, which were designated as the Iberian and Brazilian MRSA clones and which were defined by multiple genomic typing methods; these included ClaI restriction digests hybridized with mecA- and Tn554-specific DNA probes and pulsed-field gel electrophoresis (PFGE). The genotypic characteristics of these clones are distinct: the Iberian clone is defined asmecA type I, Tn554 type E (or its variants), and PFGE pattern A (I:E:A), whereas the Brazilian clone is defined asmecA type XI (or its variants), Tn554 type B, and PFGE pattern B (XI:B:B). In this study, we characterized 59 single-patient isolates of MRSA collected during 1996 and 1997 at seven hospitals located in Prague and five other cities in the Czech Republic by using the methodologies mentioned above and by using ribotyping ofEcoRI and HindIII digests hybridized with a 16S-23S DNA probe. The Brazilian MRSA clone (XI:B:B) was the major clone (80%) spread in two hospitals located in Prague and one located in Brno; the Iberian MRSA clone (I:E:A or its variant I:DD:A), although less representative (12%), was detected in two hospitals, one in Prague and the other in Plzen. Almost all the strains belonging to clone XI:B:B (45 of 47) corresponded to a unique ribotype, E1H1, whereas most strains of the I:E:A and I:DD:A clonal types (6 of 7) corresponded to ribotype E2H2.

scholarly journals Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 395
Author(s):  
Katarina Pomorska ◽  
Vladislav Jakubu ◽  
Lucia Malisova ◽  
Marta Fridrichova ◽  
Martin Musilek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bloodstream Infections ◽  
Spa Typing ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Spa Types ◽  
Repeat Pattern

Staphylococcus aureus is one of the major causes of bloodstream infections. The aim of our study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from blood of patients hospitalized in the Czech Republic between 2016 and 2018. All MRSA strains were tested for antibiotic susceptibility, analyzed by spa typing and clustered using a Based Upon Repeat Pattern (BURP) algorithm. The representative isolates of the four most common spa types and representative isolates of all spa clonal complexes were further typed by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. The majority of MRSA strains were resistant to ciprofloxacin (94%), erythromycin (95.5%) and clindamycin (95.6%). Among the 618 strains analyzed, 52 different spa types were detected. BURP analysis divided them into six different clusters. The most common spa types were t003, t586, t014 and t002, all belonging to the CC5 (clonal complex). CC5 was the most abundant MLST CC of our study, comprising of 91.7% (n = 565) of spa-typeable isolates. Other CCs present in our study were CC398, CC22, CC8, CC45 and CC97. To our knowledge, this is the biggest nationwide study aimed at typing MRSA blood isolates from the Czech Republic.

scholarly journals Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Katerina Neradova ◽  
Vladislav Jakubu ◽  
Katarina Pomorska ◽  
Helena Zemlickova
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Nasal Carriage ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Mrsa Colonization ◽  
Animal Strains

Abstract Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

Carriage of methicillin-resistantStaphylococcus aureusin veterinary personnel

2009 ◽  
Vol 137 (9) ◽  
pp. 1233-1236 ◽  
Author(s):  
H. ŽEMLIČKOVÁ ◽  
M. FRIDRICHOVÁ ◽  
K. TYLLOVÁ ◽  
V. JAKUBŮ ◽  
I. MACHOVÁ
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Carriage Rate ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Genetic Lineages ◽  
Type Iv ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

SUMMARYA survey of 280 attendees at a veterinary meeting in the Czech Republic in 2008 revealed a carriage rate of 0·7% for methicillin-resistantStaphylococcus aureus(MRSA). The two strains isolated were of distinct genetic lineages, carried type IV SCCmecdeterminants and were negative for Panton–Valentine leukocidin genes. The MRSA positivity rates for veterinarians in the Czech Republic is considerably lower than reported elsewhere.

scholarly journals Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

2019 ◽  
Author(s):  
Katerina Neradova ◽  
Vladislav Jakubu ◽  
Katarina Pomorska ◽  
Helena Zemlickova
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nasal Carriage ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Mrsa Colonization

Abstract Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n=5), ST398-t2330-IV (n=1), ST398-t034-V (n=1), ST225-t003-II (n=1) and ST4894-t011-IV (n=1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

scholarly journals Methicillin-resistant Staphylococcus aureus in veterinary professionals in 2017 in the Czech Republic

2019 ◽  
Author(s):  
Katerina Neradova ◽  
Vladislav Jakubu ◽  
Katarina Pomorska ◽  
Helena Zemlickova
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Nasal Carriage ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Mrsa Strains ◽  
Healthcare Associated ◽  
Mrsa Colonization

Abstract Background Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. Results Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n=5), ST398-t2330-IV (n=1), ST398-t034-V (n=1), ST225-t003-II (n=1) and ST4894-t011-IV (n=1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex CC11. Conclusion According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

scholarly journals Occurrence and characteristic of methicillin-resistant Staphylococcus aureus on pig farms in the Czech Republic

2012 ◽  
Vol 81 (3) ◽  
pp. 219-223 ◽  
Author(s):  
Jan Bardoň ◽  
Milan Kolář ◽  
Renata Karpíšková ◽  
Helena Žemličková ◽  
Marta Fridrichová ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Nationwide Survey ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Pig Farms ◽  
Epidemiological Risk ◽  
Mrsa Strains ◽  
Pooled Samples ◽  
Single Sequence

In recent years, a negative trend of increasing prevalence of methicillin-resistantStaphylococcus aureus(MRSA) strains has been noted worldwide. In addition to their prevalence in humans, MRSA strains have also been detected in animals, both pets and food-producing animals, especially in pigs. At the same time, transmission of MRSA strains from animals to humans has been documented. This study brings results from the first nationwide survey on the prevalence of MRSA in herds of breeding pigs, carried out throughout the Czech Republic in accordance with the Commission Decision 2008/55/EC, including brief characteristics of isolates. In 2008, a total of 283 pooled samples (dust swabs from partitions between pens) from the holdings of breeding pigs were analyzed. In five cases, MRSA isolates were detected, with a prevalence of 1.8%. All the isolated strains belonged to a single sequence type ST398. It may be said that at present, the prevalence of MRSA on pig farms does not pose a significant general epidemiological risk for the human population.

scholarly journals Update on the Major Clonal Types of Methicillin-Resistant Staphylococcus aureus in the Czech Republic

2003 ◽  
Vol 41 (11) ◽  
pp. 4998-5005 ◽  
Author(s):  
O. Melter ◽  
M. Aires de Sousa ◽  
P. Urbaskova ◽  
V. Jakubu ◽  
H. Zemlickova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
The Czech Republic ◽  
Methicillin Resistant

scholarly journals Emergence of EMRSA-15 clone in hospitals throughout the Czech Republic

2006 ◽  
Vol 11 (31) ◽  
Author(s):  
O Melter ◽  
P Urbaskova ◽  
V Jakubu ◽  
B Mackova ◽  
H Zemlickova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Epidemic Clone ◽  
The Czech Republic ◽  
Methicillin Resistant

An epidemic clone of methicillin-resistant Staphylococcus aureus (EMRSA-15) ST22-SCCmecIV has been detected in hospitals of all regions of the Czech Republic.

scholarly journals Isolation of Lichens associated Actinomycetes: Determining its antibacterial activity against Multi drug resistant Klebsiella pneumoniae and Methicillin resistant Staphylococcus aureus

2021 ◽  
Vol 23 (06) ◽  
pp. 1489-1509
Author(s):  
VidhyaSri AR ◽  
◽  
Dr. Thamarai Selvi B ◽  
Sanjay Prasad S ◽  
Karkuvelraja R ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Methicillin Resistant ◽  
Rrna Sequencing ◽  
New Antibiotics ◽  
Rapid Emergence

The rapid emergence of drug resistance among pathogenic bacteria, especially multidrug-Resistant bacteria, underlines the need to search for new antibiotics. In the present study, actinomycetes were isolated from lichens collected from coconut trees. The actinomycetes were investigated for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Klebsiella pneumonia in order to identify the potential antibiotic producers.MIC was determined and the organism was identified using 16s rRNA sequencing. Crude action extracts showed 11mm and 9mm against MRSA and Klebsiella pneumonia. The isolated actinomycetes were found to be Streptomyces hygro scopicus subspecies. GC-MS analysis identified several novel compounds. Thus, the isolated actinomycetes can be used for the development of new antibiotics for the treatment of MDR infection.

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