Remarkable intron and exon sequence conservation in human and mouse homeobox Hox 1.3 genes

A high degree of conservation exists between the Hox 1.3 homeobox genes of mice and humans. The two genes occupy the same relative positions in their respective Hox 1 gene clusters, they show extensive sequence similarities in their coding and noncoding portions, and both are transcribed into multiple transcripts of similar sizes. The predicted human Hox 1.3 protein differs from its murine counterpart in only 7 of 270 amino acids. The sequence similarity in the 250 base pairs upstream of the initiation codon is 98%, the similarity between the two introns, both 960 base pairs long, is 72%, and the similarity in the 3' noncoding region from termination codon to polyadenylation signal is 90%. Both mouse and human Hox 1.3 introns contain a sequence with homology to a mating-type-controlled cis element of the yeast Ty1 transposon. DNA-binding studies with a recombinant mouse Hox 1.3 protein identified two binding sites in the intron, both of which were within the region of shared homology with this Ty1 cis element.

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IN VITRO DNA BINDING STUDIES OF SELECTED HETEROCYCLIC COMPOUNDS

INDIAN DRUGS ◽

10.53879/id.55.12.10994 ◽

2018 ◽

Vol 55 (12) ◽

pp. 24-26

Author(s):

C Akhila ◽

◽

P Lalitha

Keyword(s):

Dna Binding ◽

Heterocyclic Compounds ◽

Uv Spectroscopy ◽

Binding Mode ◽

Spectroscopic Technique ◽

Binding Studies ◽

Base Pairs ◽

Dna Base ◽

Dna Binding Studies

DNA binding studies of selected heterocyclic compounds belonging to the class of quinolinones, substituted quinolinones and thiones were carried out using ct-DNA. The binding nature of the compounds with DNA analyzed using UV-spectroscopy revealed the compounds to be DNA intercalators demonstrating the binding nature of compounds with DNA base pairs. This study is aimed at establishing a facile UV spectroscopic technique to arrive at the binding mode of DNA to ligands.

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A Chimeric IDD4 Repressor Constitutively Induces Immunity in Arabidopsis via the Modulation of Salicylic Acid and Jasmonic Acid Homeostasis

Plant and Cell Physiology ◽

10.1093/pcp/pcz057 ◽

2019 ◽

Vol 60 (7) ◽

pp. 1536-1555 ◽

Cited By ~ 7

Author(s):

Ronny Vï¿½lz ◽

Soon-Kap Kim ◽

Jianing Mi ◽

Kiruthiga G Mariappan ◽

Anna Siodmak ◽

...

Keyword(s):

Salicylic Acid ◽

Jasmonic Acid ◽

Pseudomonas Syringae ◽

Plant Immunity ◽

Stress Hormones ◽

Gene Clusters ◽

Binding Studies ◽

Genome Wide ◽

Wide Range ◽

Dna Binding Studies

Abstract INDETERMINATE DOMAIN (IDD)/BIRD proteins belong to a highly conserved plant-specific group of transcription factors with dedicated functions in plant physiology and development. Here, we took advantage of the chimeric repressor gene-silencing technology (CRES-T, SRDX) to widen our view on the role of IDD4/IMPERIAL EAGLE and IDD family members in plant immunity. The hypomorphic idd4SRDX lines are compromised in growth and show a robust autoimmune phenotype. Hormonal measurements revealed the concomitant accumulation of salicylic acid and jasmonic acid suggesting that IDDs are involved in regulating the metabolism of these biotic stress hormones. The analysis of immunity-pathways showed enhanced activation of immune MAP kinase-signaling pathways, the accumulation of hydrogen peroxide and spontaneous programmed cell death. The transcriptome of nonelicited idd4SRDX lines can be aligned to approximately 40% of differentially expressed genes (DEGs) in flg22-treated wild-type plants. The pattern of DEGs implies IDDs as pivotal repressors of flg22-dependent gene induction. Infection experiments showed the increased resistance of idd4SRDX lines to Pseudomonas syringae and Botrytis cinerea implying a function of IDDs in defense adaptation to hemibiotrophs and necrotrophs. Genome-wide IDD4 DNA-binding studies (DAP-SEQ) combined with DEG analysis of idd4SRDX lines identified IDD4-regulated functional gene clusters that contribute to plant growth and development. In summary, we discovered that the expression of idd4SRDX activates a wide range of defense-related traits opening up the possibility to apply idd4SRDX as a powerful tool to stimulate innate immunity in engineered crops.

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Mutagenesis of the rat insulin II 5'-flanking region defines sequences important for expression in HIT cells.

Molecular and Cellular Biology ◽

10.1128/mcb.9.4.1784 ◽

1989 ◽

Vol 9 (4) ◽

pp. 1784-1789 ◽

Cited By ~ 77

Author(s):

D T Crowe ◽

M J Tsai

Keyword(s):

Gene Expression ◽

Reporter Gene ◽

Sequence Similarity ◽

Flanking Sequence ◽

Base Pairs ◽

Cis Acting ◽

Insulinoma Cell ◽

Chloramphenicol Acetyltransferase Gene ◽

High Degree ◽

Flanking Regions

To define the cis-acting elements important for rat insulin II gene expression, we analyzed the effects of 5' deletions and linker-scanning mutations on the expression of a rat insulin II reporter gene in an insulinoma cell line (HIT). The reporter gene contained 448 base pairs of 5'-flanking sequence joined to the bacterial chloramphenicol acetyltransferase gene. Expression of the 5' deletion mutations indicated that the minimal sequence requirement for efficient expression was 218 base pairs of 5'-flanking sequence, and at least three regions downstream from - 218 were important for transcription. A more precise localization of these elements and the cis-acting sequences in the promoter was achieved by analysis of the expression of 18 linker-scanning mutations. In these studies at least four other regions important for expression of the rat insulin II gene were identified. These findings suggest that the sequences important for rat insulin II and rat insulin I expression may differ significantly despite the high degree of sequence similarity in their 5'-flanking regions.

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Synthesis, Characterization, and DNA Binding Studies of Ruthenium(II) Complexes with 2-Pyridyl-1H-anthra[1,2-d]imidazole-6,11-dione

Australian Journal of Chemistry ◽

10.1071/ch08127 ◽

2008 ◽

Vol 61 (9) ◽

pp. 732 ◽

Cited By ~ 8

Author(s):

Yi-Xian Yuan ◽

Yi-Can Wang ◽

Long Jiang ◽

Feng Gao ◽

Si-Min Liang ◽

...

Keyword(s):

Dna Binding ◽

Imidazole Ring ◽

Spectroscopic Properties ◽

Spectroscopic Studies ◽

X Ray Diffraction ◽

Binding Studies ◽

Base Pairs ◽

Ancillary Ligands ◽

Dna Base ◽

Dna Binding Studies

Three novel asymmetric ruthenium(ii) complexes, [Ru(bpy)2(PAIDH)]2+ 1, [Ru(phen)2(PAIDH)]2+ 2, and [Ru(dmp)2(PAIDH)]2+ 3 (bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, dmp = 2,9-dimethyl-1,10-phenanthroline, PAIDH = 2-pyridyl-1H-anthra[1,2-d]imidazole-6,11-dione), have been synthesized and characterized. The structure of the deprotonated complex [Ru(dmp)2(PAID)]+ 4 has been determined by single-crystal X-ray diffraction techniques, and the anthraquinone moiety is approximately coplanar with the imidazole ring. The DNA binding properities of complexes 1, 2, and 3 to calf-thymus DNA (CT-DNA) were investigated. Spectroscopic studies and viscosity experiments suggest that the RuII complexes intercalate into DNA base pairs by the extended anthraquinone unit, and the ancillary ligands have significant effects on the spectroscopic properties and DNA binding behaviour of the RuII complexes.

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Mutagenesis of the rat insulin II 5'-flanking region defines sequences important for expression in HIT cells

Molecular and Cellular Biology ◽

10.1128/mcb.9.4.1784-1789.1989 ◽

1989 ◽

Vol 9 (4) ◽

pp. 1784-1789

Author(s):

D T Crowe ◽

M J Tsai

Keyword(s):

Gene Expression ◽

Reporter Gene ◽

Sequence Similarity ◽

Flanking Sequence ◽

Base Pairs ◽

Cis Acting ◽

Insulinoma Cell ◽

Chloramphenicol Acetyltransferase Gene ◽

High Degree ◽

Flanking Regions

To define the cis-acting elements important for rat insulin II gene expression, we analyzed the effects of 5' deletions and linker-scanning mutations on the expression of a rat insulin II reporter gene in an insulinoma cell line (HIT). The reporter gene contained 448 base pairs of 5'-flanking sequence joined to the bacterial chloramphenicol acetyltransferase gene. Expression of the 5' deletion mutations indicated that the minimal sequence requirement for efficient expression was 218 base pairs of 5'-flanking sequence, and at least three regions downstream from - 218 were important for transcription. A more precise localization of these elements and the cis-acting sequences in the promoter was achieved by analysis of the expression of 18 linker-scanning mutations. In these studies at least four other regions important for expression of the rat insulin II gene were identified. These findings suggest that the sequences important for rat insulin II and rat insulin I expression may differ significantly despite the high degree of sequence similarity in their 5'-flanking regions.

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