scholarly journals A Regulatory Role of the Rnq1 Nonprion Domain for Prion Propagation and Polyglutamine Aggregates

2008 ◽  
Vol 28 (10) ◽  
pp. 3313-3323 ◽  
Author(s):  
Hiroshi Kurahashi ◽  
Masao Ishiwata ◽  
Shoichiro Shibata ◽  
Yoshikazu Nakamura
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Dodecyl Sulfate ◽  
Crucial Role ◽  
De Novo ◽  
Self Regulation ◽  
Sodium Dodecyl ◽  
Protein Conformations ◽  
Chaperone Protein ◽  
Prion Propagation

ABSTRACT Prions are infectious, self-propagating protein conformations. Rnq1 is required for the yeast Saccharomyces cerevisiae prion [PIN +], which is necessary for the de novo induction of a second prion, [PSI +]. Here we isolated a [PSI +]-eliminating mutant, Rnq1Δ100, that deletes the nonprion domain of Rnq1. Rnq1Δ100 inhibits not only [PSI +] prion propagation but also [URE3] prion and huntingtin's polyglutamine aggregate propagation in a [PIN +] background but not in a [pin −] background. Rnq1Δ100, however, does not eliminate [PIN +]. These findings are interpreted as showing a possible involvement of the Rnq1 prion in the maintenance of heterologous prions and polyQ aggregates. Rnq1 and Rnq1Δ100 form a sodium dodecyl sulfate-stable and Sis1 (an Hsp40 chaperone protein)-containing coaggregate in [PIN +] cells. Importantly, Rnq1Δ100 is highly QN-rich and prone to self-aggregate or coaggregate with Rnq1 when coexpressed in [pin −] cells. However, the [pin −] Rnq1-Rnq1Δ100 coaggregate does not represent a prion-like aggregate. These findings suggest that [PIN +] Rnq1-Rnq1Δ100 aggregates interact with other transmissible and nontransmissible amyloids to destabilize them and that the nonprion domain of Rnq1 plays a crucial role in self-regulation of the highly reactive QN-rich prion domain of Rnq1.

Controlling the Pore Size and Connectivity of Alumina-Particle-Stabilized Foams Using Sodium Dodecyl Sulfate: Role of Surfactant Concentration

Langmuir ◽  
2020 ◽  
Vol 36 (35) ◽  
pp. 10331-10340 ◽  
Author(s):  
Yosep Han ◽  
Jiwon Yang ◽  
Minuk Jung ◽  
Seongsoo Han ◽  
Seongmin Kim ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Pore Size ◽  
Surfactant Concentration ◽  
Alumina Particle ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate

Refolding of protein unfolded by gemini surfactants using β-cyclodextrin and sodium dodecyl sulfate in aqueous medium: Study on role of spacer chain of surfactants

2020 ◽  
Vol 300 ◽  
pp. 112238 ◽  
Author(s):  
Sunita Kumari ◽  
Sayantan Halder ◽  
Rishika Aggrawal ◽  
Vinod Kumar Aswal ◽  
Ganapathisubramanian Sundar ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Aqueous Medium ◽  
Gemini Surfactants ◽  
Sodium Dodecyl ◽  
Medium Study ◽  
Dodecyl Sulfate

A comparative study of sodium dodecyl sulfate and freezing/thawing treatment on wheat starch: The role of water absorption

2016 ◽  
Vol 143 ◽  
pp. 149-154 ◽  
Author(s):  
Han Tao ◽  
Pei Wang ◽  
Bao Zhang ◽  
Fengfeng Wu ◽  
Zhengyu Jin ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Comparative Study ◽  
Water Absorption ◽  
Sodium Dodecyl ◽  
Wheat Starch ◽  
Dodecyl Sulfate

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin

1996 ◽  
Vol 8 (7) ◽  
pp. 1097 ◽  
Author(s):  
PD Zalewski ◽  
X Jian ◽  
LL Soon ◽  
WG Breed ◽  
RF Seamark ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Reproductive Tract ◽  
Regional Distribution ◽  
Sodium Dodecyl ◽  
Sperm Head ◽  
Male Reproductive Tract ◽  
Intracellular Location ◽  
And Function ◽  
Triton X

The Zn(II)-specific fluorophore Zinquin was used to determine the regional distribution of free or loosely-bound Zn(II) in mouse spermatozoa. Spermatozoa from the testes exhibited bright fluorescence over the entire head; those from the caput epididymides generally fluoresced more brightly in the post-acrosomal region; and spermatozoa from the caudae epididymides fluoresced less brightly, with foci of fluorescence over the sperm head which were lost after extraction with Triton X-100 and hence appeared to be membrane-associated. Treatment of cauda sperm with sodium dodecyl sulfate resulted in a bright uniform Zinquin fluorescence in the heads, similar to that observed in caput sperm, indicating that the two types of sperm have similar amounts of head Zn(II) but that the availability of Zn(II) for binding Zinquin is different. By contrast, the intensity of tail fluorescence was similar in spermatozoa from different regions of the male reproductive tract and was largely unaffected by Triton X-100 extraction, consistent with an intracellular location. Similar differences were observed between caput sperm and cauda sperm in the rat. It is concluded that visualization and measurement of free or loosely-bound Zn(II) in subcellular compartments of spermatozoa should facilitate investigation of the role of this metal in the development and function of spermatozoa and abnormalities that might accompany infertility and Zn(II) deficiency.

scholarly journals The Effect of Self-Awareness on the Identification of Goal-Related Obstacles

2020 ◽  
Author(s):  
Antonia Kreibich ◽  
Marie Hennecke ◽  
Veronika Brandstaetter-Morawietz
Keyword(s):  
Crucial Role ◽  
Self Regulation ◽  
Experimental Studies ◽  
Personal Goals ◽  
Self Awareness ◽  
Goal Progress ◽  
Individual Approach ◽  
Goal Striving ◽  
Regulatory Aspect

When individuals strive towards personal goals, they may encounter obstacles that could compromise their goal progress and pose a challenge to self-regulation. Coping with obstacles first requires those obstacles to be identified. The purpose of the present studies was to apply an inter-individual approach to this important, but insufficiently studied self-regulatory aspect of goal striving. We therefore examined the role of self-awareness, i.e. paying attention to one’s own feelings, thoughts, and behaviors, for the identification of goal-related obstacles. We measured and manipulated self-awareness in two correlational and two experimental studies (one of them preregistered) and asked participants to identify obstacles to their goals. All studies confirmed the hypothesis that individuals with higher levels of dispositional and situational self-awareness identify more obstacles, both with regard to their idiosyncratic personal goals (Studies 1 and 2) and with regard to a goal in an assigned task during an experiment (Studies 3 and 4). The results indicate that self-awareness plays a crucial role for identifying obstacles. We discuss the implications of our findings for personality and self-regulation research.

Sign in / Sign up

Export Citation Format

Share Document