scholarly journals Complete Genome Assemblies for Three Variants of the Wolbachia Endosymbiont of Drosophila melanogaster

2019 ◽  
Vol 8 (45) ◽  
Author(s):  
Preston J. Basting ◽  
Casey M. Bergman
Keyword(s):  
Drosophila Melanogaster ◽  
Complete Genome ◽  
Shotgun Sequencing ◽  
Data Sets ◽  
Sequencing Data ◽  
Content Type ◽  
Wolbachia Pipientis ◽  
Long Read ◽  
Wolbachia Endosymbiont ◽  
Genome Assemblies

Here, we report genome assemblies for three strains of Wolbachia pipientis, assembled from unenriched, unfiltered long-read shotgun sequencing data of geographically distinct strains of Drosophila melanogaster. Our simple methodology can be applied to long-read data sets of other Wolbachia-infected species with limited Wolbachia-host lateral gene transfers to produce complete assemblies for this important model symbiont.

scholarly journals Complete genome assemblies for three variants of the Wolbachia endosymbiont of Drosophila melanogaster

2019 ◽  
Author(s):  
Preston J. Basting ◽  
Casey M. Bergman
Keyword(s):  
Drosophila Melanogaster ◽  
Complete Genome ◽  
Shotgun Sequencing ◽  
Sequencing Data ◽  
Wolbachia Pipientis ◽  
Long Read ◽  
Wolbachia Endosymbiont ◽  
Genome Assemblies

ABSTRACTHere we report genome assemblies for three strains of Wolbachia pipientis assembled from unenriched, unfiltered long-read shotgun sequencing data of geographically distinct strains of Drosophila melanogaster. Our simple methodology can be applied to long-read datasets of other Wolbachia-infected species to produce complete assemblies for this important model symbiont.

scholarly journals Complete Genome Sequence of Rubrobacter xylanophilus Strain AA3-22, Isolated from Arima Onsen in Japan

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Natsuki Tomariguchi ◽  
Kentaro Miyazaki
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Hot Spring ◽  
Sequencing Data ◽  
Short Read ◽  
Content Type ◽  
Short Read Sequencing ◽  
Oxford Nanopore ◽  
Long Read

Rubrobacter xylanophilus strain AA3-22, belonging to the phylum Actinobacteria, was isolated from nonvolcanic Arima Onsen (hot spring) in Japan. Here, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.

scholarly journals Complete Genome Sequence of Streptococcus mitis Strain Nm-65, Isolated from a Patient with Kawasaki Disease

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Atsushi Tabata ◽  
Hisashi Ohkuni ◽  
Yasuhiko Itoh ◽  
Yoshitaka Fukunaga ◽  
Toshifumi Tomoyasu ◽  
...  
Keyword(s):  
Kawasaki Disease ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Next Generation Sequencing Data ◽  
Tooth Surface ◽  
Data Sets ◽  
Sequencing Data ◽  
Streptococcus Mitis ◽  
Content Type

ABSTRACT Streptococcus mitis Nm-65 is a human commensal streptococcal strain of the mitis group that was isolated from the tooth surface of a patient with Kawasaki disease. The complete genome sequence of Nm-65 was obtained by means of hybrid assembly, using two next-generation sequencing data sets. The final assembly size was 2,085,837 bp, with 2,039 coding sequences.

scholarly journals Complete Genome Sequence of Vibrio rotiferianus Strain AM7

2020 ◽  
Vol 9 (21) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Apirak Wiseschart ◽  
Kusol Pootanakit ◽  
Kei Kitahara
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The Novel ◽  
Sequencing Data ◽  
Short Read ◽  
Content Type ◽  
Short Read Sequencing ◽  
Oxford Nanopore ◽  
Long Read

ABSTRACT We isolated the novel strain Vibrio rotiferianus AM7 from the shell of an abalone. In this article, we report the complete genome sequence of this organism, which was obtained by combining Oxford Nanopore long-read and Illumina short-read sequencing data.

scholarly journals Complete Genome Sequence of Staphylococcus arlettae Strain P2, Isolated from a Laboratory Environment

2019 ◽  
Vol 8 (45) ◽  
Author(s):  
Hiroki Yu ◽  
Makoto Taniguchi ◽  
Kazuma Uesaka ◽  
Apirak Wiseschart ◽  
Kusol Pootanakit ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Hybrid Approach ◽  
Illumina Miseq ◽  
Sequencing Data ◽  
Content Type ◽  
Short Read Sequencing ◽  
Oxford Nanopore ◽  
Long Read

Staphylococcus arlettae is one coagulase-negative species in the bacterial genus Staphylococcus. Here, we describe the closed complete genome sequence of S. arlettae strain P2, which was obtained using a hybrid approach combining Oxford Nanopore long-read and Illumina MiSeq short-read sequencing data.

scholarly journals Complete Genome Sequence of Multidrug-Resistant Strain Nocardia wallacei FMUON74, Isolated from a Sputum Culture

2020 ◽  
Vol 9 (47) ◽  
Author(s):  
Masahiro Toyokawa ◽  
Makoto Taniguchi ◽  
Kazuma Uesaka ◽  
Keiko Nishimura
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Resistant Strain ◽  
Complete Genome ◽  
Hybrid Approach ◽  
Multidrug Resistant ◽  
Content Type ◽  
Short Read Sequencing ◽  
Long Read ◽  
Multidrug Resistant Strain

ABSTRACT Nocardia wallacei is one of the members of the N. transvalensis complex which possess a highly unique susceptibility pattern. Here, we describe the closed complete genome sequence of the multidrug-resistant strain N. wallacei FMUON74, which was obtained using a hybrid approach combining Nanopore long-read sequencing and Illumina and DNBseq short-read sequencing.

scholarly journals Chromosome-Scale Assembly of the Complete Genome Sequence of Porcisia hertigi, Isolate C119, Strain LV43

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
Hatim Almutairi ◽  
Michael D. Urbaniak ◽  
Michelle D. Bates ◽  
Waleed S. Al-Salem ◽  
Rod J. Dillon ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Content Type ◽  
Long Read ◽  
Central Panama

Porcisia hertigi is a parasitic kinetoplastid first isolated from porcupines ( Coendou rothschildi ) in central Panama in 1965. We present the complete genome sequence of P. hertigi , isolate C119, strain LV43, sequenced using combined short- and long-read technologies. This complete genome sequence will contribute to our knowledge of the parasitic genus Porcisia .

scholarly journals Complete Genome Sequence of SMBL-WEM22, a Halotolerant Strain of Kosakonia cowanii Isolated from Hong Kong Seawater

2021 ◽  
Vol 10 (41) ◽  
Author(s):  
W. E. Moore ◽  
G. K. K. Lai ◽  
S. D. J. Griffin ◽  
F. C. C. Leung
Keyword(s):  
Hong Kong ◽  
Dna Sequences ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Gc Content ◽  
Hybrid Assembly ◽  
Gram Negative ◽  
Content Type ◽  
Facultative Anaerobic ◽  
Long Read

Kosakonia cowanii is a Gram-negative, motile, facultative anaerobic enterobacterium that is found in soil, water, and sewage. K. cowanii SMBL-WEM22 is a halotolerant strain that was isolated from seawater in Hong Kong. The complete genome of SMBL-WEM22 (5,037,617 bp, with a GC content of 55.02%) was determined by hybrid assembly of short- and long-read DNA sequences.

scholarly journals Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Håkon Kaspersen ◽  
Thomas H. A. Haverkamp ◽  
Hanna Karin Ilag ◽  
Øivind Øines ◽  
Camilla Sekse ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Complete Genome ◽  
Flow Cell ◽  
Hybrid Assembly ◽  
Genome Sequences ◽  
Content Type ◽  
Short Reads ◽  
Long Reads ◽  
Long Read ◽  
Hybrid Assemblies

ABSTRACT In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.

scholarly journals Complete Genome Resequencing of Thermus thermophilus Strain TMY by Hybrid Assembly of Long- and Short-Read Sequencing Technologies

2021 ◽  
Vol 10 (46) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Natsuko Tokito
Keyword(s):  
Complete Genome ◽  
Thermus Thermophilus ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Hybrid Assembly ◽  
Genome Resequencing ◽  
Short Read ◽  
Content Type ◽  
Sequencing Technologies ◽  
Long Read

Complete genome resequencing was conducted for Thermus thermophilus strain TMY by hybrid assembly of Oxford Nanopore Technologies long-read and MGI short-read data. Errors in the previously reported genome sequence determined by PacBio technology alone were corrected, allowing for high-quality comparative genomic analysis of closely related T. thermophilus genomes.

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