Capillary electrophoresis applied to analysis and characterization of mono-N-acyl-2,6-diaminopimelic acid derivatives

2011 ◽  
Author(s):  
Miloslava Vítovcová ◽  
Jan Hlaváček ◽  
Jan Pícha ◽  
Václav Vaněk ◽  
Jiří Jiráček ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Diaminopimelic Acid

Biochemical characterization of a cortexless mutant of a variant of Bacillus cereus

1976 ◽  
Vol 22 (7) ◽  
pp. 1007-1012 ◽  
Author(s):  
Susanne M. Pearce
Keyword(s):  
Cell Wall ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Bacillus Cereus ◽  
Biochemical Characterization ◽  
Diaminopimelic Acid ◽  
Peptidoglycan Synthesis ◽  
Biochemical Lesion ◽  
Do So

Previous studies on this cortexless mutant of Bacillus cereus var. alesti indicated that the forespore membrane was the site of the biochemical lesion. This hypothesis is supported by the results presented here: fatty acid composition of sporulating cells of the mutant is altered, while in vegetative cells it is comparable to the parent; soluble precursors of peptidoglycan synthesis are accumulated in the mutant, at the time of cortex formation; homogenates of the mutant prepared at the time of cortex formation are unable to incorporate tritiated diaminopimelic acid into peptidoglycan, while homogenates of cells forming germ cell wall do so to an extent comparable to that of the parent; lipid-linked intermediates are formed by the mutant as in the parent. Apparently the mutant is unable either to transfer disaccharide penta-peptide units from the carrier lipid to the growing peptidoglycan acceptor, or to transport lipid-linked intermediates across the forespore membrane.

Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection

2006 ◽  
Vol 1113 (1-2) ◽  
pp. 251-254 ◽  
Author(s):  
Xiangyi Huang ◽  
Jifang Weng ◽  
Fuming Sang ◽  
Xingtao Song ◽  
Chengxi Cao ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantum Dot ◽  
Fluorescent Detection
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