Sugar beet (Beta vulgaris L.) morphogenesis in vitro: Effects of phytohormone type and concentration in the culture medium, type of explants, and plant genotype on shoot regeneration frequency

2006 ◽  
Vol 42 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Ya. V. Mishutkina ◽  
A. K. Gaponenko
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Shoot Regeneration ◽  
Culture Medium ◽  
Plant Genotype ◽  
Regeneration Frequency ◽  
Morphogenesis In Vitro ◽  
In Vitro Effects ◽  
Medium Type

scholarly journals Effect of growth regulators and other components of culture medium on morphogenesis of sugar beet (Beta vulgaris L.) in unfertilised ovule in vitro cultures

2012 ◽  
Vol 65 (4) ◽  
pp. 91-100 ◽  
Author(s):  
Magdalena Tomaszewska-Sowa
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Formation ◽  
In Vitro Cultures ◽  
Solid Media ◽  
The Media ◽  
Do So

The aim of the present research was to optimise the process of plant regeneration from unfertilised ovules in sugar beet (<em>Beta vulgaris </em>L.). A two-stage method was applied which involved preculture in liquid MS medium and culture on solid media. The study evaluated the effectiveness of different groups of growth regulators, agar and gelrite on the morphogenesis of explants. To do so, the culture was maintained on 21 MS media containing auxins (NAA, IBA, 2,4-D), cytokinins (BAP, KIN, TDZ), antiauxin (TIBA) as well as solidifying substances at different concentrations and combinations. Varied effectiveness of callus and shoot formation was observed depending on the medium used. The highest efficiency of shoot formation was recorded on the media containing 4.4 μM BAP, 0.09 M sucrose, and 0.04% gelrite. The formation of roots was induced when exposed to 14.8 μM IBA and 0.049 μM 2iP, and the percentage of rooted shoots ranged from 0.0 to 65.2%, depending on the growth regulator combination used for shoot regeneration.

Callus Induction and Shoot Organogenesis in Two Sugar Beet (Beta vulgaris L.) Breeding Lines in vitro Cultured

2013 ◽  
Vol 12 (4) ◽  
pp. 168-178 ◽  
Author(s):  
Farhad Taghipour ◽  
Narges Janalizade ◽  
Maryam Eshrati ◽  
Taraneh Hassanzade ◽  
Fahrul Huyop
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Callus Induction ◽  
Shoot Organogenesis ◽  
Breeding Lines

scholarly journals Methods of creating homozygous lines as breeding genotypes in sugar beet (Beta vulgaris) with apozygotic method of seed reproduction

Bioenergy ◽  
2021 ◽  
Author(s):  
M. V. Roik ◽  
N. S. Kovalchuk ◽  
O. A. Zinchenko ◽  
L. H. Fedoroshchak ◽  
V. I. Vlasiuk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Sugar Beets ◽  
Alloplasmic Lines ◽  
Total Percentage ◽  
A Value ◽  
Open Ground ◽  
Genome Ploidy ◽  
Selection Of

Purpose. Investigation of cytogenetic aspects of embryological processes in the culture of immature apomictic embryos, breeding genotypes of sugar beet with cytoplasmic sterility for differentiation and selection by gametophyte reduced parthenogenesis. Methods. Cytological, biotechnological, fluorescent cytophotometry, field, laboratory. Results. The cytogenetic features of genesis of immature apomictic embryos cells induced in vitro on the 12th, 20th and 22th days of development have been investigated on the basis of CMS apozygotic lines of Beta vulgaris and alloplasmic lines of wild species Beta maritime and Beta patula. Indicators of efficiency of haploid reduced parthenogenesis in vitro in alloplasmic lines significantly exceeded the best technologies in pollen-sterile lines of sugar beet from 3.79% to 6.25% and had a value of 62.2%, 24.8%, and 16.7%, respectively. Stabilization of genome ploidy to diploid was carried out in selected breeding numbers without colchicine, based on evaluation and selection of genome ploidy using software of ploidy analyzer (AP) Partec. Conclusions. The efficiency of haploid reduced parthenogenesis induction in vitro in apozygotic CMS breeding genotypes of sugar beet as affected by genetic potential of cytoplasm and taking into account the total percentage of haploids (50 units; 100 units) and myxoploids (50 units; 100 units) has been investigated. Homozygous lines were created by stabilizing the genome ploidy of haploid and myxoploid micro sprouts during III–IV passages without the use of colchicine. Technologies of rooting in the open ground for use in the breeding process of sugar beets have been improved.

scholarly journals Early studies on the effect of peptide growth factor phytosulfokine-α on Brassica oleracea var. capitata L. protoplasts

2017 ◽  
Vol 86 (3) ◽  
Author(s):  
Agnieszka Kiełkowska ◽  
Adela Adamus
Keyword(s):  
Brassica Oleracea ◽  
Shoot Regeneration ◽  
Liquid Culture ◽  
Culture Medium ◽  
Breeding Lines ◽  
Liquid Culture Medium ◽  
Peptide Growth Factor ◽  
The Media ◽  
Cells Cultured

<span>Phytosulfokines (PSK) are peptidyl growth factors with the potential of inducing cell proliferation. We examined the effect of supplementation of liquid culture medium with 0.1 µM phytosulfokine-α (PSK-α) on protoplast viability and division frequencies in seven accessions of <em>Brassica oleracea</em> var. <em>capitata</em> L., including cultivars and breeding lines. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants and immobilized in calcium-alginate layers. Cabbage protoplast-derived cells cultured in medium supplemented with 0.1 µM of PSK-α had higher viability and division frequencies compared to cells cultured in PSK-α-free control medium. The effect of PSK-α was more pronounced in low-responding accessions (‘Sława z Gołębiewa’, ‘Ramkila F1’, LM, and LM98); however, in two cultivars with very low response (‘Badger Shipper’ and ‘Oregon 123’), although the division frequencies in the media supplemented with PSK-α were increased over the control, the differences were not significant. Obtained callus colonies were subjected to regeneration. PSK-α supplemented into the liquid culture medium had an indirect effect on shoot regeneration by inducing sustained cell divisions leading to an increase in shoot regeneration in Sława z Gołębiewa and both breeding lines.</span>

scholarly journals Plant regeneration from in vitro leaves of four commercial Pyrus species

2008 ◽  
Vol 54 (No. 4) ◽  
pp. 140-148 ◽  
Author(s):  
H. Tang ◽  
Y. Luo ◽  
C. Liu
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Pyrus Communis ◽  
Naphthaleneacetic Acid ◽  
Complete Medium ◽  
Regeneration Frequency ◽  
Regenerated Plants ◽  
Equivalent Conditions

An efficient shoot regeneration from in vitro leaf sections of <I>Pyrus communis</I> Bartlett, <I>P. pyrifolia</I> Shenbuzhi, <I>P. bretschneideri</I> Zaosu and <I>P. ussuriensis</I> Manyuanxiang was successfully developed for use in future transgenic studies. On the basis of regeneration frequency and average shoot numbers, optimal shoot regeneration was obtained on leaf sections of <I>P. communis</I> Bartlett when cultured on Murashige and Skoog complete medium containing 6.0 mg/l BA (6-benzyladenine) and 0.1 mg/l NAA (&alpha;-naphthaleneacetic acid), while Quoirin and Lepoivre complete medium supplemented with 1.0 mg/l TDZ [thidiazuron (N-phenyl-N<sup>1</sup>-1,2,3-thiadiazol-5-ylurea)] and 0.1 mg/l NAA was found best for <I>P. pyrifolia</I> Shenbuzhi, and Nitsch and Nitsch complete medium containing 3.0 mg/l TDZ and 0.1 mg/l NAA or 0.2 mg/l IAA was suitable for<I>P. bretschneideri</I> Zaosu or <I>P. ussuriensis</I> Manyuanxiang, respectively. After cutting the leaves into three sections perpendicular to the midrib and culturing under the equivalent conditions, regeneration occurred more frequently on basal sections than middle sections, and no shoots formed on apical sections. A ratio of NH<sup>+</sup><sub>4</sub>-N/NO<sup>-</sup><sub>3</sub>- N of 1:2~1:7 was found beneficial for shoot regeneration. 75.0–87.5% of proliferating shoots formed roots after 4 weeks of transfer to 1/4 strength of Murashige and Skoog complete medium supplemented with 2.5 mg/l IBA (indole-3-butryric acid) and 30.0 g/l sucrose. Regenerated plants were successfully established under greenhouse conditions.

WHEAT ANDROCLINIC CALLI: DATA OF SCANNING ELECTRONIC MICROSCOPY

2021 ◽  
Vol 0 (4) ◽  
pp. 41-47
Author(s):  
O.A. SELDIMIROVA ◽  
Keyword(s):  
Culture Medium ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Spring Soft Wheat ◽  
Fundamental Possibility ◽  
Morphological Status ◽  
Different Types ◽  
Morphogenesis In Vitro ◽  
Morphogenic Callus

The processes of formation different types of calli, as well as the morphogenesis pathways in morphogenic calli, were studied by scanning electron microscopy (SEM) during anther culture in vitro in hybrid line Fotos of spring soft wheat. The microspore haploid origin of calli has been proven. The morphological status of the obtained calli was determined. It was shown that morphogenic callus consists of small densely packed meristematic cells covered with extracellular substance. This type of calli was obtained using a variant of the Potato II induction culture medium, added by 1.0 mg/l synthetic auxin 2,4-D. Nonmorphogenic callus consists of large, elongated, loosely located cells with a smooth surface. This type of calli was obtained using a variant of the Potato II culture medium, added by 2.0 mg/l 2,4-D. It was found that the introduction of various IAA concentrations into the Blaydes nutrient medium for regeneration in morphogenic calli implements the following pathways of morphogenesis in vitro: embryoidogenesis (without IAA addition), gemmorhizogenesis (0.5 mg/l), and rhizogenesis (1.5 mg/l). Revealed degenerative changes in cells of nonmorphogenic calli. The fundamental possibility of regulating of the morphogenesis pathways of in vitro of morphogenic calli in the direction necessary for research in biotechnological research has been confirmed.

Sign in / Sign up

Export Citation Format

Share Document