Composition of titin isoforms of skeletal and cardiac muscles in pathologies

BIOPHYSICS ◽  
2008 ◽  
Vol 53 (6) ◽  
pp. 592-597 ◽  
Author(s):  
I. M. Vikhlyantsev ◽  
Z. A. Podlubnaya
Keyword(s):  
Titin Isoforms ◽  
Cardiac Muscles

Two structural states of the Z-band in cardiac and skeletal muscle

1989 ◽  
Vol 47 ◽  
pp. 1022-1023
Author(s):  
J.P. Schroeter ◽  
M.A. Goldstein ◽  
J.P. Bretaudiere ◽  
L.H. Michael ◽  
R.L. Sass
Keyword(s):  
Skeletal Muscle ◽  
Cross Section ◽  
Real Space ◽  
Contractile State ◽  
Contour Maps ◽  
False Color ◽  
Grey Scale ◽  
Fourier Filtering ◽  
Cardiac Muscles ◽  
Enhancement Algorithm

We have recently established the existence of two structural states of the Z band lattice in cross section in cardiac as well as in skeletal muscle. The two structural states are related to the contractile state of the muscle. In skeletal muscle at rest, the Z band is in the small square (ss) lattice form, but tetanized muscle exhibits the basket weave (bw) form. In contrast, unstimu- lated cardiac muscle exhibits the bw form, but cardiac muscles exposed to EGTA show the ss form.We have used two-dimensional computer enhancement techniques on digitized electron micrographs to compare each lattice form as it appears in both cardiac and skeletal muscle. Both real space averaging and fourier filtering methods were used. Enhanced images were displayed as grey-scale projections, as contour maps, and in false color.There is only a slight difference between the lattices produced by the two different enhancement techniques. Thus the information presented in these images is not likely to be an artifact of the enhancement algorithm.

scholarly journals COMPARATIVE STUDIES OF LIGHT MEROMYOSIN PARACRYSTALS DERIVED FROM RED, WHITE, AND CARDIAC MUSCLE MYOSINS

1971 ◽  
Vol 49 (3) ◽  
pp. 883-898 ◽  
Author(s):  
A. Nakamura ◽  
F. Sreter ◽  
J. Gergely
Keyword(s):  
Molecular Structure ◽  
Cardiac Muscle ◽  
Functional Properties ◽  
Comparative Studies ◽  
White Muscle ◽  
Staining Pattern ◽  
Uranyl Acetate ◽  
Positive Staining ◽  
Cardiac Muscles

Tryptic and chymotryptic light meromyosin paracrystals from red and cardiac muscles of rabbit show a negative and positive staining pattern with uranyl acetate and phosphotungstate that sharply differs from that of white muscle light meromyosin paracrystals. The main periodicity of about 430 A is the same regardless of the source of light meromyosin. The results are discussed in terms of the molecular structure and the functional properties of various myosins.

scholarly journals The C-proteins of rabbit red, white, and cardiac muscles.

1983 ◽  
Vol 258 (13) ◽  
pp. 8395-8401
Author(s):  
K Yamamoto ◽  
C Moos
Keyword(s):  
Cardiac Muscles ◽  
C Proteins

Dynamic localization of αB-crystallin at the microtubule cytoskeleton network in beating heart cells

2020 ◽  
Vol 168 (2) ◽  
pp. 125-137 ◽  
Author(s):  
Eri Ohto-Fujita ◽  
Saaya Hayasaki ◽  
Aya Atomi ◽  
Soichiro Fujiki ◽  
Toshiyuki Watanabe ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Resonance Energy Transfer ◽  
Focal Adhesions ◽  
Resonance Energy ◽  
Heart Cells ◽  
Striated Muscles ◽  
Slow Skeletal Muscle ◽  
Αb Crystallin ◽  
Cardiac Muscles ◽  
Myoblast Cells

Abstract αB-crystallin is highly expressed in the heart and slow skeletal muscle; however, the roles of αB-crystallin in the muscle are obscure. Previously, we showed that αB-crystallin localizes at the sarcomere Z-bands, corresponding to the focal adhesions of cultured cells. In myoblast cells, αB-crystallin completely colocalizes with microtubules and maintains cell shape and adhesion. In this study, we show that in beating cardiomyocytes α-tubulin and αB-crystallin colocalize at the I- and Z-bands of the myocardium, where it may function as a molecular chaperone for tubulin/microtubules. Fluorescence recovery after photobleaching (FRAP) analysis revealed that the striated patterns of GFP-αB-crystallin fluorescence recovered quickly at 37°C. FRAP mobility assay also showed αB-crystallin to be associated with nocodazole-treated free tubulin dimers but not with taxol-treated microtubules. The interaction of αB-crystallin and free tubulin was further confirmed by immunoprecipitation and microtubule sedimentation assay in the presence of 1–100 μM calcium, which destabilizes microtubules. Förster resonance energy transfer analysis showed that αB-crystallin and tubulin were at 1–10 nm apart from each other in the presence of colchicine. These results suggested that αB-crystallin may play an essential role in microtubule dynamics by maintaining free tubulin in striated muscles, such as the soleus or cardiac muscles.

scholarly journals Force development and intracellular Ca2+ in intact cardiac muscles from gravin mutant mice

2017 ◽  
Vol 807 ◽  
pp. 117-126 ◽  
Author(s):  
Zhitao Li ◽  
Sonal Singh ◽  
Santosh V. Suryavanshi ◽  
Wengang Ding ◽  
Xiaoxu Shen ◽  
...  
Keyword(s):  
Mutant Mice ◽  
Force Development ◽  
Cardiac Muscles

Molecular basis of depression of Ca2+ sensitivity of tension by acid pH in cardiac muscles of the mouse and the rat

1996 ◽  
Vol 2 (4) ◽  
pp. 319-326 ◽  
Author(s):  
X.-L. Ding ◽  
A.B. Akella ◽  
E.H. Sonnenblick ◽  
V.G. Rao ◽  
J. Gulati
Keyword(s):  
Molecular Basis ◽  
Acid Ph ◽  
Cardiac Muscles

“ROLE OF SPECTROSCOPY IN MAGNETIC RESONANCE IMAGING: A CLINICAL REVIEW”

2021 ◽  
pp. 20-23
Author(s):  
R. Adityan ◽  
Sajith Selvaganesan
Keyword(s):  
Magnetic Resonance ◽  
High Sensitivity ◽  
Benign Tumors ◽  
Resonance Spectroscopy ◽  
Clinical Implementation ◽  
Cardiac Muscles ◽  
Biochemical Evaluation ◽  
Malignant And Benign Tumors ◽  
Methylene Group

Magnetic Resonance Spectroscopy (MRS) is used in diagnostic imaging for disease metabolism evaluation. The H MRS is highly used because of the abundance, high sensitivity, etc. The various clinical implementation includes whole-brain MRS is used in measuring metabolites of different brain areas simultaneously. The breast MRS is used in malignant and benign tumors differentiation by the total choline compound. The prostate MRS is used to map the metabolites like citrate, choline, and creatinine. For spinal cord MRS, the myoinositol and N acetyl aspartate were 31 23 1 considered markers for various diseases. The MRS uses nuclei like P, Na, and H for metabolic and biochemical evaluation of cardiac muscles. The liver MRS spectrum has mainly methylene group of lipid, methyl groups of choline, and water. The MRS measures choline, creatinine, lactate, and lipid peaks in uterine leiomyoma and myometrium. Hence there are organ-specic metabolites used as a reference to map the metabolic process by using spectroscopy, making it one of the commonly preferred technique.

Sex differences in the involvement of skeletal and cardiac muscles in myopathic Ano5-/- mice.

2022 ◽  
Author(s):  
Steven Foltz ◽  
Fang Wu ◽  
Nasab Ghazal ◽  
Jennifer Kwong ◽  
H. Criss Hartzell ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Sex Differences ◽  
Meta Analysis ◽  
Elevated Serum ◽  
Research Literature ◽  
Exercise Intolerance ◽  
Membrane Repair ◽  
Recessive Mutations ◽  
Skeletal Muscle Dysfunction ◽  
Cardiac Muscles

Limb-girdle muscular dystrophy R12 (LGMD-R12) is caused by recessive mutations in the Anoctamin-5 gene (ANO5, TMEM16E). Although ANO5 myopathy is not X-chromosome linked, we performed a meta-analysis of the research literature and found that three-quarters of LGMD-R12 patients are males. Females are less likely to present with moderate to severe skeletal muscle and/or cardiac pathology. Because these sex differences could be explained in several ways, we compared males and females in a mouse model of LGMD-R12. This model recapitulates the sex differences in human LGMD-R12. Only male Ano5-/- mice had elevated serum creatine kinase after exercise and exhibited defective membrane repair after laser injury. In contrast, by these measures, female Ano5-/- mice were indistinguishable from wild type. Despite these differences, both male and female Ano5-/- mice exhibited exercise intolerance. While exercise intolerance of male mice can be explained by skeletal muscle dysfunction, echocardiography revealed that Ano5-/- female mice had features of cardiomyopathy that may be responsible for their exercise intolerance. These findings heighten concerns that mutations of ANO5 in humans may be linked to cardiac disease.

Sign in / Sign up

Export Citation Format

Share Document