THU0030 Nutritional Status in Relation to Inflammatory and Oxidative Stress Markers: Association with Disease Activity in Patients with Systemic Lupus Erythematosus

Objective: The objective of this study is to investigate the relationship of oxidative stress to fatigue in systemic lupus erythematosus (SLE). Methods: Patients with a confirmed diagnosis of SLE by ACR criteria and healthy controls completed validated questionnaires to assess depression and fatigue. Fatigue was measured with the Fatigue Severity Scale (FSS) and the Profile of Fatigue (Prof-F). Visual analogue scales (VAS) were also used to assess fatigue and pain. Depression was measured with the Center for Epidemiologic Studies Depression Scale (CES-D). Plasma F2-isoprostane was measured with gas chromatography/mass spectroscopy to assess oxidative stress. Evaluation included medical record review, physical exam and calculation of body mass index (BMI), disease activity (SLEDAI) and damage (SLICC) in the SLE patients. Results: Seventy-one SLE patients with low disease activity (mean SLEDAI = 1.62 standard error (SE) 0.37, range 0–8) were compared to 51 controls. Fatigue-limiting physical activity (defined as FSS ≥ 4) was present in 56% of patients and 12% of controls. F2-isoprostane was higher in SLE patients with fatigue compared to not-fatigued SLE subjects ( p = .0076) who were otherwise similar in ethnicity, disease activity and cardiovascular risk factors. Plasma F2-isoprostane was strongly correlated with FSS and Profile of Somatic Fatigue (Prof-S) ( p < .0001), VAS fatigue ( p = .005), CES-D ( p = .008) and with BMI ( p = .0001.) In a multivariate model, F2-isoprostane was a significant predictor of FSS after adjustment for age, BMI, pain and depression ( p = .0002). Conclusion: Fatigue in SLE patients with low disease activity is associated with increased F2-isoprostane. F2-isoprostane could provide a useful biomarker to explore mitochondrial function and the regulation of oxidative pathways in patients with SLE in whom fatigue is a debilitating symptom.

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Assessment of nutritional status and disease activity level in Systemic Lupus Erythematosus patients at a tertiary care hospital

Revista Colombiana de Reumatología (English Edition) ◽

10.1016/j.rcreue.2019.02.001 ◽

2019 ◽

Vol 26 (2) ◽

pp. 97-104

Author(s):

Mervat Essam Behiry ◽

Marwa Rashad Salem ◽

Alshaimaa R. Alnaggar

Keyword(s):

Systemic Lupus Erythematosus ◽

Nutritional Status ◽

Disease Activity ◽

Lupus Erythematosus ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Activity Level ◽

Care Hospital ◽

Systemic Lupus

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Altered redox regulation by Nrf2-Keap1 system in dendritic cells of systemic lupus erythematosus patients

Lupus ◽

10.1177/0961203320950022 ◽

2020 ◽

Vol 29 (12) ◽

pp. 1544-1555

Author(s):

Preeti Gautam ◽

Gurjasmine Kaur ◽

Ankit Tandon ◽

Aman Sharma ◽

Archana Bhatnagar

Keyword(s):

Oxidative Stress ◽

Systemic Lupus Erythematosus ◽

Dendritic Cells ◽

Disease Activity ◽

Lupus Erythematosus ◽

Redox Regulation ◽

Recent Change ◽

Type I ◽

Systemic Lupus ◽

Type I Ifns

Systemic lupus erythematosus (SLE) is an autoimmune disorder associated with inflammation and multiple organ involvement. Individually, dendritic cells (DCs) and oxidative stress have been well discussed for their critical involvement in the pathogenesis of disease but the precise impact of oxidative stress on DCs in relation to SLE disease activity is yet to be scrutinized. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) pathway is the cellular mechanism to combat increased reactive oxygen species (ROS). The current study was framed in order to understand redox regulation in DCs along with an argument in context to disease activity. Here, 23 SLE patients along with 10 healthy controls were enrolled and disease activity was calculated as the recent change in SLEDAI score. We found the percentage of circulating plasmacytoid DCs (pDCs) was increased with an increase in disease activity. Altered DCs functionality along with disease activity was further supported with the differential concentration of Type I IFNs. The disease activity was positively associated with increased levels of ROS. A relevant reason for increased ROS was further explained with the decreased levels of transcription factor Nrf2. Hence, the present study suggests that SLE specific DCs displayed elevation in ROS and this outcome might be due to impaired free radical clearance by Nrf2. Correlation studies further established an association of disease activity with increased ROS, Type I IFNs levels and decreased activity of oxidative stress regulating enzymes.

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The Investigation of Cytokines and Oxidative Stress in Patients with Systemic Lupus Erythematosus

Journal of Medical Biochemistry ◽

10.2478/v10011-007-0028-2 ◽

2007 ◽

Vol 26 (3) ◽

pp. 231-240

Author(s):

Lilika Zvezdanović ◽

Vidosava Đorđević ◽

Vladan Ćosić ◽

Tatjana Cvetković ◽

Slavica Kundalić ◽

...

Keyword(s):

Oxidative Stress ◽

Systemic Lupus Erythematosus ◽

Lupus Erythematosus ◽

Clinical Disease ◽

Therapeutic Interventions ◽

Systemic Lupus ◽

Sod Activity ◽

Antioxidative Status ◽

Healthy Control ◽

And Oxidative Stress

The Investigation of Cytokines and Oxidative Stress in Patients with Systemic Lupus ErythematosusNumerous factors can influence the onset of SLE and development of some clinical disease manifestations with various organ involvements and occurrence of characteristic symptoms and disease signs. This paper studies the balance between proinflammatory and antiinflammatory cytokines, investigates the presence of oxidative stress measuring certain prooxidative factors and determines the activation of antioxidative protection pathways aiming to establish possible correlations between the studied parameters. ELISA, enzymatic spectrophotometry and colorimetric methods were used to determine the above-mentioned parameters. The results obtained indicate that disturbed pro/antioxidative status is associated with the change of antioxidative factors, with the fall od SOD activity and increase of GPx and CAT activity in the erythrocytes of all studied groups of patients. At the same time, the cytokine production was altered, not only compared to the healthy control samples, but also in various clinical disease manifestations. Altered relationships of pro and antiinflammatory cytokines and the consequential disorders of other studied systems provide us with useful strategic targets for diagnostic monitoring and possible therapeutic interventions in SLE patients.

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Inflammatory biomarkers and oxidative stress measurements in patients with systemic lupus erythematosus with or without metabolic syndrome

Lupus ◽

10.1177/0961203311411348 ◽

2011 ◽

Vol 20 (13) ◽

pp. 1356-1364 ◽

Cited By ~ 28

Author(s):

MAB Lozovoy ◽

ANC Simão ◽

MSN Hohmann ◽

TNC Simão ◽

DS Barbosa ◽

...

Keyword(s):

Oxidative Stress ◽

Systemic Lupus Erythematosus ◽

Metabolic Syndrome ◽

Uric Acid ◽

Waist Circumference ◽

Lupus Erythematosus ◽

Inflammatory Biomarkers ◽

Lipid Hydroperoxides ◽

Systemic Lupus ◽

And Oxidative Stress

The aims of the present study were to report the frequency of metabolic syndrome in systemic lupus erythematosus (SLE); to verify differences in inflammatory biomarkers and oxidative stress in SLE patients with or without metabolic syndrome; and to assess which metabolic syndrome components are associated with oxidative stress and disease activity. The study included 58 SLE patients and 105 controls. SLE patients were divided in two groups, with and without metabolic syndrome. 41.4% patients met the criteria for metabolic syndrome compared with 10.5% controls. Patients with SLE and metabolic syndrome had significantly raised serum uric acid, C-reactive protein (CRP), lipid hydroperoxides, and protein oxidation when compared with patients with SLE without metabolic syndrome. Lipid hydroperoxides were correlated with CRP, whereas protein oxidation was associated with waist circumference and uric acid. There was a positive association between serum C3 and C4 and glucose and between C3 and CRP. SLE disease activity index (SLEDAI) scores were positively correlated with body mass index (BMI) and waist circumference (WC). In conclusion, SLE patients have a high prevalence of metabolic syndrome and this syndrome directly contributes to increase inflammatory status and oxidative stress. Inflammatory processes, being overweight/obese, and uric acid may favor oxidative stress increases in patients with SLE and metabolic syndrome. C3 and C4 may have a positive acute-phase protein behavior in patients with SLE.

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