Effect of Spacers on DNA Probe Properties in Hybridization Analysis

2021 ◽  
Vol 47 (6) ◽  
pp. 1345-1347
Author(s):  
R. A. Miftakhov ◽  
S. A. Lapa ◽  
V. E. Kuznetsova ◽  
A. M. Zolotov ◽  
V. A. Vasiliskov ◽  
...  
Keyword(s):  
Dna Probe ◽  
Hybridization Analysis

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

Preparation of nanocones for immobilizing DNA probe by a low-temperature plasma plume

2006 ◽  
Vol 89 (12) ◽  
pp. 121501 ◽  
Author(s):  
Guangliang Chen ◽  
Wenjun Zhao ◽  
Shihua Chen ◽  
Mingyan Zhou ◽  
Wenran Feng ◽  
...  
Keyword(s):  
Low Temperature ◽  
Plasma Plume ◽  
Dna Probe ◽  
Low Temperature Plasma ◽  
Temperature Plasma

Ratiometric Electrochemical Biosensor for the Sensitive Determination of DNA by a Hairpin DNA Probe

2021 ◽  
pp. 1-11
Author(s):  
Haixia Wang ◽  
Xuejiao Wang ◽  
Mingyuan Zhao ◽  
Chao Shi ◽  
Cuiping Ma
Keyword(s):  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Hairpin Dna ◽  
Sensitive Determination

scholarly journals Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21796-21804
Author(s):  
Seo Won Cho ◽  
Hyun Jeong Lim ◽  
Beelee Chua ◽  
Ahjeong Son
Keyword(s):  
Binding Sites ◽  
Dna Probe ◽  
Target Compound ◽  
Single Stranded Dna ◽  
Fluorescence Response ◽  
Dye Binding

Principle of an ssDNA paired aptasensor where extra dye binding sites are created to enhance its fluorescence response.

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