Ras GTPase-activating protein physically associates with mitogenically active phospholipids.

The physical interaction between GTPase-activating protein (GAP) and lipids has been characterized by two separate analyses. First, bacterially synthesized GAP molecules were found to associate with detergent-mixed micelles containing arachidonic but not with those containing arachidic acid. This association was detected by a faster elution time during molecular exclusion chromatography. Second, GAP molecules within a crude cellular lysate were specifically retained by a column on which certain lipids had been immobilized. The lipids able to retain GAP on such columns were identical to those which were shown previously to be most active in blocking GAP activity. The association between lipids and GAP was dependent upon magnesium ions. Lipids unable to inhibit GAP activity were also unable to physically associate with GAP. The tight association of GAP with these lipids was predicted by and helps to rationalize their ability to inhibit GAP activity.

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Ras GTPase-activating protein physically associates with mitogenically active phospholipids

Molecular and Cellular Biology ◽

10.1128/mcb.11.5.2785-2793.1991 ◽

1991 ◽

Vol 11 (5) ◽

pp. 2785-2793

Author(s):

M H Tsai ◽

M Roudebush ◽

S Dobrowolski ◽

C L Yu ◽

J B Gibbs ◽

...

Keyword(s):

Mixed Micelles ◽

Arachidic Acid ◽

Physical Interaction ◽

Magnesium Ions ◽

Gtpase Activating Protein ◽

Elution Time ◽

Ras Gtpase ◽

Exclusion Chromatography ◽

Tight Association ◽

Molecular Exclusion Chromatography

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The Kidney and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654207 ◽

1970 ◽

Vol 24 (01/02) ◽

pp. 026-032 ◽

Cited By ~ 2

Author(s):

N. A Marsh

Keyword(s):

Molecular Weight ◽

Plasminogen Activator ◽

Enzyme System ◽

Normal Animal ◽

Fibrinolytic Enzyme ◽

The Other ◽

Exclusion Chromatography ◽

Normal Urine ◽

Renal Origin ◽

Molecular Exclusion Chromatography

SummaryMolecular exclusion chromatography was performed on samples of urine from normal and aminonucleoside nephrotic rats. Normal urine contained 2 peaks of urokinase activity, one having a molecular weight of 22,000 and the other around 200,000. Nephrotic urine contained three peaks of activity with MW’s 126,000, 60,000 and 30,000. Plasma activator determined from euglobulin precipitate had a MW. in excess of 200,000. The results indicate that in the normal animal, plasma plasminogen activator does not escape into the urine in substantial quantities but under the conditions of extreme proteinuria there may be some loss through the kidney. The alteration in urokinase output in nephrotic animals indicates a greatly disordered renal fibrinolytic enzyme system.The findings of this study largely support the hypothesis that plasma plasminogen activator of renal origin and urinary plasminogen activator (urokinase) are different molecular species.

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Novel Tumor Suppressive Role of the RAS GTPase-Activating Protein RASA5 to RAS Signaling Perturbation in Human Carcinomas

SSRN Electronic Journal ◽

10.2139/ssrn.3357918 ◽

2019 ◽

Author(s):

Lili Li ◽

Yichao Fan ◽

Xin Huang ◽

Lan Zhong ◽

Xing-sheng Shu ◽

...

Keyword(s):

Ras Signaling ◽

Gtpase Activating Protein ◽

Ras Gtpase ◽

Human Carcinomas

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Tyrosine phosphorylation of Ras GTPase-activating protein stabilizes its association with p62 at membranes of v-Src transformed cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)74450-0 ◽

1993 ◽

Vol 268 (34) ◽

pp. 25728-25734

Author(s):

S Park ◽

R Jove

Keyword(s):

Tyrosine Phosphorylation ◽

Transformed Cells ◽

Gtpase Activating Protein ◽

Ras Gtpase

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Tyrosine phosphorylation of ras GTPase-activating protein does not require association with the epidermal growth factor receptor.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)80641-3 ◽

1993 ◽

Vol 268 (29) ◽

pp. 22010-22019

Author(s):

C Soler ◽

L Beguinot ◽

A Sorkin ◽

G Carpenter

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Tyrosine Phosphorylation ◽

Growth Factor Receptor ◽

Gtpase Activating Protein ◽

Ras Gtpase ◽

Epidermal Growth

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Jatropha Neopauciflora Pax Latex Exhibits Wound-Healing Effect in Normal and Diabetic Mice

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x20986762 ◽

2021 ◽

Vol 26 ◽

pp. 2515690X2098676

Author(s):

Ana Bertha Hernandez-Hernandez ◽

Francisco Javier Alarcon-Aguilar ◽

Mario Garcia-Lorenzana ◽

Marco Aurelio Rodriguez-Monroy ◽

Maria Margarita Canales-Martinez

Keyword(s):

Wound Healing ◽

Healing Process ◽

Wound Healing Process ◽

Diabetic Mice ◽

Wound Area ◽

Oral Infections ◽

Patients With Diabetes ◽

Exclusion Chromatography ◽

Wound Healing Effect ◽

Molecular Exclusion Chromatography

Jatropha neopauciflora is an endemic species of Mexico. Its latex is used to treat wounds, scarring, oral infections, and loose teeth. To date, there are no studies that validate at a morphological level a wound-healing use in diabetes. The present research aimed to evaluate the wound-healing capacity of the latex of J. neopauciflora in the skin of healthy and streptozotocin-induced diabetic mice. Also, a chemical analysis of the latex through molecular exclusion chromatography and HPLC were performed. Male mice ( Mus musculus) of 7-week-old CD1 strain were used. Groups of healthy and diabetic mice were formed. A longitudinal cut of 1 cm was performed on the depilated skin. All treatments were topically applied to the wound area twice a day for ten days. At the end of the experiments, the skin sections were obtained from the wound area and stained with Hematoxylin-Eosin. Then we counted the number of active fibroblasts in all the experimental groups. In normal mice, the latex accelerated the wound-healing process and decreased the number of active fibroblasts, similarly to Recoveron. In diabetic mice, the latex and Recoveron increased the number of active fibroblasts. In normal and diabetic mice, a thin and orderly epidermis was observed. Molecular exclusion chromatography exhibited 58 fractions, 14 of which were subjected to HPLC, to detect catechin, a flavonoid with antioxidant, antimicrobial, and anti-inflammatory properties. J. neopauciflora latex can be useful for wound treatment in patients with diabetes mellitus because it accelerates and promotes the wound-healing process.

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