Yeast virus propagation depends critically on free 60S ribosomal subunit concentration.

Over 30 MAK (maintenance of killer) genes are necessary for propagation of the killer toxin-encoding M1 satellite double-stranded RNA of the L-A virus. Sequence analysis revealed that MAK7 is RPL4A, one of the two genes encoding ribosomal protein L4 of the 60S subunit. We further found that mutants with mutations in 18 MAK genes (including mak1 [top1], mak7 [rpl4A], mak8 [rpl3], mak11, and mak16) had decreased free 60S subunits. Mutants with another three mak mutations had half-mer polysomes, indicative of poor association of 60S and 40S subunits. The rest of the mak mutants, including the mak3 (N-acetyltransferase) mutant, showed a normal profile. The free 60S subunits, L-A copy number, and the amount of L-A coat protein in the mak1, mak7, mak11, and mak16 mutants were raised to the normal level by the respective normal single-copy gene. Our data suggest that most mak mutations affect M1 propagation by their effects on the supply of proteins from the L-A virus and that the translation of the non-poly(A) L-A mRNA depends critically on the amount of free 60S ribosomal subunits, probably because 60S association with the 40S subunit waiting at the initiator AUG is facilitated by the 3' poly(A).

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The TOP2 gene of Trypanosoma brucei: a single-copy gene that shares extensive homology with other TOP2 genes encoding eukaryotic DNA topoisomerase II

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(90)90214-7 ◽

1990 ◽

Vol 38 (1) ◽

pp. 141-150 ◽

Cited By ~ 50

Author(s):

Phyllis R. Strauss ◽

James C. Wang

Keyword(s):

Trypanosoma Brucei ◽

Topoisomerase Ii ◽

Single Copy ◽

Single Copy Gene ◽

Dna Topoisomerase Ii ◽

Dna Topoisomerase ◽

Copy Gene ◽

Genes Encoding ◽

Eukaryotic Dna

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The Dynein Gene Family in Chlamydomonas reinhardtii

Genetics ◽

10.1093/genetics/144.2.569 ◽

1996 ◽

Vol 144 (2) ◽

pp. 569-585 ◽

Cited By ~ 3

Author(s):

Mary E Porter ◽

Julie A Knott ◽

Steven H Myster ◽

Samuel J Farlow

Keyword(s):

Gene Family ◽

Genetic Map ◽

Single Copy ◽

Amino Acid Sequences ◽

Single Copy Gene ◽

Heavy Chains ◽

Length Polymorphisms ◽

Copy Gene ◽

Genes Encoding ◽

Insight Into

Abstract To correlate dynein heavy chain (Dhc) genes with flagellar mutations and gain insight into the function of specific dynein isoforms, we placed eight members of the Dhc gene family on the genetic map of Chlamydomonas. Using a PCR-based strategy, we cloned 11 Dhc genes from Chlamydomonas. Comparisons with other Dhc genes indicate that two clones correspond to genes encoding the alpha and beta heavy chains of the outer dynein arm. Alignment of the predicted amino acid sequences spanning the nucleotide binding site indicates that the remaining nine clones can be subdivided into three groups that are likely to include representatives of the inner-arm Dhc isoforms. Gene-specific probes reveal that each clone represents a single-copy gene that is expressed as a transcript of the appropriate size (>13 kb) sufficient to encode a high molecular weight Dhc polypeptide. The expression of all nine genes is upregulated in response to deflagellation, suggesting a role in axoneme assembly or motility. Restriction fragment length polymorphisms between divergent C. reinhardtii strains have been used to place each Dhr gene on the genetic map of Chlamydomonas. These studies lay the groundwork for correlating defects in different Dhc genes with specific flagellar mutations.

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Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

Physiologia Plantarum ◽

10.1034/j.1399-3054.1992.840410.x ◽

1992 ◽

Vol 84 (4) ◽

pp. 561-567 ◽

Cited By ~ 1

Author(s):

Poul E. Jensen ◽

Michael Kristensen ◽

Tine Hoff ◽

Jan Lehmbeck ◽

Bjarne M. Stummann ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Chlorophyll A ◽

Photosystem I ◽

Single Copy ◽

Type I ◽

Single Copy Gene ◽

Gene Encoding ◽

Copy Gene

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A Mouse Single-Copy Gene,Gtf2i,the Homolog of HumanGTF2I,That Is Duplicated in the Williams–Beuren Syndrome Deletion Region

Genomics ◽

10.1006/geno.1997.5182 ◽

1998 ◽

Vol 48 (2) ◽

pp. 163-170 ◽

Cited By ~ 28

Author(s):

Yu-Ker Wang ◽

Luis A. Pérez-Jurado ◽

Uta Francke

Keyword(s):

Single Copy ◽

Single Copy Gene ◽

Deletion Region ◽

Copy Gene

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Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents

Microorganisms ◽

10.3390/microorganisms9081610 ◽

2021 ◽

Vol 9 (8) ◽

pp. 1610

Author(s):

Christian Klotz ◽

Elke Radam ◽

Sebastian Rausch ◽

Petra Gosten-Heinrich ◽

Toni Aebischer

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Gastrointestinal Disease ◽

Single Copy ◽

Marker Genes ◽

Small Rodent ◽

Analytical Sensitivity ◽

Single Copy Gene ◽

Wild Rodents ◽

Copy Gene

Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.

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Tissue-specific expression of two gamma-glutamyl transpeptidase mRNAs with alternative 5' ends encoded by a single copy gene in the rat.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39983-1 ◽

1990 ◽

Vol 265 (4) ◽

pp. 2352-2357

Author(s):

M N Chobert ◽

O Lahuna ◽

F Lebargy ◽

O Kurauchi ◽

M Darbouy ◽

...

Keyword(s):

Single Copy ◽

Single Copy Gene ◽

Gamma Glutamyl Transpeptidase ◽

Specific Expression ◽

Tissue Specific ◽

Gamma Glutamyl ◽

Tissue Specific Expression ◽

Copy Gene ◽

Glutamyl Transpeptidase

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A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo

Molecular and Cellular Biology ◽

10.1128/mcb.9.1.329-331.1989 ◽

1989 ◽

Vol 9 (1) ◽

pp. 329-331

Author(s):

M Winey ◽

I Edelman ◽

M R Culbertson

Keyword(s):

Saccharomyces Cerevisiae ◽

Genetic Analysis ◽

Single Copy ◽

Single Copy Gene ◽

Copy Gene

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

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Novel chicken actin gene: third cytoplasmic isoform

Molecular and Cellular Biology ◽

10.1128/mcb.5.5.1151-1162.1985 ◽

1985 ◽

Vol 5 (5) ◽

pp. 1151-1162

Author(s):

D J Bergsma ◽

K S Chang ◽

R J Schwartz

Keyword(s):

Nucleotide Sequence ◽

Single Copy ◽

Actin Gene ◽

Single Copy Gene ◽

Gene Diversification ◽

Mrna Transcripts ◽

Copy Gene ◽

Actin Mrna ◽

Distinct Member ◽

Actin Protein

We identified a novel chicken actin gene. The actin protein deduced from its nucleotide sequence very closely resembles the vertebrate cytoplasmic actins; accordingly, we classified this gene as a nonmuscle type. We adopted the convention for indicating the nonmuscle actins of the class Amphibia (Vandekerckhove et al., J. Mol. Biol. 152:413-426) and denoted this gene as type 5. RNA blot analysis demonstrated that the type 5 actin mRNA transcripts accumulate in adult tissues in a pattern indicative of a nonmuscle actin gene. Genomic DNA blots indicated that the type 5 actin is a single copy gene and a distinct member of the chicken actin multigene family. Inspection of the nucleotide sequence revealed many features that distinguished the type 5 gene from all other vertebrate actin genes examined to date. These unique characteristics include: (i) an initiation Met codon preceding an Ala codon, a feature previously known only in plant actins, (ii) a single intron within the 5' untranslated region, with no interruptions in the coding portion of the gene, and (iii) an atypical Goldberg-Hogness box (ATAGAA) preceding the mRNA initiation terminus. These unusual features have interesting implications for actin gene diversification during evolution.

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Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits

Molecular and Cellular Biology ◽

10.1128/mcb.13.5.2835-2845.1993 ◽

1993 ◽

Vol 13 (5) ◽

pp. 2835-2845

Author(s):

M Deshmukh ◽

Y F Tsay ◽

A G Paulovich ◽

J L Woolford

Keyword(s):

Ribosomal Protein ◽

Ribosomal Subunit ◽

Single Copy ◽

5S Rrna ◽

Gene Encoding ◽

Ribosomal Subunits ◽

Ribosomal Protein L1 ◽

The Stability ◽

And Function

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the interaction between L1 and 5S rRNA and to assess the role of L1 in ribosome assembly and function, we cloned the RPL1 gene encoding L1. We have shown that RPL1 is an essential single-copy gene. A conditional null mutant in which the only copy of RPL1 is under control of the repressible GAL1 promoter was constructed. Depletion of L1 causes instability of newly synthesized 5S rRNA in vivo. Cells depleted of L1 no longer assemble 60S ribosomal subunits, indicating that L1 is required for assembly of stable 60S ribosomal subunits but not 40S ribosomal subunits. An L1-5S RNP particle not associated with ribosomal particles was detected by coimmunoprecipitation of L1 and 5S rRNA. This pool of L1-5S RNP remained stable even upon cessation of 60S ribosomal subunit assembly by depletion of another ribosomal protein, L16. Preliminary results suggest that transcription of RPL1 is not autogenously regulated by L1.

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