scholarly journals Utility of Amsel Criteria, Nugent Score, and Quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for Diagnosis of Bacterial Vaginosis in Human Immunodeficiency Virus-Infected Women

2005 ◽  
Vol 43 (9) ◽  
pp. 4607-4612 ◽  
Author(s):  
B. E. Sha ◽  
H. Y. Chen ◽  
Q. J. Wang ◽  
M. R. Zariffard ◽  
M. H. Cohen ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Bacterial Vaginosis ◽  
Quantitative Pcr ◽  
Mycoplasma Hominis ◽  
Gardnerella Vaginalis ◽  
Nugent Score ◽  
Immunodeficiency Virus ◽  
Lactobacillus Spp ◽  
Amsel Criteria

scholarly journals Criteria for diagnosis of bacterial vaginosis using the test Femoflor-16

2017 ◽  
Vol 66 (4) ◽  
pp. 57-67 ◽  
Author(s):  
Veronika V. Nazarova ◽  
Elena V. Shipitsyna ◽  
Ekaterina N. Gerasimova ◽  
Alevtina M. Savicheva
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Bacterial Load ◽  
Anaerobic Bacteria ◽  
Microscopic Investigation ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Nugent Score ◽  
Adverse Pregnancy ◽  
Amsel Criteria

Background. Bacterial vaginosis is disturbance of the balance of the vaginal microflora, associated with a number of infectious diseases of the urogenital tract and adverse pregnancy outcomes. In this country, for the detection of vaginal dysbiotic conditions, the test Femoflor-16 (DNA-Technology, Moscow) is widely used, however interpretation algorithms of this test do not include the category of BV. Aim. The study aimed to elaborate diagnostic criteria for the detection of BV using Femoflor-16 test. Materials and methods. Women of reproductive age addressing a gynecologist with vaginal discharge were enrolled in the study. For clinical diagnosis of BV, the Amsel criteria were used, laboratory analysis for BV was performed via microscopic investigation of vaginal discharge using the Nugent score. Samples of vaginal discharge from all women were analyzed with the test Femoflor-16, intended for characterizing vaginal microbiocenosis using multiplex quantitative real-time PCR. Results. A total of 280 women were included in the study. BV was diagnosed in 86 women (31%) using the Amsel criteria, and in 81 women (29%) using the Nugent score. All groups of anaerobic bacteria included in Femoflor-16 test were shown to be associated with BV, with the exception of bacteria of the genus Mobiluncus, which are detected together with phylogenetically related but not BV-associated bacteria of the genus Corynebacterium. A low amount of lactobacilli (< 10% of total bacterial load) coupled with an elevated amount of Gardnerella vaginalis/Prevotella bivia/Porphyromonas (> 1%) and/or Eubacterium (> 2%) and/or Sneathia/Leptotrichia/Fusobacterium (> 0.1%) and/or Megasphaera/Veillonella/Dialister (> 0.1%) and/or Lachnobacterium/Clostridium (> 0.1%) and/or Peptostreptococcus (> 0.1%) and/or Atopobium vaginae (> 0.2%) detected BV with a sensitivity of 99% and specificity of 93%. Conclusions. Criteria for BV diagnosis using the test Femoflor-16 have been elaborated, which enable to detect BV or exclude it with a sensitivity of 99% and specificity of 93%. These criteria for BV and criteria of the test manufacturers for severe anaerobic dysbiosis determine to a large extent the same category of the vaginal microbiocenosis.

scholarly journals Assessment of vaginal microbiota in Brazilian women with and without bacterial vaginosis and comparison with Nugent score

2018 ◽  
Vol 12 (02) ◽  
pp. 127-136 ◽  
Author(s):  
Laura Maria Andrade Oliveira ◽  
Cláudio Galuppo Diniz ◽  
Aline Augusta Sampaio Fernandes ◽  
Daniele Maria Knupp Souza-Sotte ◽  
Michelle Cristine Ribeiro Freitas ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Inverse Correlation ◽  
Vaginal Microbiota ◽  
Gardnerella Vaginalis ◽  
Nugent Score ◽  
Gradient Gel Electrophoresis ◽  
Patient Groups ◽  
Lactobacillus Spp ◽  
Microbiota Structure

Introduction: Bacterial vaginosis (BV) is characterized by the depletion of Lactobacillus spp. population and increase of other species, especially Gardnerella vaginalis and Atopobium vaginae. This study aimed to investigate the vaginal microbiota structure of Brazilian women with and without BV according to Nugent Score and to assess the correlation among Nugent score and the quantification of BV-associated bacteria. Methodology: Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) assay was employed to characterize the vaginal microbiota structure. Quantification of Lactobacillus spp., G. vaginalis, A. vaginae, Mobiluncus sp. and M. hominis were determined by quantitative real-time PCR (qPCR). Results: Clustering by PCR-DGGE revealed differences in microbial structure of the different patient groups. Gardnerella vaginalis, A. vaginae, M. hominis and Mobiluncus sp. were detected at high loads in BV-associated microbiota. Quantification of Lactobacillus spp. showed an inverse correlation with Nugent score while the loads of G. vaginalis, A. vaginae, M. hominis and Mobiluncus sp. indicated a direct correlation with this method. Conclusions: Despite Nugent score is considered the gold standard for BV diagnosis, qPCR stands out as a useful tool for bacteria quantification and an alternative for BV diagnosis. Vaginal microbiota is a complex microbial community although there is a common core among BV and non-BV women. Investigation of vaginal microbiota structure may contribute to the development of tools for diagnosis improvement and therapeutic regimen optimization.

Analisis Pengaruh Pemeriksaan Bacterial Vaginosis (BV) terhadap Kejadian Persalinan Preterm

2021 ◽  
Vol 15 (2) ◽  
pp. 207-217
Author(s):  
Rosita Rosita
Keyword(s):  
Human Immunodeficiency Virus ◽  
Bacterial Vaginosis ◽  
P Value ◽  
Chi Square ◽  
Immunodeficiency Virus

Bacterial vaginosis (BV) merupakan kondisi ketidak seimbangan dari ekosistem vagina, yaitu menurunnya jumlah Lactobacillus. BV biasanya tidak bergejala, namun ketika menimbulkan gejala biasanya disertai dengan keputihan yang berbau. BV yang sudah lama diketahui dapat memberikan komplikasi yang serius, BV juga memberikan dampak pada wanita yang tidak hamil yaitu peningkatan risiko terinfeksi human immunodeficiency virus (HIV) maupun infeksi penyakit kelamin lainnya. Persalinan preterm meningkatkan kematian perinatal sebesar 65-75%. Bayi preterm yang bertahan hidup akan mengalami morbiditas serius jangka pendek maupun jangka panjang. Diperkirakan 50% dari kelahiran preterm spontan terkait dengan infeksi saluran genital. Infeksi pada vagina merupakan faktor risiko persalinan preterm yang paling kuat. Penelitian ini menggunakan desain penelitian kuantitatif deskriptif dengan pendekatan Analisis Data Sekunder. Sample yang digunakan dalam penelitian ini adalah seluruh pasien atau WUS yang terdata atau tercatat dalam laporan di wilayah Kabupaten Bandung sebanyak 83 orang. Dari 291 ibu yang bersalin preterm, yang mengalami positif BV sebanyak 94 orang, sebanyak 109 orang mengeluh keputihan berwarna abnormal, 86 orang dengan konsistensi kental, mengeluh berbau sebanyak 72 orang dan adanya gatal sebanyak 24 orang.  Sedangkan untuk hasil pemeriksaan DUH tubuh, dari 291 ibu preterm sebanyak 76 orang warna pengeluaran cairan vagina berwarna, sebanyak 82 orang konsistensinya lebih kental, sebanyak 101 bau KOH lebih dari 10% dan sebanyak 32 orang Ph vagina lebih dari 4. Hasil analisis bivariat menunjukan ibu yang lahir preterm sebanyak 59,6% hasil pemeriksaan positif BV. Berdasarkan uji Chi-square menunjukan P Value 0,014 < 0,05 maka dapat disimpulkan terdapat pengaruh antara bacterial vaginosis dengan persalinan preterm. sebanyak 16% ibu dengan pengeluaran flor albus berwarna putih menyebabkan kelahiran preterm, konsistensi flor albus yang kental sebanyak 124 ibu dapat mengalami kelahiran preterm. Berdasarkan uji Chi-square menunjukan p Value 0,010 < 0,05 maka dapat disimpulkan terdapat pengaruh aktivitas seksual terhadap bacterial vaginosis. sebanyak 123 orang ibu yang lahir preterm disebabkan oleh bau KOH lebih dari 10%, berdasarkan uji Chi-square menunjukan p Value 0,010 < 0,05 maka dapat disimpulkan terdapat pengaruh DUH tubuh vagina terhadap kehjadian kelahiran preterm. Diharapkan ibu hamil dapat mengetahui tanda gejala BV secara dini dan menghindari faktor-faktor yang dapat memperberat kejadian BV. Diharapkan kepada pada setiap petugas dapat mendeteksi kejadian BV agar menghindari kejadian persalinan / kelahiran preterm.

scholarly journals Bacterial vaginosis: epidemiologic, clinical and diagnostic updates

2018 ◽  
Vol 32 (4) ◽  
Author(s):  
Giorgio Dirani ◽  
Silvia Zannoli ◽  
Maria Federica Pedna ◽  
Francesco Congestrì ◽  
Patrizia Farabegoli ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Current Status ◽  
Gardnerella Vaginalis ◽  
Vaginal Fluid ◽  
Molecular Approach ◽  
Candida Spp ◽  
Kappa Test ◽  
Lactobacillus Spp ◽  
Standard Techniques ◽  
Atopobium Vaginae

Background and aims. Bacterial vaginosis (BV) is one the more frequently identified genital syndrome among childbearing aged women. The basic condition that generates this condition is a modification in the vaginal microbiota. The aim of this paper is to briefly review the current status of the art of BV and to report the results of a pilot study performed with an innovative PCR based technique. Materials and Methods. 36 samples of vaginal fluid routinely submitted for the diagnosis of BV to the Unit of Microbiology – GRHL were comparatively evaluated by standard techniques and with the HP-Vaginiti e Vaginosi NLM kit that simultaneously detects in a quantitative way specific DNA from Candida (albicans, glabrata; krusei, tropicalis), Gardnerella vaginalis, Lactobacillus spp. and Atopobium vaginae. Results and conclusions. Candida spp. has been identified in 8 samples with culture and in 15 with the molecular test. 29 G. vaginalis were found by PCR whereas only in 7 samples a specific prescription for this microbe was present (of which 4 positive). A. vaginae has been identified in 20 samples by the molecular approach and Lactobacillus spp. was identified in 19 samples (by culture) and in 32 by PCR. The overall diagnosis of BV was made in 9 patients by standard techniques and in 7 by applying the molecular approach. (Cohen’s kappa test: 0,84). The findings of this study clearly demonstrate that the joint use of the routine culture- based techniques with the multiplex PCR methods amplifies by far the sensitivity of the overall diagnostic workflow of BV.

scholarly journals Bacterial communities forming the vaginal micro-ecosystem in norm and in bacterial vaginosis

2017 ◽  
Vol 66 (6) ◽  
pp. 30-43
Author(s):  
Veronika V. Nazarova ◽  
Elena V. Shipitsyna ◽  
Kira V. Shalepo ◽  
Alevtina M. Savicheva
Keyword(s):  
Cluster Analysis ◽  
Bacterial Vaginosis ◽  
Bacterial Communities ◽  
Wide Spectrum ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Nugent Score ◽  
Facultative Anaerobes ◽  
Normal Microflora ◽  
Cluster 2

Background. Bacterial vaginosis (BV) is disturbance of the vaginal microbiota, characterized by displacement of lactobacilli with anaerobic bacteria and capable of adversely affecting women’s reproductive health. In the development of BV, a wide spectrum of bacteria substantially differing in their properties is involved. Grouping vaginal bacterial communities into clusters, or types of microbiocenosis, might contribute to understanding of pathogenic mechanisms and elaboration of effective tools for diagnostics and therapy of the disease. Aim. Determination and comparative analysis of clusters of vaginal bacterial communities in norm and in BV. Materials and methods. Women of reproductive age were enrolled in the study. For the diagnosis of BV, the Nugent score was used. Vaginal swab samples from all women were analyzed with the test Femoflor-16, intended for evaluation of the vaginal microbiocenosis using multiplex quantitative real-time PCR. Two-step cluster analysis was applied for grouping bacterial communities. Differences between the clusters were evaluated using pairwise comparisons. Results. Of 280 women enrolled in the study, 172 had normal microflora, 27 – intermediate microflora, 81 – BV. In cluster analysis, 270 samples valid in PCR testing were included. All the vaginal bacterial communities were grouped into 4 clusters. Cluster 1 (n = 171) included cases when the vaginal microflora consisted mostly of lactobacilli. Cluster 2 (n = 11) encompassed cases of domination of aerobic microflora: Enterobacteriaceae, Streptococcus and Staphylococcus. Clusters 3 (n = 57) and 4 (n = 31) were connected with BV and included cases of prevailing of facultative anaerobes (Gardnerella vaginalis, Atopobium vaginae) and obligate anaerobes (Sneathia/Leptotrichia/Fusobacterium, Megasphaera/Veillonella/Dialister, Lachnobacterium/Clostridium), respectively. Nearly all cases of cluster 1 belonged to the category of normal microflora of the Nugent score. The majority of bacterial communities of cluster 2 matched intermediate microflora, cluster 3 – BV category with a score of 7 or 8, cluster 4 – BV category with a score of 9 or 10. The clusters differed significantly in vaginal рН, with the highest values observed for cluster 4. Conclusions. Vaginal bacterial communities are grouped into 4 main clusters, characterized by domination of lactobacilli, aerobes, facultative anaerobes or obligate anaerobes. The clusters belong to different categories of the Nugent score and differ significantly in vaginal pH.

Sign in / Sign up

Export Citation Format

Share Document