Study of ectoparasitism of ultramicrobacteria of the genus Kaistia, strains NF1 and NF3 by electron and fluorescence microscopy

Microbiology ◽  
2008 ◽  
Vol 77 (1) ◽  
pp. 47-54 ◽  
Author(s):  
N. E. Suzina ◽  
T. Z. Esikova ◽  
V. N. Akimov ◽  
T. N. Abashina ◽  
V. V. Dmitriev ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Electron And Fluorescence Microscopy

Fluorescence Dynamics of Semiconductor Nanorod Clusters Studied by Correlated Atomic Force, Transmission Electron, and Fluorescence Microscopy

2008 ◽  
Vol 112 (50) ◽  
pp. 19945-19956 ◽  
Author(s):  
Claudia Querner ◽  
Siying Wang ◽  
Ken Healy ◽  
Jessamyn A. Fairfield ◽  
Michael D. Fischbein ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Force Transmission ◽  
Atomic Force ◽  
Transmission Electron ◽  
Electron And Fluorescence Microscopy

scholarly journals SARS-CoV-2 spike protein S1 induces fibrin(ogen) resistant to fibrinolysis: Implications for microclot formation in COVID-19

2021 ◽  
Author(s):  
Lize M Grobbelaar ◽  
Chantelle Venter ◽  
Mare Vlok ◽  
Malebogo Ngoepe ◽  
Gert J Laubscher ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Blood Flow ◽  
Severe Acute Respiratory Syndrome ◽  
Fluorescence Microscopy ◽  
Clinical Relevance ◽  
Structural Changes ◽  
Spike Protein ◽  
Plasma Samples ◽  
Electron And Fluorescence Microscopy ◽  
Scanning Electron

Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) -induced infection, the cause of coronavirus disease 2019 (COVID-19), is characterized by unprecedented clinical pathologies. One of the most important pathologies, is hypercoagulation and microclots in the lungs of patients. Here we study the effect of isolated SARS-CoV-2 spike protein S1 subunit as potential inflammagen sui generis. Using scanning electron and fluorescence microscopy as well as mass spectrometry, we investigate the potential of this inflammagen to interact with platelets and fibrin(ogen) directly to cause blood hypercoagulation.  Using platelet poor plasma (PPP), we show that spike protein may interfere with blood flow.  Mass spectrometry also showed that when spike protein S1 is added to healthy PPP, it results in structural changes to β and γ fibrin(ogen), complement 3, and prothrombin. These proteins were substantially resistant to trypsinization, in the presence of spike protein S1. Here we suggest that, in part, the presence of spike protein in circulation may contribute to the hypercoagulation in COVID-19 positive patients and may cause substantial impairment of fibrinolysis. Such lytic impairment may result in the persistent large microclots we have noted here and previously in plasma samples of COVID-19 patients. This observation may have important clinical relevance in the treatment of hypercoagulability in COVID-19 patients.

scholarly journals Dyskinetoplasty in two species of trypanosomatids

1979 ◽  
Vol 35 (1) ◽  
pp. 185-202
Author(s):  
S.L. Hajduk
Keyword(s):  
Fluorescence Microscopy ◽  
Kinetoplast Dna ◽  
Feulgen Staining ◽  
Crithidia Fasciculata ◽  
Caesium Chloride ◽  
Electron And Fluorescence Microscopy ◽  
Staining Techniques ◽  
Trypanosoma Equiperdum ◽  
In Cells

Dyskinetoplastic cells from both Crithidia fasciculata and Trypanosoma equiperdum lack detectable kinetoplast DNA (kDNA) by conventional staining techniques. Two dyskinetoplastic strains of T. equiperdum, either acriflavine-induced or spontaneously occurring, show normal amounts of kDNA (p = 1.692 g/cm3) in analytical caesium chloride, ultracentrifugation. Electron and fluorescence microscopy of the dyskinetoplastic strains of T. equiperdum suggest that the kDNA network is fragmented and dispersed throughout the mitochondrion. The fragmentation and dispersion of the kDNA, rather than a reduction in the amount of kDNA, is the cause of the lack of kinetoplast staining in the dyskinetoplastic strains of T. equiperdum. Acriflavine-treated cultures of C. fasciculata show a decrease in the amount of kDNA (p = 1.703 g/cm3) corresponding to the percentage of dyskinetoplastic cells in the cultures. Electron and fluorescence microscopy of acriflavine-treated cultures of C. fasciculata show the loss of the kDNA network in cells which lack Giemsa and Feulgen staining, confirming the hypothesis that the kDNA is lost in dyskinetoplastic trypanosomatids from insects. Possible modes of acriflavine action are considered and a proposed mechanism for acriflavine action in trypanosomes from mammals is presented.

Legumain Targeting Peptide Conjugated Fluorescent Porous Silicon Nanoparticles for Breast Cancer Imaging

2016 ◽  
Vol 102 ◽  
pp. 45-50 ◽  
Author(s):  
Jayasree S. Kanathasan ◽  
Varghese Swamy ◽  
Uma Devi Palanisamy ◽  
Ammu Kutty G.K. Radhakrishnan
Keyword(s):  
Porous Silicon ◽  
Fluorescence Microscopy ◽  
Surface Functionalization ◽  
Silicon Nanoparticles ◽  
Linear Chain ◽  
Breast Cancer Imaging ◽  
Microscopy Imaging ◽  
Fluorescence Response ◽  
Electron And Fluorescence Microscopy ◽  
Targeting Peptide

Porous silicon (PSi) with a suite of most desirable biomaterial properties has attracted great attention as a multifunctional nanoplatform for bioimaging and drug delivery. Various surface functionalization treatments have been reported for PSi to use as an active tumor cell targeting nanovector. In this study, we investigated surface functionalization treatments using a peptide that is specific to the emerging biomarker legumain. The PSi nanoparticles were coated with dextran and subsequently two types of legumain targeting peptide, Y-shaped and linear chain, were conjugated to produce the functionalized PSi. The functionalized (ligand-conjugated) PSi materials were characterized for morphology, size, functional groups, and fluorescence response using electron and fluorescence microscopy and vibrational spectroscopy techniques. Fluorescence microscopy imaging with two excitation wavelengths (450 nm and 600 nm) suggests comparable fluorescence response of the conjugated PSi to “bare” PSi and the suitability of the PSi functionalized with peptide for bioimaging.

scholarly journals Invasion of Spores of the Arbuscular Mycorrhizal Fungus Gigaspora decipiens by Burkholderia spp

2003 ◽  
Vol 69 (10) ◽  
pp. 6250-6256 ◽  
Author(s):  
Avram Levy ◽  
Barbara J. Chang ◽  
Lynette K. Abbott ◽  
John Kuo ◽  
Gerry Harnett ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Dna Sequences ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Transmission Electron ◽  
Electron And Fluorescence Microscopy ◽  
Scanning Electron

ABSTRACT Burkholderia species are bacterial soil inhabitants that are capable of interacting with a variety of eukaryotes, in some cases occupying intracellular habitats. Pathogenic and nonpathogenic Burkholderia spp., including B. vietnamiensis, B. cepacia, and B. pseudomallei, were grown on germinating spores of the arbuscular mycorrhizal fungus Gigaspora decipiens. Spore lysis assays revealed that all Burkholderia spp. tested were able to colonize the interior of G. decipiens spores. Amplification of specific DNA sequences and transmission electron microscopy confirmed the intracellular presence of B. vietnamiensis. Twelve percent of all spores were invaded by B. vietnamiensis, with an average of 1.5 × 106 CFU recovered from individual infected spores. Of those spores inoculated with B. pseudomallei, 7% were invaded, with an average of 5.5 × 105 CFU recovered from individual infected spores. Scanning electron and fluorescence microscopy provided insights into the morphology of surfaces of spores and hyphae of G. decipiens and the attachment of bacteria. Burkholderia spp. colonized both hyphae and spores, attaching to surfaces in either an end-on or side-on fashion. Adherence of Burkholderia spp. to eukaryotic surfaces also involved the formation of numerous fibrillar structures.

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