Bottom sediments formed in the water bodies under the technogenic impact are important environmental factors affecting water quality and hydrobiota. Usually they consist of natural and technogenic material and differ from natural formations by their morphology, chemical and lithological composition, physicochemical and biochemical properties. In the present paper we use the term “sediment layer of anthropogenic impact” to define the sediment layer containing technogenic material and/or chemical pollutants. The determination of its location in the sediment cores, its thickness and accumulation chronology is an important scientific problem. In the paper the results of layer by layer study of Cs-137, Am-241, Pb-210 specific activities as well as concentrations of petroleum components, lead and mercury in 48 sediment cores of the Sea of Azov and the Don River are examined. The sediment core layers are dated by radiological methods. In all the sediment cores the peak of Cs-137 specific activity related to the Chernobyl accident was detected. In the Sea of Azov, this peak is located in the upper sediment layer up to 10 cm thick, however, in the delta and in the near-delta part of the Don River, where the sedimentation rates are more important, it is found at 20 to 40 cm depth. Also in certain sediment cores the second peak of Cs-137 related to the global nuclear fallout of the 1960s was found. The most of petroleum components, lead and mercury quantities are concentrated in the upper sediment layer formed in the last 50 to 70 years, i.e. in the period of the most important anthropogenic pressure. So, this complex approach based on the data on the vertical distribution of technogenic radionuclides and common pollutants in the sediment cores made it possible to identify and delineate the sediment layer of high anthropogenic impact as well as to assess the duration of this impact.