scholarly journals Complete Genome Sequence of Escherichia coli BL21-AI

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Niketa Bhawsinghka ◽  
Katie F. Glenn ◽  
Roel M. Schaaper

Escherichia coli BL21-AI is a commercially available strain possessing a phage T7-based protein-expression system. A combination of tight regulation and high yield makes it widely used for high-level expression of toxic recombinant proteins. Here, we present the complete genome sequence of BL21-AI and provide insights on its genome.

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Muyiwa A. Akindolire ◽  
Bukola R. Aremu ◽  
Collins N. Ateba

Here, we announce the complete genome sequence of bacteriophage PhiG17, which is virulent to Escherichia coli O157:H7 strains and was isolated from cattle feces in the North West province of South Africa. This report presents the major genetic features of the phage PhiG17 based on its whole-genome sequence.


2019 ◽  
Vol 8 (47) ◽  
Author(s):  
Benjamin T. Jones ◽  
Lauren Lessor ◽  
Chandler O’Leary ◽  
Jason Gill ◽  
Mei Liu

Proteus mirabilis is a pathogen that has been linked to nosocomial infections. Studies on phages infecting P. mirabilis may provide therapeutics for infections caused by antibiotic-resistant strains of this pathogen. Here, we announce the complete genome sequence of a P. mirabilis myophage, Mydo, which is distantly related to Escherichia coli phage rv5.


2019 ◽  
Vol 8 (32) ◽  
Author(s):  
Matthew Theodore ◽  
Lauren Lessor ◽  
Chandler O’Leary ◽  
Rohit Kongari ◽  
Jason Gill ◽  
...  

Enterotoxigenic Escherichia coli (ETEC) is an opportunistic pathogen that commonly causes foodborne illness. Study of bacteriophages against this pathogen could be useful to develop alternative treatment approaches. Here, we present the complete genome sequence of LL11, a T7-like podophage that infects ETEC.


2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Denish Piya ◽  
Lauren Lessor ◽  
Mei Liu ◽  
Jason J. Gill

ABSTRACT Here, we describe the complete genome sequence of siphophage LL5. LL5 is a T1-like phage isolated against enterotoxigenic Escherichia coli, which causes traveler’s diarrhea. LL5 is included as a component phage in the commercial prebiotic product PreforPro.


2019 ◽  
Vol 8 (5) ◽  
Author(s):  
Solange Ngazoa-Kakou ◽  
Yuyu Shao ◽  
Geneviève M. Rousseau ◽  
Audrey A. Addablah ◽  
Denise M. Tremblay ◽  
...  

The lytic Escherichia coli siphophage BRET was isolated from a chicken obtained at a local market in Abidjan, Côte d’Ivoire. Its linear genome sequence consists of 59,550 bp (43.4% GC content) and contains 88 predicted genes, including 4 involved in archaeosine biosynthesis.


2019 ◽  
Vol 8 (40) ◽  
Author(s):  
Tyler Higbee ◽  
Shih-Hung Yang ◽  
Russell Moreland ◽  
Mei Liu ◽  
Jolene Ramsey

Here, we announce the genome of the Escherichia coli 4s siphophage Shashou, which presents similarity to members of the Guernseyvirinae subfamily. Shashou is predicted to use a headful packaging mechanism for its 44,155-bp genome and to encode 77 proteins.


2020 ◽  
Vol 9 (50) ◽  
Author(s):  
Gaylen A. Uhlich ◽  
George C. Paoli ◽  
Siddhartha Kanrar

Escherichia coli serotype O157:H7 strain 20R2R is a derivative of clinical isolate PA20. Prophage excision from the coding region of a PA20 transcription factor restored RpoS-dependent biofilm formation in 20R2R, providing a model for O157:H7 stress adaptation when transitioning between clinical and environmental settings. We report here the complete 20R2R genome sequence.


2019 ◽  
Vol 8 (40) ◽  
Author(s):  
James E. Corban ◽  
Jacob Gramer ◽  
Russell Moreland ◽  
Mei Liu ◽  
Jolene Ramsey

Escherichia coli is a Gram-negative bacterium often found in animal intestinal tracts. Here, we present the genome of the Guernseyvirinae-like E. coli 4s siphophage Snoke. The 44.4-kb genome contains 81 protein-coding genes, for which 33 functions were predicted. The capsid morphogenesis gene in Snoke contains a large intein.


2021 ◽  
Vol 10 (23) ◽  
Author(s):  
Keira L. Stuart ◽  
Darrell O. Bayles ◽  
Sarah M. Shore ◽  
Tracy L. Nicholson

Plasmid-mediated polymyxin resistance encoded by mcr-1 has increased public health concerns due to the potential for rapid horizontal transfer. Here, we report the complete genome sequence of colistin-resistant Escherichia coli Antibiotic Resistance Isolate Bank number 0346, harboring a plasmid-borne mcr-1 gene.


2021 ◽  
Vol 10 (13) ◽  
Author(s):  
A. R. Burmeister ◽  
Denish Piya ◽  
Paul E. Turner

ABSTRACT We report the genome sequence of bacteriophage U136B, which is reliant on the lipopolysaccharide and the antibiotic efflux protein TolC for infection of Escherichia coli and is a useful model for studying trade-offs and trade-ups that shape evolution. Phage U136B has a 49,233-bp genome with 87 predicted genes.


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