Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus

1979 ◽  
Vol 9 (1) ◽  
pp. 120-127
Author(s):  
D W Bradley ◽  
H A Fields ◽  
K A McCaustland ◽  
J E Maynard ◽  
R H Decker ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Protein A ◽  
Competitive Binding ◽  
Immunoglobulin M ◽  
Test Results ◽  
Solid Phase Radioimmunoassay ◽  
Viral Hepatitis A

A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness.

Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay

1977 ◽  
Vol 5 (5) ◽  
pp. 521-530
Author(s):  
D W Bradley ◽  
J E Maynard ◽  
S H Hindman ◽  
C L Hornbeck ◽  
H A Fields ◽  
...  
Keyword(s):  
Viral Hepatitis ◽  
Acute Phase ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Microtiter Plate ◽  
Immunoglobulin M ◽  
Specificity And Sensitivity ◽  
Viral Hepatitis A ◽  
Acute Phase Serum

A modified micro solid-phase radioimmunoassay (RIA) for antibody to hepatitis A virus (anti-HAV) was developed. This double antibody procedure was performed by coating the surface of a polyvinyl microtiter plate "well" with 200 microliter of a 1:1,000 dilution of a patient's test serum. Purified HAV and 125I-labeled immunoglobulin G (IgG) anti-HAV were then sequentially added to form an antibody sandwich. The specificity and sensitivity of the RIA procedure for anti-HAV were verified by examination of coded human and chimpanzee serum specimens. Radioimmunoassay of early-acute-phase serum specimens from human cases of hepatitis A revealed the presence of anti-HAV activity. Differential examination by RIA of IgG and IgM fractions of acute-phase sera from experimentally infected chimpanzees demonstrated that IgM contained the bulk of the anti-HAV activity. A modification of the RIA procedure for anti-HAV (RIA-IgM blocking), incorporating an incubation step with anti-IgM (Mu chain specific), was further shown to differentiate acute- from convalescent-phase hepatitis A sera. This adapted RIA-IgM blocking procedure required less than 1 microliter of a single acute-phase serum specimen for the diagnosis of viral hepatitis A.

scholarly journals Specific immunoglobulin M response to hepatitis A virus determined by solid-phase radioimmunoassay

1980 ◽  
Vol 28 (3) ◽  
pp. 927-936
Author(s):  
S M Lemon ◽  
C D Brown ◽  
D S Brooks ◽  
T E Simms ◽  
W H Bancroft
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Acute Infection ◽  
Type A ◽  
Immunoglobulin M ◽  
Specific Marker ◽  
Acute Type ◽  
Solid Phase Radioimmunoassay ◽  
Control Antigen

Immunoglobulin M antibody to hepatitis A virus (IgM anti-HAV) is found in most patients with acute type A hepatitis. To determine the duration of this IgM response as well as to confirm that IgM anti-HAV is a specific marker for acute infection, we developed a solid-phase radioimmunoassay for IgM anti-HAV. This new assay is 25-fold more sensitive than a conventional blocking radioimmunoassay for anti-HAV, and interference due to rheumatoid factor was eliminated by simultaneously testing sera against virus-free control antigen. Maximum IgM anti-HAV titers (1:6,400 to greater than or equal to 1:51,200) were detected during the first 30 days after the onset of illness. Although the IgM anti-HAV titer subsequently declined 64-fold over the ensuing 90 days, low-titer IgM anti-HAV (1:100 to 1:400) persisted in many sera for 90 to 150 days. Acute sera having an IgM anti-HAV titer of greater than or equal to 1:25,600 possessed a significantly higher mean IgM concentration (492 mg/dl) than acute sera with an IgM anti-HAV titer of less than or equal to 1:12,800 (344 mg/dl; P < 0.05). IgM anti-HAV titers did not correlate with other clinical or laboratory measures of disease severity. Detection of IgM anti-HAV proved to be both a highly specific (>99%) and a sensitive (>99%) method for the diagnosis of type A hepatitis.

Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies.

1993 ◽  
Vol 31 (5) ◽  
pp. 1299-1302 ◽  
Author(s):  
P L Summers ◽  
D R Dubois ◽  
W H Cohen ◽  
P O Macarthy ◽  
L N Binn ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Antibody Capture

scholarly journals Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks

2003 ◽  
Vol 10 (3) ◽  
pp. 492-494 ◽  
Author(s):  
Licel de los Angeles Rodríguez Lay ◽  
Osmany Larralde Díaz ◽  
Raiza Martínez Casanueva ◽  
Aidonis Gutiérrez Moreno
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rapid Diagnosis ◽  
Immunoglobulin M ◽  
Urine Samples ◽  
Test Results ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Urine And Serum

ABSTRACT The main goal of this study was to test the feasibility of using urine for diagnosing hepatitis A virus (HAV) infections. A correlation of 90.78% between the test results of urine and serum samples was obtained. Four outbreaks of hepatitis A were confirmed by testing only urine samples. The levels of anti-HAV immunoglobulin M (IgM) antibodies in urine samples remained stable during 6 months of storage at −70°C but decreased when the samples were stored at 4°C. The results of tests of samples obtained 2 and 6 months after infection suggested that IgM levels decline more rapidly in urine than in serum.

scholarly journals Nationwide seroprevalence of hepatitis A in South Korea from 2009 to 2019

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245162
Author(s):  
Deog-Yong Lee ◽  
Su-Jin Chae ◽  
Seung-Rye Cho ◽  
Wooyoung Choi ◽  
Chang-Ki Kim ◽  
...  
Keyword(s):  
South Korea ◽  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Age Groups ◽  
Immunoglobulin M ◽  
Acute Type ◽  
Prevention Policy ◽  
Patient Report ◽  
Positive Rate

Hepatitis A, an acute type of hepatitis caused by the hepatitis A virus, occurs worldwide. Following the 2009 hepatitis A epidemic in South Korea, patient outbreak reports were collectively converted to an “all-patient report” in 2011, and national immunization programs were introduced for children in 2015. In this study, we aimed to analyze the changes and characteristics of hepatitis A antibody titers in South Korea following the epidemic. The results of hepatitis A antibody tests performed at clinical laboratories from 2009 to 2019 were analyzed based on year, age, region, sex, and medical institution. The average 2009–2018 positive anti-hepatitis A virus immunoglobulin G rate was 51.8%, but it increased (56.06%) in 2019. Significantly different antibody-positive rates were observed based on age: <10 years, 54.5%; 20–29 years, 19.5%; ≥50 years, almost 100%. The positive rate of individuals in their teens and 20s gradually increased, whereas that of those in their 30s and 40s gradually decreased. Males had higher antibody-positive rates than females, and samples from higher-level general hospitals exhibited higher antibody rates. The positive anti-hepatitis A virus immunoglobulin M rates gradually decreased after 2009 and were <1% after 2012. However, a high positive rate of 3.69% was observed in 2019 when there was an epidemic. Anti-hepatitis A virus immunoglobulin G-positive rates were similar throughout the year, but the anti-hepatitis A virus immunoglobulin M-positive rates increased from January, peaked in April, and decreased from July, exhibiting distinct seasonality. This is considered to be related to groundwater pollution during the spring drought season. The introduction of the “all-patient report” and national vaccination program for children has had an effective influence on hepatitis A management. However, for hepatitis A prevention, policy considerations for high-risk age groups with low antibody-positive rates will be necessary.

A Solid-Phase Radioimmunoassay for Detection of IgM Antibodies to Hepatitis A Virus

1979 ◽  
Vol 140 (2) ◽  
pp. 169-175 ◽  
Author(s):  
B. Flehmig ◽  
M. Ranke ◽  
H. Berthold ◽  
H.-J. Gerth
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Igm Antibodies ◽  
Solid Phase Radioimmunoassay
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