GRUSP, an Universal Stress Protein, Is Involved in Gibberellin-dependent Induction of Flowering in Arabidopsis thaliana

Abstract The effect of T-DNA insertion in the 3'-UTR region of Arabidopsis thaliana At3g58450 gene encoding the Germination-Related Universal Stress Protein (GRUSP) was studied. It was found that under a long-day condition this mutation delays transition to flowering of grusp-115 transgenic line that due to a reduced content of endogenous bioactive gibberellins GA1 and GA3 in comparison to the wild-type plants (Col-0). Exogenous GA accelerated flowering of both lines but did not change the time of difference in the onset of flowering between Col-0 and grusp-115. In addition to changes in GA metabolism, grusp-115 evidently has disturbances in realization of the signal that induces flowering. This is confirmed by the results of gene expression of the floral integrator FLOWERING LOCUS T (FT) and the floral repressor FLOWERING LOCUS C (FLC), which are key flowering regulators and acting opposite. We hypothesize that the formation of grusp-115 phenotype can also be affected by a low expression level of FT due to up-regulated FLC expression.

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The Gene Encoding the Universal Stress Protein AtUSP is Regulated by Phytohormones and Involved in Seed Germination of Arabidopsis thaliana

Doklady Biochemistry and Biophysics ◽

10.1134/s1607672918020151 ◽

2018 ◽

Vol 479 (1) ◽

pp. 105-107 ◽

Cited By ~ 1

Author(s):

D. S. Gorshkova ◽

I. A. Getman ◽

A. S. Voronkov ◽

S. I. Chizhova ◽

Vl. V. Kuznetsov ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Seed Germination ◽

Stress Protein ◽

Gene Encoding ◽

Universal Stress Protein

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Faculty Opinions recommendation of Targeted DNA methylation represses two enhancers of FLOWERING LOCUS T in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.735237563.793559462 ◽

2019 ◽

Author(s):

Yuehui He

Keyword(s):

Dna Methylation ◽

Arabidopsis Thaliana ◽

Flowering Locus T ◽

Flowering Locus

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Isolation and Functional Characterization of MsFTa, a FLOWERING LOCUS T Homolog from Alfalfa (Medicago sativa)

International Journal of Molecular Sciences ◽

10.3390/ijms20081968 ◽

2019 ◽

Vol 20 (8) ◽

pp. 1968 ◽

Cited By ~ 2

Author(s):

Junmei Kang ◽

Tiejun Zhang ◽

Tao Guo ◽

Wang Ding ◽

Ruicai Long ◽

...

Keyword(s):

Flowering Time ◽

Functional Characterization ◽

Flowering Locus T ◽

Over Expression ◽

Long Day ◽

Gfp Fusion Protein ◽

Intron Structure ◽

Flowering Locus ◽

Flowering Regulation

The production of hay and seeds of alfalfa, an important legume forage for the diary industry worldwide, is highly related to flowering time, which has been widely reported to be integrated by FLOWERING LOCUS T (FT). However, the function of FT(s) in alfalfa is largely unknown. Here, we identified MsFTa, an FT ortholog in alfalfa, and characterized its role in flowering regulation. MsFTa shares the conserved exon/intron structure of FTs, and MsFTa is 98% identical to MtFTa1 in Medicago trucatula. MsFTa was diurnally regulated with a peak before the dark period, and was preferentially expressed in leaves and floral buds. Transient expression of MsFTa-GFP fusion protein demonstrated its localization in the nucleus and cytoplasm. When ectopically expressed, MsFTa rescued the late-flowering phenotype of ft mutants from Arabidopsis and M. trucatula. MsFTa over-expression plants of both Arabidopsis and M. truncatula flowered significantly earlier than the non-transgenic controls under long day conditions, indicating that exogenous MsFTa strongly accelerated flowering. Hence, MsFTa functions positively in flowering promotion, suggesting that MsFTa may encode a florigen that acts as a key regulator in the flowering pathway. This study provides an effective candidate gene for optimizing alfalfa flowering time by genetically manipulating the expression of MsFTa.

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Crystal structure of the protein A t3g01520, a eukaryotic universal stress protein‐like protein from arabidopsis thaliana in complex with AMP

Proteins Structure Function and Bioinformatics ◽

10.1002/prot.24821 ◽

2015 ◽

Vol 83 (7) ◽

pp. 1368-1373 ◽

Cited By ~ 7

Author(s):

Do Jin Kim ◽

Eduard Bitto ◽

Craig A. Bingman ◽

Hyun‐Jung Kim ◽

Byung Woo Han ◽

...

Keyword(s):

Crystal Structure ◽

Arabidopsis Thaliana ◽

Protein A ◽

Stress Protein ◽

Universal Stress Protein

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A T-DNA insertion mutant of AtHMA1 gene encoding a Cu transporting ATPase in Arabidopsis thaliana has a defect in the water–water cycle of photosynthesis

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2008.12.002 ◽

2009 ◽

Vol 94 (3) ◽

pp. 205-213 ◽

Cited By ~ 11

Author(s):

Mieko Higuchi ◽

Hiroshi Ozaki ◽

Minami Matsui ◽

Kintake Sonoike

Keyword(s):

Arabidopsis Thaliana ◽

Water Cycle ◽

Insertion Mutant ◽

Gene Encoding ◽

Dna Insertion

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The early-flowering mutant efs is involved in the autonomous promotion pathway of Arabidopsis thaliana

Development ◽

10.1242/dev.126.21.4763 ◽

1999 ◽

Vol 126 (21) ◽

pp. 4763-4770 ◽

Cited By ~ 3

Author(s):

W.J. Soppe ◽

L. Bentsink ◽

M. Koornneef

Keyword(s):

Arabidopsis Thaliana ◽

Flowering Time ◽

Double Mutant ◽

Plant Size ◽

Early Flowering ◽

Wild Type ◽

Late Flowering ◽

Long Day ◽

Short Days ◽

Transition To Flowering

The transition to flowering is a crucial moment in a plant's life cycle of which the mechanism has only been partly revealed. In a screen for early flowering, after mutagenesis of the late-flowering fwa mutant of Arabidopsis thaliana, the early flowering in short days (efs) mutant was identified. Under long-day light conditions, the recessive monogenic efs mutant flowers at the same time as wild type but, under short-day conditions, the mutant flowers much earlier. In addition to its early-flowering phenotype, efs has several pleiotropic effects such as a reduction in plant size, fertility and apical dominance. Double mutant analysis with several late-flowering mutants from the autonomous promotion (fca and fve) and the photoperiod promotion (co, fwa and gi) pathways of flowering showed that efs reduces the flowering time of all these mutants. However, efs is completely epistatic to fca and fve but additive to co, fwa and gi, indicating that EFS is an inhibitor of flowering specifically involved in the autonomous promotion pathway. A vernalisation treatment does not further reduce the flowering time of the efs mutant, suggesting that vernalisation promotes flowering through EFS. By comparing the length of the juvenile and adult phases of vegetative growth for wild-type, efs and the double mutant plants, it is apparent that efs mainly reduces the length of the adult phase.

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KHZ1 and KHZ2, novel members of the autonomous pathway, repress the splicing efficiency of FLC pre-mRNA in Arabidopsis

Journal of Experimental Botany ◽

10.1093/jxb/erz499 ◽

2019 ◽

Vol 71 (4) ◽

pp. 1375-1386 ◽

Cited By ~ 2

Author(s):

Zongyun Yan ◽

Huiying Shi ◽

Yanan Liu ◽

Meng Jing ◽

Yuzhen Han

Keyword(s):

Rna Binding ◽

Rna Binding Proteins ◽

Underlying Mechanism ◽

Crucial Importance ◽

Flowering Locus T ◽

Autonomous Pathway ◽

Long Day ◽

Splicing Efficiency ◽

Flowering Locus ◽

External Signals

Abstract As one of the most important events during the life cycle of flowering plants, the floral transition is of crucial importance for plant propagation and requires the precise coordination of multiple endogenous and external signals. There have been at least four flowering pathways (i.e. photoperiod, vernalization, gibberellin, and autonomous) identified in Arabidopsis. We previously reported that two Arabidopsis RNA-binding proteins, KHZ1 and KHZ2, redundantly promote flowering. However, the underlying mechanism was unclear. Here, we found that the double mutant khz1 khz2 flowered late under both long-day and short-day conditions, but responded to vernalization and gibberellin treatments. The late-flowering phenotype was almost completely rescued by mutating FLOWERING LOCUS C (FLC) and fully rescued by overexpressing FLOWERING LOCUS T (FT). Additional experiments demonstrated that the KHZs could form homodimers or interact to form heterodimers, localized to nuclear dots, and repressed the splicing efficiency of FLC pre-mRNA. Together, these data indicate that the KHZs could promote flowering via the autonomous pathway by repressing the splicing efficiency of FLC pre-mRNA.

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Floral induction in Arabidopsis thaliana by FLOWERING LOCUS T requires direct repression of BLADE-ON-PETIOLE genes by homeodomain protein PENNYWISE

PLANT PHYSIOLOGY ◽

10.1104/pp.15.00960 ◽

2015 ◽

pp. pp.00960.2015 ◽

Cited By ~ 7

Author(s):

Fernando Andres ◽

Maida Romera-Branchat ◽

Rafael Martínez-Gallegos ◽

Vipul Patel ◽

Korbinian Schneeberger ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Floral Induction ◽

Homeodomain Protein ◽

Flowering Locus T ◽

Flowering Locus

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The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana

BMC Plant Biology ◽

10.1186/s12870-020-2266-0 ◽

2020 ◽

Vol 20 (1) ◽

Cited By ~ 4

Author(s):

Fernando Andrés ◽

Atsuko Kinoshita ◽

Naveen Kalluri ◽

Virginia Fernández ◽

Vítor S. Falavigna ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Floral Transition ◽

Sugar Transporter ◽

Flowering Locus T ◽

Flowering Locus

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Involvement of FLOWERING LOCUS T in microgravity response of Arabidopsis thaliana plants under long- and short-day conditions

10.21203/rs.3.rs-400169/v1 ◽

2021 ◽

Author(s):

Lihua Wang ◽

Junyan Xie ◽

Yuanyuan Wu ◽

Chenghong Mou ◽

Yuwei Jiao ◽

...

Keyword(s):

Gene Expression ◽

Arabidopsis Thaliana ◽

Fluorescent Protein ◽

Higher Plants ◽

Reproductive Stage ◽

Vegetative Stage ◽

Green Fluorescent Protein Gene ◽

Flowering Locus T ◽

Flowering Locus ◽

The Impact

Abstract Microgravity have an impact on growth and development of higher plants in space at both vegetative stage and reproductive stage. A great deal of information has been available on the vegetative stage in space, but relatively little is known about the influence of microgravity on plants at the reproductive stage. In this study, we constructed a transgenic Arabidopsis thaliana plants expressing flowering control gene, FLOWERING LOCUS T (FT), together with green fluorescent protein gene(GFP) under control of a heat shock-inducible promoter (HSP17.4), by which we induced FT expression inflight through remote controlling heating shock treatment. Inflight photography data showed that induction of FT expression in plants in space could counteract the impact of microgravity and promote flowering. Whole-genome microarray analysis of gene expression changes in leaves of wild-type and these transgenic plants grown under different photoperiod conditions in space indicated that the function of the photoperiod-related microgravity response genes are mainly involved in protein synthesis and post-translation protein modulation, notably protein phosphorylation. In addition, changes of circadian component gene expression in response to microgravity under different photoperiod indicated that role of circadian oscillator could act as integrators of microgravity response and photoperiodic signals in Arabidopsis plant grown in space.

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