scholarly journals Grass Carp Reovirus VP56 Allies VP4, Recruits, Blocks, and Degrades RIG-I to More Effectively Attenuate IFN Responses and Facilitate Viral Evasion

2021 ◽  
Author(s):  
Hang Su ◽  
Zhiwei Liao ◽  
Chunrong Yang ◽  
Yongan Zhang ◽  
Jianguo Su
Keyword(s):  
Capsid Protein ◽  
Outer Surface ◽  
Grass Carp ◽  
Cell Attachment ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Black Carp ◽  
Reovirus Infection ◽  
Outer Capsid ◽  
Viral Evasion

Grass carp reovirus (GCRV) fibrin VP56 and major outer capsid protein VP4 inlay and locate on the outer surface of GCRV-II and GCRV-III, which causes tremendous loss in grass carp and black carp industries. Fibrin is involved in cell attachment and plays an important role in reovirus infection.

Development and efficacy of a grass carp reovirus (GCRV) outer capsid protein VP7 subunit vaccine

2012 ◽  
Vol 37 (6) ◽  
pp. 659-664 ◽  
Author(s):  
Shi-ying XU ◽  
Jing-hui LI ◽  
Yong ZOU ◽  
Lin LIU ◽  
Cheng-liang GONG ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Grass Carp ◽  
Subunit Vaccine ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Outer Capsid

scholarly journals Grass Carp Reovirus Major Outer Capsid Protein VP4 Interacts with RNA Sensor RIG-I to Suppress Interferon Response

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 560
Author(s):  
Hang Su ◽  
Chengjian Fan ◽  
Zhiwei Liao ◽  
Chunrong Yang ◽  
Jihong Liu Clarke ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Grass Carp ◽  
Cytopathic Effect ◽  
Interferon Response ◽  
Molecular Function ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Outer Capsid ◽  
Production Industry ◽  
Insight Into

Diseases caused by viruses threaten the production industry and food safety of aquaculture which is a great animal protein source. Grass carp reovirus (GCRV) has caused tremendous loss, and the molecular function of viral proteins during infection needs further research, as for most aquatic viruses. In this study, interaction between GCRV major outer capsid protein VP4 and RIG-I, a critical viral RNA sensor, was screened out by GST pull-down, endogenous immunoprecipitation and subsequent LC-MS/MS, and then verified by co-IP and an advanced far-red fluorescence complementation system. VP4 was proved to bind to the CARD and RD domains of RIG-I and promoted K48-linked ubiquitination of RIG-I to degrade RIG-I. VP4 reduced mRNA and promoter activities of key genes of RLR pathway and sequential IFN production. As a consequence, antiviral effectors were suppressed and GCRV replication increased, resulting in intensified cytopathic effect. Furthermore, results of transcriptome sequencing of VP4 stably expressed CIK (C. idella kidney) cells indicated that VP4 activated the MyD88-dependent TLR pathway. Knockdown of VP4 obtained opposite effects. These results collectively revealed that VP4 interacts with RIG-I to restrain interferon response and assist GCRV invasion. This study lays the foundation for anti-dsRNA virus molecular function research in teleost and provides a novel insight into the strategy of immune evasion for aquatic virus.

Expression of outer capsid protein VP5 of grass carp reovirus in E.coli and analysis of its immunogenicity

2009 ◽  
Vol 24 (6) ◽  
pp. 545-551 ◽  
Author(s):  
Lan-lan Zhang ◽  
Jin-yu Shen ◽  
Cheng-feng Lei ◽  
Chao Fan ◽  
Gui-jie Hao ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Grass Carp ◽  
Outer Capsid Protein ◽  
Grass Carp Reovirus ◽  
Outer Capsid

scholarly journals Reovirus Infection or Ectopic Expression of Outer Capsid Protein  1 Induces Apoptosis Independently of the Cellular Proapoptotic Proteins Bax and Bak

2010 ◽  
Vol 85 (1) ◽  
pp. 296-304 ◽  
Author(s):  
M. L. Wisniewski ◽  
B. G. Werner ◽  
L. G. Hom ◽  
L. J. Anguish ◽  
C. M. Coffey ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Ectopic Expression ◽  
Outer Capsid Protein ◽  
Reovirus Infection ◽  
Outer Capsid

scholarly journals Enhancement of Aquareovirus Infectivity by Treatment with Proteases: Mechanism of Action

1998 ◽  
Vol 72 (4) ◽  
pp. 3387-3389 ◽  
Author(s):  
Thomas H. McPhillips ◽  
David Dinan ◽  
Kothandaraman Subramanian ◽  
Siba K. Samal
Keyword(s):  
Capsid Protein ◽  
Striped Bass ◽  
Mechanism Of Action ◽  
Outer Surface ◽  
Outer Capsid Protein ◽  
Polypeptide Composition ◽  
Protease Digestion ◽  
Outer Capsid

ABSTRACT The effects of protease digestion on the polypeptide composition and on the infectivity of striped bass virus, an aquareovirus, were examined. Both trypsin and chymotrypsin enhanced the infectivity of the virus. Enhancement of infectivity was correlated with the digestion of the outer capsid protein, VP7. These studies support the assertion that VP7 is the outermost capsid protein and suggest that VP4 and VP5 are exposed on the outer surface of infectious particles. The possible role of VP7 in the variation in virulence observed among aquareovirus isolates is discussed.

scholarly journals Structure of the Three N-Terminal Immunoglobulin Domains of the Highly Immunogenic Outer Capsid Protein from a T4-Like Bacteriophage

2011 ◽  
Vol 85 (16) ◽  
pp. 8141-8148 ◽  
Author(s):  
A. Fokine ◽  
M. Z. Islam ◽  
Z. Zhang ◽  
V. D. Bowman ◽  
V. B. Rao ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Outer Capsid Protein ◽  
Outer Capsid

Targeting Heat Shock Protein 70 as an antiviral strategy against grass carp reovirus infection

2018 ◽  
Vol 247 ◽  
pp. 1-9 ◽  
Author(s):  
Li-Peng Shan ◽  
Xiao-Hui Chen ◽  
Fei Ling ◽  
Bin Zhu ◽  
Gao-Xue Wang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Grass Carp ◽  
Heat Shock Protein 70 ◽  
Grass Carp Reovirus ◽  
Reovirus Infection
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