scholarly journals Early studies on the effect of peptide growth factor phytosulfokine-α on Brassica oleracea var. capitata L. protoplasts

2017 ◽  
Vol 86 (3) ◽  
Author(s):  
Agnieszka Kiełkowska ◽  
Adela Adamus
Keyword(s):  
Brassica Oleracea ◽  
Shoot Regeneration ◽  
Liquid Culture ◽  
Culture Medium ◽  
Breeding Lines ◽  
Liquid Culture Medium ◽  
Peptide Growth Factor ◽  
The Media ◽  
Cells Cultured

<span>Phytosulfokines (PSK) are peptidyl growth factors with the potential of inducing cell proliferation. We examined the effect of supplementation of liquid culture medium with 0.1 µM phytosulfokine-α (PSK-α) on protoplast viability and division frequencies in seven accessions of <em>Brassica oleracea</em> var. <em>capitata</em> L., including cultivars and breeding lines. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants and immobilized in calcium-alginate layers. Cabbage protoplast-derived cells cultured in medium supplemented with 0.1 µM of PSK-α had higher viability and division frequencies compared to cells cultured in PSK-α-free control medium. The effect of PSK-α was more pronounced in low-responding accessions (‘Sława z Gołębiewa’, ‘Ramkila F1’, LM, and LM98); however, in two cultivars with very low response (‘Badger Shipper’ and ‘Oregon 123’), although the division frequencies in the media supplemented with PSK-α were increased over the control, the differences were not significant. Obtained callus colonies were subjected to regeneration. PSK-α supplemented into the liquid culture medium had an indirect effect on shoot regeneration by inducing sustained cell divisions leading to an increase in shoot regeneration in Sława z Gołębiewa and both breeding lines.</span>

scholarly journals Effects of a commercial biocide, kasugamycin and consistency of the culture medium on the in vitro establishment of bamboo1

2019 ◽  
Vol 49 ◽  
Author(s):  
Daniela Werner Ribeiro dos Santos ◽  
Théo Piucco Rocker ◽  
Thiago Sanches Ornellas ◽  
Miguel Pedro Guerra
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
Liquid Culture Medium ◽  
Bambusa Vulgaris ◽  
Phyllostachys Bambusoides ◽  
In Vitro Establishment ◽  
Dendrocalamus Asper

ABSTRACT The contamination by microorganisms and oxidation of explants in the in vitro establishment of bamboo are recurrent problems for its micropropagation. In the present study, effects of the biocide Plant Preservative Mixture (PPM™), the antibiotic kasugamycin and the consistency of the culture medium were evaluated in the in vitro establishment of Bambusa vulgaris,Phyllostachys bambusoides and Dendrocalamus asper. The presence of PPM™ in the culture medium had a significant effect using 2 mL L-1 or 4 mL L-1 concentrations, as well as in the liquid culture medium, increasing the plants established in the autumn. Kasugamycin promoted variable responses for all the three species, depending on the season. There was interaction among the factors, demonstrating that higher rates of viable plants can be obtained by combining different strategies to reduce the oxidation and contamination. For the in vitro establishment of B. vulgaris,P. bambusoides and D. asper, it is recommended to add 2 mL L-1 or 4 mL L-1 of PPM™ to the liquid culture medium.

The usage of cell selection in vitro in the creation of tomato and eggplants breeding lines with resistance to fusarium (Fuzarium oxysporum)

2014 ◽  
Vol 1 (3) ◽  
pp. 15-21
Author(s):  
T. Ivchenko ◽  
S. Kornienko ◽  
T. Miroshnichenko ◽  
H. Mozgovska
Keyword(s):  
Fusarium Wilt ◽  
Liquid Culture ◽  
Cell Selection ◽  
Standard Equipment ◽  
Breeding Lines ◽  
Selective Media ◽  
Effi Ciency ◽  
Egg Plant ◽  
The Media

Aim. The development of a biotechnological system of assessing and selecting the forms of egg-plant and tomato which are tolerant to fusarium wilt in the culture of the tissues, isolated in vitro. Methods. The studies were car- ried out according to the standard biotechnological methods and using the standard equipment. The high-grade and hybrid seeds of egg-plant and tomato genotypes of F 1 –F 4 breeds with different tolerance to fusarium wilt were used in the studies. The cell selection was carried out in the media with different content of the selective agent (20, 40 and 60 % of the total medium volume). Results. It has been determined that the samples can be reliably differentiated in the selective media with liquid culture fi ltrate on the stages of induction and prolifera- tion of callusogenesis according to their resistance in the fi eld. The effective concentrations of the liquid culture fi ltrate of F. oxysporum in the selective media necessary for the selection of resistant callus clones are 20−30 % for acceptable genotypes and 40–50 % and higher for moderately resistant and resistant samples. According to the selective assessment (2012−2013) of initial breeding lines of egg-plant and tomato and the ones, selected via the cell selection, there were 4 highlighted promising lines of egg-plant and 5 lines of tomato that exceeded the control samples and the initial genotypes in their resistance to fusarium and in fertility. Conclusions. The pos- sibility and effi ciency of the biotechnology of accelerated creation of and express-tests on the breeding lines of egg-plant and tomato resistant to F. oxysporum were substantiated and experimentally proved in order to cut the time necessary for the attainment of resistant initial material.

scholarly journals Solubilization of diabase and phonolite dust by filamentous fungus

Revista CERES ◽  
2014 ◽  
Vol 61 (5) ◽  
pp. 740-745
Author(s):  
Juliana Andréia Vrba Brandão ◽  
Maria Leonor Ribeiro Casimiro Lopes-Assad ◽  
Sandra Regina Ceccato-Antonini
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
Titratable Acidity ◽  
Rock Powder ◽  
Fungus Culture ◽  
Liquid Culture Medium ◽  
Calcium Magnesium ◽  
Iron And Manganese ◽  
Rock Dust

The objective of this study was to evaluate the effect of the fungus Aspergillus niger strain CCT4355 in the release of nutrients contained in two types of rock powder (diabase and phonolite) by means of in vitro solubilization trials. The experimental design was completely randomized in a 5 x 4 factorial design with three replications. It was evaluated five treatments (phonolite dust + culture medium; phonolite dust + fungus + culture medium; diabase powder + culture medium; diabase powder + fungus + culture medium and fungus + culture medium) and four sampling dates (0, 10, 20 and 30 days). Rock dust (0.4% w/v) was added to 125 mL Erlenmeyer flasks containing 50 mL of liquid culture medium adapted to A. niger. The flasks were incubated at 30°C for 30 days, and analysis of pH (in water), titratable acidity, and concentrations of soluble potassium, calcium, magnesium, zinc, iron and manganese were made. The fungus A. niger was able to produce organic acids that solubilized ions. This result indicates its potential to alter minerals contained in rock dust, with the ability to interact in different ways with the nutrients. A significant increase in the amount of K was found in the treatment with phonolite dust in the presence of the fungus. The strain CCT4355 of A. niger can solubilize minerals contained in these rocks dust.

scholarly journals Effect of Morphological and Physiological Development on the Acclimatization of in Vitro Plants of Bambusa vulgaris Schrad ex Wendl in Liquid Culture Medium

OALib ◽  
2015 ◽  
Vol 02 (09) ◽  
pp. 1-7 ◽  
Author(s):  
Yudith Garcia-Ramirez ◽  
Mallelyn González-González ◽  
Marisol Freire-Seijo ◽  
Mariana La O-Cárdenas ◽  
Miladys León-Quintana ◽  
...  
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
In Vitro Plants ◽  
Liquid Culture Medium ◽  
Bambusa Vulgaris

scholarly journals Viability of ‘Candidatus Liberibacter asiaticus’ Prolonged by Addition of Citrus Juice to Culture Medium

2014 ◽  
Vol 104 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Jennifer K. Parker ◽  
Sarah R. Wisotsky ◽  
Evan G. Johnson ◽  
Faraj M. Hijaz ◽  
Nabil Killiny ◽  
...  
Keyword(s):  
Grapefruit Juice ◽  
Culture Medium ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemical Characterization ◽  
Candidatus Liberibacter Asiaticus ◽  
Citrus Greening Disease ◽  
Candidatus Liberibacter ◽  
The Media ◽  
Liberibacter Asiaticus

Huanglongbing, or citrus greening disease, is associated with infection by the phloem-limited bacterium ‘Candidatus Liberibacter asiaticus’. Infection with ‘Ca. L. asiaticus’ is incurable; therefore, knowledge regarding ‘Ca. L. asiaticus’ biology and pathogenesis is essential to develop a treatment. However, ‘Ca. L. asiaticus’ cannot currently be successfully cultured, limiting its study. To gain insight into the conditions conducive for growth of ‘Ca. L. asiaticus’ in vitro, ‘Ca. L. asiaticus’ inoculum obtained from seed of fruit from infected pomelo trees (Citrus maxima ‘Mato Buntan’) was added to different media, and cell viability was monitored for up to 2 months using quantitative polymerase chain reaction in conjunction with ethidium monoazide. Media tested included one-third King's B (K), K with 50% juice from the infected fruit, K with 50% commercially available grapefruit juice, and 100% commercially available grapefruit juice. Results show that juice-containing media dramatically prolong viability compared with K in experiments reproduced during 2 years using different juice sources. Furthermore, biofilm formed at the air–liquid interface of juice cultures contained ‘Ca. L. asiaticus’ cells, though next-generation sequencing indicated that other bacterial genera were predominant. Chemical characterization of the media was conducted to discuss possible factors sustaining ‘Ca. L. asiaticus’ viability in vitro, which will contribute to future development of a culture medium for ‘Ca. L. asiaticus’.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

scholarly journals In vitro shoot regeneration through anther culture of Brassica spp.

1970 ◽  
Vol 35 (2) ◽  
pp. 331-341 ◽  
Author(s):  
MA Sayem ◽  
M Maniruzzaman ◽  
SS Siddique ◽  
M Al-Amin
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Maximum Rate ◽  
Media Composition ◽  
Shoot Initiation ◽  
The Media

The experiment was conducted to investigate the performance of three different genotypes (BARI Sarisha-6, BARI Sarisha-8, and BARI Sarisha-11) in two different media viz., MS and B5 with different concentrations of phytohormone (2, 4-D) for callus induction from uninucleate stage anthers of Brassica and subsequent plant regeneration in MS media with different concentrations of phytohormone (BAP and NAA). Among the genotypes, BARI Sarisha-8 showed the best performance for all the parameters of callus induction. The performance of BARI Sarisha-6 was poor compared to others. Maximum rate of callus induction (%) was observed in MS + 0.5 mg/L 2, 4-D followed by B5 + 0.5 mg/L 2,4-D. The media combination MS + 1.0 mg/L BAP 0.3 mg/L 2,4-D showed the best performance for maintenance of calli. Significant variations were observed among the genotypes and media composition for shoot regeneration. Among the genotypes, BARI Sarisha-8 showed the best performance for shoot regeneration followed by BARJ Sarisha-l1. The genotype BARI Sarisha-8 produced higher percent of shoots/calli and required minimum days for shoot initiation. Higher percent calli without shoot were produced by the genotype BARI Sarisha-6. The media combination MS + 2.0 mg/L BAP + 0.5 mg/L NAA showed the best performance for shoot regeneration and required maximum days for shoot initiation. Keywords: Regeneration; BARI Sarisha-6; BARI Sarisha-8; BARI Sarisha-11; anther culture; phytohormone  DOI: 10.3329/bjar.v35i2.5896Bangladesh J. Agril. Res. 35(2) : 331-341, June 2010

Isolation of previously uncultured rumen bacteria by dilution to extinction using a new liquid culture medium

2011 ◽  
Vol 84 (1) ◽  
pp. 52-60 ◽  
Author(s):  
Nikki Kenters ◽  
Gemma Henderson ◽  
Jeyamalar Jeyanathan ◽  
Sandra Kittelmann ◽  
Peter H. Janssen
Keyword(s):  
Liquid Culture ◽  
Culture Medium ◽  
Rumen Bacteria ◽  
Liquid Culture Medium
Sign in / Sign up

Export Citation Format

Share Document